Addgene

We narrowed to 1,192 results for: 5-Oct

Showing: 661 - 690 of 1192 results

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pT2A-EKAREV-NLS

Plasmid

#173856

Purpose

Encoding EKAREV-NLS.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

EKAREV-NLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-HsSENP5

Plasmid

#71468

Purpose

Encodes human full length SENP5 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

Depositor

Jorge Eduardo Azevedo

Article

Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

Insert

SUMO1/sentrin specific peptidase 5 (SENP5 Human)

Use

Tags

Hexahistidine

Expression

Bacterial

Mutation

Gene optimised for E. coli expression (Genscript)

Promoter

T7

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
MSCV-ERK5-AEF

Plasmid

#31816

Depositor

Astar Winoto

Article

Sohn et al Mol Cell Biol. 2005 Oct;25(19):8553-66.

Insert

Erk5 (Mapk7 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

T219A and Y221F

Promoter

PCMV-LTR

Available since

Sept. 20, 2011

Availability

Academic Institutions and Nonprofits only
pPBpuro-LDR

Plasmid

#173857

Purpose

Encoding a part of the rapamycin-induced dimerization system.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

a Lyn11-targeted FRB (LDR)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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CS2 luc Utrn

Plasmid

#13500

Depositor

Stephen Tapscott

Article

Rosenberg et al J Cell Biol. 2006 Oct 9. 175(1):77-85.

Insert

Utrophin (Utrn Mouse)

Use

Luciferase

Tags

Expression

Mutation

Contains 5 copies of nt 10657-10693 of NM_011682.

Promoter

Available since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
pSEED-LoxP-sfGFP:ALFA

Plasmid

#219660

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest method. It is equipped with LoxP sites to remove the 3xP3-dsRED.

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

sfGFP:ALFA SEED Cassette (LoxP)

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pN1-HsSmg5_267-1017-V5-SBP-MBP_V

Plasmid

#147762

Purpose

Mammalian Expression of HsSmg5_267-1017

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5_267-1017 (SMG5 Human)

Use

Tags

Expression

Mammalian

Mutation

one silent mutation C969T compared to the sequenc…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pN1-HsSmg5-V5-SBP-MBP_V

Plasmid

#147768

Purpose

Mammalian Expression of HsSmg5

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5 (SMG5 Human)

Use

Tags

Expression

Mammalian

Mutation

one silent mutation C969T compared to the sequenc…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsSmg5-del133-155_T

Plasmid

#147553

Purpose

Mammalian Expression of HsSmg5-del133-155

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5-del133-155 (SMG5 Human)

Use

Tags

Expression

Mammalian

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsSmg5-G120E_W

Plasmid

#147819

Purpose

Mammalian Expression of HsSmg5-G120E

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5-G120E (SMG5 Human)

Use

Tags

Expression

Mammalian

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pGJM015

Plasmid

#128549

Purpose

Expresses Chimera SFA8+BN

Depositor

Marcos Alcocer

Article

Alcocer et al J Mol Biol. 2004 Oct 22;343(3):759-69. d

Insert

Chimera SFA8+Ber e 5

Use

Tags

Expression

Yeast

Mutation

Promoter

AOX1

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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KHBD00122

Plasmid

#34276

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG3871 (Six4 Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pECE HA AMPKa1 WT

Plasmid

#69504

Purpose

Expresses AMPK alpha1 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

SV40 early promoter

Available since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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pECE HA AMPKa1 AS

Plasmid

#69831

Purpose

Expresses Expresses AMPK alpha 1 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

SV40 early promoter

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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FUW TetO HA-AMPKa1 AS

Plasmid

#69825

Purpose

Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

minimal CMV promoter with tetracycline operator

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pDONOR-SNCAe3-WT

Plasmid

#85847

Purpose

Donor plasmid for SNCA exon3 wild type sequence. lso contains TagBFP and dTomato

Depositor

Jens Schwamborn

Article

Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

Insert

SNCA exon 3 homology arms (SNCA Human)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pnEA-NvM-HsHelz_1475-1642del1595-1609-GB1His6_AH

Plasmid

#148889

Purpose

Bacterial Expression of HsHelz_1475-1642-del1595-1609

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz_1475-1642-del1595-1609 (HELZ Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvM-HsHelz_1475-1642-GB1His6_AH

Plasmid

#148890

Purpose

Bacterial Expression of HsHelz_1475-1642

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz_1475-1642 (HELZ Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvM-HsHelz_1475-1942-GB1His6_AH

Plasmid

#148891

Purpose

Bacterial Expression of HsHelz_1475-1942

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz_1475-1942 (HELZ Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvM-HsHelz_1643-1792del1746-1764-GB1His6_AH

Plasmid

#148892

Purpose

Bacterial Expression of HsHelz_1643-1792-del1746-1764

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz_1643-1792-del1746-1764 (HELZ Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvM-HsHelz_1643-1792-GB1His6_AH

Plasmid

#148893

Purpose

Bacterial Expression of HsHelz_1643-1792

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz_1643-1792 (HELZ Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvM-HsHelz_1793-1914-GB1His6_AH

Plasmid

#148894

Purpose

Bacterial Expression of HsHelz_1793-1914

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz_1793-1914 (HELZ Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvM-HsHelz_1793-1942del1890-1907-GB1His6_AH

Plasmid

#148895

Purpose

Bacterial Expression of HsHelz_1793-1942-del1890-1907

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz_1793-1942-del1890-1907 (HELZ Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvM-HsHELZ_1793-1942-GB1His6_AH

Plasmid

#148896

Purpose

Bacterial Expression of HsHelz_1793-1942

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz_1793-1942 (HELZ Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9-(BB)-2A-Puro-HsHelz-sgRNA_AH

Plasmid

#148897

Purpose

Mammalian Expression of HsHelz-sgRNA

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz-sgRNA (HELZ Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLIB-2STREP-HsHelz_1051-1942-GSSG-MBP_AG

Plasmid

#148795

Purpose

Mammalian Expression of HsHelz_1051-1942-GSSG

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz_1051-1942-GSSG (HELZ Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvM-HsHelz_1051-1474-GB1His6_AG

Plasmid

#148798

Purpose

Bacterial Expression of HsHelz_1051-1474

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz_1051-1474 (HELZ Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-MS2-HA-HsHelz_1475-1942_AH

Plasmid

#148900

Purpose

Mammalian Expression of HsHelz_1475-1942

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz_1475-1942 (HELZ Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-MS2-HA-HsHelz_1-1474_AH

Plasmid

#148880

Purpose

Mammalian Expression of HsHelz_1-1474

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz_1-1474 (HELZ Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsHelz-E795Q_AG

Plasmid

#148800

Purpose

Mammalian Expression of HsHelz-E795Q

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz-E795Q (HELZ Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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