We narrowed to 581 results for: Nut

1. pLV-UbC-Tet1v4-dCas9

   Plasmid

   #232180

   Purpose

   Tet1v4-dCas9 from Nuñez et al. 2021, in a lentiviral cassette with blasticidin resistance, for expression in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

   Insert

   Tet1 catalytic domain - XTEN80 linker - dead Cas9 - 2A - BlastR (TET1 Human, S. pyogenes)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   hUbC

   Available since

   March 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-UbC-Tet1c-dCas9

   Plasmid

   #232178

   Purpose

   Lentiviral cassette that expresses Tet1c-dCas9 fusion with a short linker in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

   Insert

   Tet1 catalytic domain - dead Cas9 - 2A - BlastR (TET1 Human, S. pyogenes)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   hUbC

   Available since

   March 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. K-to-R mutant NPRL3(1-569) in pLJM60, codon-optimized

   Plasmid

   #184563

   Purpose

   CMV-driven expression of an untagged mutant NPRL3 (core subunit of GATOR1) — codon-optimized sequence for stable expression in mammalian cells. All lysine residues were mutated to arginines.

   Depositor

   Kacper Rogala

   Article

   Valenstein et al Nature. 2022 Jul;607(7919):610-616. doi:

   Insert

   C16orf35, CGTHBA, FFEVF3, HS-40, MARE, NPR3, RMD11 (NPRL3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   All lysines were mutated to arginines. Codon-opti…

   Promoter

   CMV

   Available since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBSWHhc-Lam

   Plasmid

   #133902

   Purpose

   Negative control when used in combination with pAWH-largeT. Expression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWH

   Depositor

   Sebastian Maurer

   Article

   Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

   Insert

   LMNA (Lam) (LMNA Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   T7

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTRIP TRE BI_SEPT2-14-GFP11_SEPT2-14-GFP10

   Plasmid

   #180350

   Purpose

   mammalian co-expression of human SEPT2 fused to GFP10 and of human SEPT2 fused to GFP11

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Inserts

   SEPTIN2 (SEPTIN2 Human)
   SEPTIN 2 (SEPTIN2 Human)
   IRES-TagBFP
   

   Use

   Lentiviral

   Tags

   GFP10 and GFP11

   Expression

   Mammalian

   Mutation

   Promoter

   bidirectional TRE

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTRIP TRE BI_SEPT9_i1-14-GFP11_SEPT9_i1-14-GFP10

   Plasmid

   #180351

   Purpose

   mammalian co-expression of human SEPT9_i1 fused to GFP10 and of human SEPT9_i1 fused to GFP11

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Inserts

   SEPTIN9_v1 (SEPTIN9 Human)
   SEPTIN9_v1 (SEPTIN9 Human)
   IRES-TagBFP
   

   Use

   Lentiviral

   Tags

   GFP10 and GFP11

   Expression

   Mammalian

   Mutation

   Promoter

   bidirectional TRE

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTRIP TRE BI_SEPT9_i3-14-GFP11_SEPT9_i3-14-GFP10

   Plasmid

   #180352

   Purpose

   mammalian co-expression of human SEPT9_i3 fused to GFP10 and of human SEPT9_i3 fused to GFP11

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Inserts

   SEPTIN9_v3 (SEPTIN9 Human)
   SEPTIN9_v3 (SEPTIN9 Human)
   IRES-TagBFP
   

   Use

   Lentiviral

   Tags

   GFP10 and GFP11

   Expression

   Mammalian

   Mutation

   Promoter

   bidirectional TRE

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTRIP TRE BI_SEPT2 NCmut-14-GFP11_SEPT2 NCmut-14-GFP10

   Plasmid

   #180357

   Purpose

   mammalian co-expression of human SEPT2 NCmut fused to GFP10 and of human SEPT2 NCmut fused to GFP11

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Inserts

   SEPTIN2 (SEPTIN2 Human)
   SEPTIN 2 (SEPTIN2 Human)
   IRES-TagBFP
   

   Use

   Lentiviral

   Tags

   GFP10 and GFP11

   Expression

   Mammalian

   Mutation

   SEPT2 F20D, V27D

   Promoter

   bidirectional TRE

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTRIP TRE BI_SEPT9_i1 NCmut-14-GFP11_SEPT9_i1 NCmut-14-GFP10

   Plasmid

   #180358

   Purpose

   mammalian co-expression of human SEPT9_i1 NCmut fused to GFP10 and of human SEPT9_i1 NCmut fused to GFP11

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Inserts

   SEPTIN9_v1 (SEPTIN9 Human)
   SEPTIN9_v1 (SEPTIN9 Human)
   IRES-TagBFP
   

   Use

   Lentiviral

   Tags

   GFP10 and GFP11

   Expression

   Mammalian

   Mutation

   SEPT9_i1 I281D, M288D

   Promoter

   bidirectional TRE

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTRIP TRE BI_SEPT9_i3 NCmut-14-GFP11_SEPT9_i3 NCmut-14-GFP10

    Plasmid

    #180360

    Purpose

    mammalian co-expression of human SEPT9_i3 NCmut fused to GFP10 and of human SEPT9_i3 NCmut fused to GFP11

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Inserts

    SEPTIN9_v3 (SEPTIN9 Human)
    SEPTIN9_v3 (SEPTIN9 Human)
    IRES-TagBFP
    

    Use

    Lentiviral

    Tags

    GFP10 and GFP11

    Expression

    Mammalian

    Mutation

    SEPT9_i3 I263D, M270D

    Promoter

    bidirectional TRE

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTKA001

    Plasmid

    #164577

    Purpose

    Expresses SARS-CoV-2 nsp7 from a pET46 backbone for IPTG-inducible bacterial expression

    Depositor

    Robert Kirchdoerfer

    Article

    Altincekic et al Front Mol Biosci. 2021 May 10;8:653148.

    Insert

    nsp7 (ORF1ab )
    

    Use

    Tags

    6xHis, Ek, TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTKA002

    Plasmid

    #164578

    Purpose

    Expresses SARS-CoV-2 nsp8 from a pET46 backbone for IPTG-inducible bacterial expression

    Depositor

    Robert Kirchdoerfer

    Article

    Altincekic et al Front Mol Biosci. 2021 May 10;8:653148.

    Insert

    nsp8
    

    Use

    Tags

    6xHis, Ek, TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPtPBR11_fcpShble_GWB

    Plasmid

    #154031

    Purpose

    Gateway cloning into P. tricornutum episomal vector

    Depositor

    Raffaela Abbriano

    Type

    Empty backbone

    Use

    Diatom cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. rrsB-RFP

    Plasmid

    #63838

    Purpose

    This plasmid is designed as a bacterial nutrient sensor

    Depositor

    Howard Salis

    Insert

    rrsB, mRFP1 and GFPmut3b
    

    Use

    Tags

    mRFP1

    Expression

    Bacterial

    Mutation

    Gene is transcriptionally fused to RFP

    Promoter

    Available since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. rrsA-RFP

    Plasmid

    #63837

    Purpose

    This plasmid is designed as a bacterial nutrient sensor

    Depositor

    Howard Salis

    Insert

    rrsA, mRFP1 and GFPmut3b
    

    Use

    Tags

    mRFP1

    Expression

    Bacterial

    Mutation

    Gene is transcriptionally fused to RFP

    Promoter

    Available since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBOB-Septin 2 -GFP

    Plasmid

    #118734

    Purpose

    To express Septin 2 tagged with GFP on C-terminus

    Depositor

    John Cooper

    Insert

    Septin 2 (SEPTIN2 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBOB-Septin 2 -tdTomato

    Plasmid

    #118735

    Purpose

    To express Septin 2 tagged with tdTomato on C-terminus

    Depositor

    John Cooper

    Insert

    Septin 2 (SEPTIN2 Human)
    

    Use

    Lentiviral

    Tags

    tdTomato

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET23b-NTF2

    Plasmid

    #108918

    Purpose

    Expresses NTF2

    Depositor

    Larry Gerace

    Article

    Cassany et al Methods Mol Biol. 2009;464:181-205. doi:

    Insert

    NTF2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. 2QNR (NEDD5)

    Plasmid

    #101273

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    NEDD5 (SEPTIN2 Human)
    

    Use

    Tags

    Hexahistidine tag with Thrombin cleavage

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 2, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pDL12

    Plasmid

    #17834

    Depositor

    Jay Keasling

    Article

    Lubertozzi et al Appl Microbiol Biotechnol. 2006 Oct . 72

    Insert

    gpdA promoter, lacZ, argB,
    

    Use

    Synthetic Biology; Aspergillus nidulans expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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