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We narrowed to 5,704 results for: KRAS

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Showing: 661 - 690 of 5704 results

  2. pPB-Ins-TRE3Gp-KRAB-dCas9-ecDHFR-IRES-GFP-EF1Ap-Puro

    Plasmid
    #183410
    Purpose
    piggyBAC-based doxycycline- and trimethoprim-inducible KRAB-dCas9 CRISPR interference plasmid for enhancer and promoter repression in mammalian cells
    Depositor
    Azim Surani
    Article
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Insert
    KRAB-dCas9-ecDHFR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Expression
    Mammalian
    Promoter
    TRE3G
    Available Since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183410-plasmid-map-sequence-id-361256

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  8. pHR-UCOE-SFFV-SID4x-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP

    Plasmid
    #188901
    Purpose
    dCas9 with an N-term Sid4x fusion, a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1) fusion, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element
    Depositor
    Marco Jost, Jonathan Weissman
    Article
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    SID-dCas9-XTEN80-KRAB(Kox1)
    Use
    Lentiviral
    Tags
    HA-2xNLS-XTEN80(linker)-KRAB(Kox1) fusion, P2A-GF…
    Promoter
    SFFV
    Available Since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    188901-plasmid-map-sequence-id-374829

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  11. pBK2047-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA2)

    Plasmid
    #223167
    Purpose
    2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and gRNA2 targeting human/mouse APOE
    Depositor
    Boris Kantor
    Article
    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.
    Insert
    KRAB-MECP2 (MECP2 Human)
    Use
    AAV and CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Encodes aa 204-310 (MECP2)
    Promoter
    EF1a
    Available Since
    Aug. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    223167-plasmid-map-sequence-id-442870

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  16. pBK2046-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA1)

    Plasmid
    #223166
    Purpose
    2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and gRNA1 targeting human/mouse APOE
    Depositor
    Boris Kantor
    Article
    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.
    Insert
    KRAB-MECP2 (MECP2 Human)
    Use
    AAV and CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Encodes aa 204-310 (MECP2)
    Promoter
    EF1a
    Available Since
    Aug. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    223166-plasmid-map-sequence-id-442868

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  23. pJC2528 - CLYBL-Neo-CAGGS-LSL-dCas9-TagBFP-KRAB-CLYBL (LSL-CRISPRi)

    Plasmid
    #250253
    Purpose
    Homology donor for knock-in of CRISPRi system into CLYBL locus with addgene plasmids 62196 and 62197. Lox-Stop-Lox requires cre delivery for activation.
    Depositor
    Jacob Corn
    Article
    Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:
    Insert
    dCas9-mTagBFP-KRAB
    Use
    CRISPR
    Tags
    HA-2xSV40 NLS-mTagBFP-KRAB
    Expression
    Mammalian
    Promoter
    CAGGS
    Available Since
    Jan. 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    250253-plasmid-map-sequence-id-498726

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  24. pCC_10 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139095
    Purpose
    Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    KRAB-dSpCas9-NG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139095-plasmid-map-sequence-id-273892

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  25. pCC_09 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9-NLS-2A-Puro-WPRE

    Plasmid
    #139094
    Purpose
    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    KRAB-dSpCas9
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139094-plasmid-map-sequence-id-273891

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  27. pCC_12 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139097
    Purpose
    Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    KRAB-dxCas9-NG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139097-plasmid-map-sequence-id-273896

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  29. pCC_11 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9-NLS-2A-Puro-WPRE

    Plasmid
    #139096
    Purpose
    Expresses human codon-optimized inactive xCas9 3.7 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    KRAB-dxCas9 3.7
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, A262T, R324L,S409I, E480K, E543D, M6…
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139096-plasmid-map-sequence-id-273894

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Showing: 661 - 690 of 5704 results