We narrowed to 24,051 results for: Nes

1. FUW-hSNCA(D2E)-NE

   Plasmid

   #215488

   Purpose

   Lentiviral expression of mutated alpha-synuclein (2nd amino acid aspartate mutated to glutamate) in mammalian cells

   Depositor

   Ophir Shalem

   Article

   Santhosh Kumar et al Sci Adv. 2024 Feb 9;10(6):eadj4767. doi:

   Insert

   alpha-synuclein (SNCA Human)
   

   Use

   Lentiviral

   Tags

   NE tag

   Expression

   Mammalian

   Mutation

   D (second aa) mutated to E

   Promoter

   Ubc

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGFP-nE-mHP1alpha-delCTE

   Plasmid

   #181896

   Purpose

   Fluorescently tagged HP1alpha, serines in N-terminal extension (NTE) replaced by glutamates, C-terminal deletion

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   nE-mHP1alpha-delCTE (Cbx5 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   S11E, S12E, S13E, S14E, 14 aa C-terminal deletion

   Promoter

   CMV

   Available since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPHR-mCherry-nE-mHP1alpha

   Plasmid

   #181890

   Purpose

   Soluble light-dependent effector protein (HP1alpha, phosphomimetic NTE mutant) for optodroplet formation or recruitment to CIBN localizers

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   PHR-mCherry-nE-mHP1alpha (Cbx5 Mustard Weed, Mouse)
   

   Use

   Tags

   PHR-mCherry

   Expression

   Mammalian

   Mutation

   S11E, S12E, S13E, S14E

   Promoter

   CMV

   Available since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPyCAG-NE-mKate2-IRES-Hph

   Plasmid

   #183616

   Purpose

   Mammalian expression vector: CAG promoter driving expression of nuclear envelope-tethered mKate2 (NLS-mKate2-EmerinTMD). Confers hygromycin B resistance.

   Depositor

   Sally Lowell

   Article

   Blin et al PLoS Biol. 2019 Aug 9;17(8):e3000388. do

   Insert

   mKate2
   

   Use

   Tags

   Human EMD transmembrane domain and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPyCAG-NE-EGFP-IRES-Pac

   Plasmid

   #183617

   Purpose

   Mammalian expression vector: CAG promoter driving expression of nuclear envelope-tethered EGFP (NLS-EGFP-EmerinTMD). Confers puromycin resistance.

   Depositor

   Sally Lowell

   Article

   Malaguti et al Dev Cell. 2019 Jun 11. pii: S1534-5807(1

   Insert

   EGFP
   

   Use

   Tags

   Human EMD transmembrane domain and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPHR-mCherry-nE-mHP1alpha-delCTE

   Plasmid

   #181891

   Purpose

   Soluble light-dependent effector protein (HP1alpha, phosphomimetic NTE mutant with deletion of C-terminal extension)for optodroplet formation or recruitment to CIBN localizers

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   PHR-mCherry-nE-mHP1alpha-delCTE (Cbx5 Mustard Weed, Mouse)
   

   Use

   Tags

   PHR-mCherry

   Expression

   Mammalian

   Mutation

   S11E, S12E, S13E, S14E, 14 aa C-terminal deletion

   Promoter

   CMV

   Available since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Bcr DH domain NE/AA pGEX

   Plasmid

   #38180

   Depositor

   Nora Heisterkamp

   Article

   Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

   Insert

   BCR DH domain N689A/E690A (BCR Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   BCR DH domain N689A/E690A

   Promoter

   Available since

   Oct. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

8. Abr DH domain NE/AA pGEX

   Plasmid

   #38182

   Depositor

   Nora Heisterkamp

   Article

   Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

   Insert

   ABR DH domain N282AE283A (ABR Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   ABR DH domain with N282A and E283A mutations

   Promoter

   Available since

   Oct. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pLV_hEF1a_rtTA3

   Plasmid

   #61472

   Purpose

   3rd generation lentiviral vector; constitutive expression of rtTA3 (TetON system)

   Depositor

   Ron Weiss

   Article

   Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

   Insert

   rtTA3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hEF1a

   Available since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLV_TRET_hNgn1_UBC_Bla

    Plasmid

    #61473

    Purpose

    3rd generation lentiviral vector; TetON promoter driving human Ngn1; constitutive expression of Blasticidine selection marker

    Depositor

    Ron Weiss

    Article

    Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

    Inserts

    Ngn1 (NEUROG1 Human)
    Bla
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLV_TRET_hNgn2_UBC_Puro

    Plasmid

    #61474

    Purpose

    3rd generation lentiviral vector; TetON promoter driving human Ngn2; constitutive expression of Puromycin selection marker

    Depositor

    Ron Weiss

    Article

    Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

    Inserts

    Ngn2 (NEUROG2 Human)
    Puro
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLV_TRET_Ngn2‐2A‐Ngn1

    Plasmid

    #61471

    Purpose

    3rd generation lentiviral vector; TetON promoter driving mouse Ngn2 and Ngn1

    Depositor

    Ron Weiss

    Article

    Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

    Inserts

    Ngn2 (Neurog2 Mouse)
    Neurog1 (Neurog1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pNF579

    Plasmid

    #26616

    Depositor

    Nancy Freitag

    Article

    Drevets et al Infect Immun. 2001 Mar . 69(3):1344-50.

    Insert

    gfpuv
    

    Use

    Tags

    gram-positive ribosome binding site

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pCSaE500

    Plasmid

    #53182

    Purpose

    Inducible expression of E gene which produces a lysis protein that induces autolysis.

    Depositor

    Lingchong You

    Article

    Tanouchi et al Mol Syst Biol. 2012;8:626. doi: 10.1038/

    Insert

    pCSaE500
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    ampR-PampC cassette

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pNN_v2

    Plasmid

    #32182

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    TALE Monomer NN
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHD_v2

    Plasmid

    #32183

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    TALE Monomer HD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pNG_v2

    Plasmid

    #32181

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    TALE Monomer NG
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNI_v2

    Plasmid

    #32180

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    TALE Monomer NI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTALEN_v2 (NG)

    Plasmid

    #32190

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    TALE Nuclease, NG for 0.5 repeat
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTALEN_v2 (HD)

    Plasmid

    #32192

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    TALE Nuclease, HD for 0.5 repeat
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTALEN_v2 (NI)

    Plasmid

    #32189

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    TALE Nuclease, NI for 0.5 repeat
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTALETF_v2 (NN)

    Plasmid

    #32185

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    TALE Transcriptional Activator Backbone, NN for 0.5 repeat
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTALEN_v2 (NN)

    Plasmid

    #32191

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    TALE Nuclease, NN for 0.5 repeat
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTALETF_v2 (HD)

    Plasmid

    #32188

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    TALE Transcriptional Activator Backbone, HD for 0.5 repeat
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTALETF_v2 (NG)

    Plasmid

    #32186

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    TALE Transcriptional Activator Backbone, NG for 0.5 repeat
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTALETF_v2 (NI)

    Plasmid

    #32187

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    TALE Transcriptional Activator Backbone, NI for 0.5 repeat
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pNB687

    Plasmid

    #60119

    Purpose

    Yellow beetle luciferase

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    YeLuc
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PX851

    Plasmid

    #62883

    Purpose

    N-term SpCas9 piece of inducible split-4 (Cas9(N)-FRB-NES split-4).

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa 2-535)
    

    Use

    CRISPR

    Tags

    FRB and NES PTK2

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    March 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. hAAVS1 1R TALEN

    Plasmid

    #35432

    Purpose

    TALEN designed to target the human AAVS1 locus

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    hAAVS1 1R TALEN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. hAAVS1 1L TALEN

    Plasmid

    #35431

    Purpose

    TALEN designed to target the human AAVS1 locus

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    hAAVS1 1L TALEN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge