We narrowed to 1,037 results for: David Sabatini

1. beta-catenin (S33Y)-pcw107

   Plasmid

   #64615

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   Kris Wood, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   CTNNB1 (CTNNB1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   S33Y

   Promoter

   PGK

   Available since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. beta-catenin (S33A, S37A, T41A, S45A)-pcw107-V5

   Plasmid

   #64613

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   Kris Wood, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   Ctnnb1 (Ctnnb1 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   S33A, S37A, T41A, S45A

   Promoter

   PGK

   Available since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. SmoM2 (W535L)-pcw107

   Plasmid

   #64627

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   Kris Wood, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   SMO (SMO Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   W535L

   Promoter

   PGK

   Available since

   May 15, 2015

   Availability

   Academic Institutions and Nonprofits only

4. FLAG-IKKbeta (S177E,S181E)-pcw107

   Plasmid

   #64609

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   Kris Wood, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   IKBKB
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   S177E, S181E

   Promoter

   PGK

   Available since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

5. FLAG-Rheb (Q64L)-pcw107

   Plasmid

   #64607

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   Kris Wood, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   RHEB (RHEB Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Q64L

   Promoter

   PGK

   Available since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

6. [pSK581] pRK5 HA-SLC38A9N

   Plasmid

   #136141

   Purpose

   mammalian expression of SLC38A9 N term fragment (1-119)

   Depositor

   David Sabatini, Kuang Shen

   Article

   Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

   Insert

   SLC38A9 N term fragment (1-119) (SLC38A9 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. [pSK131] pCOLADuet p14|MP1

   Plasmid

   #136145

   Purpose

   bacterial expression of p14 and MP1

   Depositor

   David Sabatini, Kuang Shen

   Article

   Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

   Insert

   p14 and MP1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcw107

   Plasmid

   #62511

   Purpose

   Gateway Expression Vector

   Depositor

   John Doench, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Jan. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p53 (dominant negative R175H mutant)-pcw107-V5

   Plasmid

   #64638

   Purpose

   3rd generation lentiviral transfer plasmid. When used to produce lentivirus, express physiological levels of insert

   Depositor

   Kris Wood, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   TP53 (transcript variant 3) (TP53 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   R175H

   Promoter

   PGK

   Available since

   Dec. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. [pSK122] pETDuet GST-p18(delta5)

    Plasmid

    #136144

    Purpose

    bacterial expression of GST-p18

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

    Insert

    GST-p18
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. [pSK24] pACYCDuet His-c7orf59|HBXIP

    Plasmid

    #136143

    Purpose

    bacterial expression of c7orf59 and HBXIP

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

    Insert

    c7orf59 and HBXIP (LAMTOR4, LAMTOR5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRK5 HA-RagA(Q66L)

    Plasmid

    #99712

    Purpose

    Mammalian expression of HA-RagA(Q66L)

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Insert

    RagA (RRAGA )
    

    Use

    Tags

    HA

    Expression

    Mutation

    Q66L

    Promoter

    CMV

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCW57.1 tTA UQCRC2

    Plasmid

    #177978

    Purpose

    All-in-one dox-repressible expression of cDNA

    Depositor

    David Sabatini, Jessica Spinelli

    Article

    Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

    Insert

    UQCRC2 (UQCRC2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Silent point mutations to UQCRC2 sgRNA target

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCW57.1 tTA SDHB

    Plasmid

    #177977

    Purpose

    All-in-one dox-repressible expression of cDNA

    Depositor

    David Sabatini, Jessica Spinelli

    Article

    Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

    Insert

    SDHB (SDHB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Silent point mutations to SDHB sgRNA target

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FLAG-Mkk7-JNK2 fusion-pcw107

    Plasmid

    #64618

    Purpose

    Mkk7-JNK2 fusion - barcoded activating construct of JNK pathway

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    Map2k7 linker MAPK9
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    mouse-human fusion

    Promoter

    PGK

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pDarmo.CMVT_v1

    Plasmid

    #133072

    Purpose

    Entry vector for cloning genes under an enhanced CMVT promoter for expression in mammalian cells. Version 1.0.

    Depositor

    Kacper Rogala, David Sabatini

    Article

    Rogala et al Science. 2019 Oct 25;366(6464):468-475.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    3C-Flag-TwinStrep and TwinStrep-Flag-3C

    Expression

    Mammalian

    Mutation

    Promoter

    CMVT (Enhanced CMV)

    Available since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFloat.cspA_v1

    Plasmid

    #133071

    Purpose

    Entry vector for cloning genes under a cold-shock cspA promoter for expression in bacteria. Version 1.0.

    Depositor

    Kacper Rogala, David Sabatini

    Article

    Rogala et al Science. 2019 Oct 25;366(6464):468-475.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    3C-His6 and His6-3C

    Expression

    Bacterial

    Mutation

    Promoter

    cspA

    Available since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRK5 HA-Nprl2

    Plasmid

    #99709

    Purpose

    Mammalian expression of HA-Nprl2

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Insert

    Nprl2 (NPRL2 )
    

    Use

    Tags

    HA

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pETDuet RagA-RagC

    Plasmid

    #99641

    Purpose

    Bacterial expression of wildtype Rag GTPase dimer

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. myr-FLAG-AKT1-pcw107

    Plasmid

    #64606

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and myristoylated

    Expression

    Mutation

    Promoter

    PGK

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only