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We narrowed to 1,015 results for: HTA

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Showing: 881 - 920 of 1015 results

  3. TDP-REGv2(mScarlet-FLAG)#7 in pTwist-CMV backbone

    Plasmid
    #216152
    Purpose
    Expresses mScarlet in cells with TDP-43 loss-of-function. This plasmid has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells. It uses a cryptic exon. [Code 'A11'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mScarlet with cryptic exon and C-terminal FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216152-plasmid-map-sequence-id-437762

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  5. TDP-REGv2(mScarlet-FLAG)#9 in pTwist-CMV backbone

    Plasmid
    #216153
    Purpose
    Expresses mScarlet in cells with TDP-43 loss-of-function. Has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells (brighter than #7). It uses a single intron. [Code 'B11']. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mScarlet with cryptic splice site and C-terminal FLAG tag
    Expression
    Mammalian
    Available Since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216153-plasmid-map-sequence-id-437757

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  6. AP1muA-mStayGold-polyA-Hygro HR

    Plasmid
    #229679
    Purpose
    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with monomeric StayGold. Contains a hygromycin resistance cassette for selection of edited cells
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence AP1M1 locus (AP1M1 Human)
    Use
    CRISPR
    Tags
    monomeric StayGold
    Available Since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229679-plasmid-map-sequence-id-457128

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  7. AP1muA-SNAP-V5-polyA-Puro HR

    Plasmid
    #229680
    Purpose
    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with SNAP tag and a V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells.
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence AP1M1 locus (AP1M1 Human)
    Use
    CRISPR
    Tags
    SNAP, V5
    Available Since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229680-plasmid-map-sequence-id-457075

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  8. loxP-G418-loxP-3xALFA-Halo-Rab11 HR

    Plasmid
    #229676
    Purpose
    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with Halotag and 3x ALFA tag. Contains a G418 resistance cassette for selection of edited cells
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence Rab11 locus (RAB11A Human)
    Use
    CRISPR
    Tags
    Halo, 3xALFA
    Available Since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229676-plasmid-map-sequence-id-457076

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  9. loxP-Puro-loxP-3xV5-SNAP-Rab11 HR

    Plasmid
    #229677
    Purpose
    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with SNAP tag and a 3x V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells.
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence Rab11 locus (RAB11A Human)
    Use
    CRISPR
    Tags
    SNAP, 3xV5
    Available Since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229677-plasmid-map-sequence-id-457129

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  15. TDP-REGv2 Triple Cryptic Cre recombinase in pTwist-CMV

    Plasmid
    #216162
    Purpose
    Expresses Cre recombinase in cells lacking TDP-43 function. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    Cre recombinase with N-terminal SV40 NLS and C-terminal c-Myc NLS
    Use
    Cre/Lox
    Expression
    Mammalian
    Available Since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216162-plasmid-map-sequence-id-437755

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  35. TDP-REGv2(mScarlet-FLAG)#11 in pTwist-CMV backbone

    Plasmid
    #216155
    Purpose
    Expresses mScarlet in cells with TDP-43 loss-of-function. Very bright and decent dynamic range (>10-fold), slightly leaky. It uses a single intron. [Code 'B12']. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mScarlet with cryptic splice site and C-terminal FLAG tag
    Expression
    Mammalian
    Available Since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216155-plasmid-map-sequence-id-437763

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  37. pAAV-STAT-CA

    Plasmid
    #195576
    Purpose
    Expresses STAT3 constitutively active mutant
    Depositor
    Kevin Park
    Article
    Luo et al Cell Rep. 2016 Apr 12;15(2):398-410. doi
    Insert
    Signal transducer and activator of transcription 3 (Stat3 Mouse)
    Use
    AAV
    Expression
    Mammalian
    Mutation
    changed Alanine 662 to Cysteine and Asparagine 66…
    Promoter
    CMV enhancer and promoter
    Available Since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195576-plasmid-map-sequence-id-383906

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  39. pAAV hSyn syph-GFP-myc-BoNT/B(1-146)-iLID

    Plasmid
    #122981
    Purpose
    AAV plasmid with human synapsin promoter driving synaptophysin fused to GFP, iLID(V416I) and BoNT/B amino acids 1-146. Co-express with SSPB-BoNT(147-441, Y365A) for vPA-BoNT
    Depositor
    Matthew Kennedy
    Article
    Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)
    Insert
    Syph-GFP-myc-BoNT/B(1-146)-iLID (Syp Synthetic, Rat)
    Use
    AAV
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    human synapsin
    Available Since
    March 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    122981-plasmid-map-sequence-id-238426

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Showing: 881 - 920 of 1015 results