Addgene

We narrowed to 1,051 results for: SEF;

Showing: 441 - 460 of 1051 results

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pJOE6227.1

Plasmid

#135078

Purpose

An integration vector for the chromosomal deletion of the upp gene in P. putida KT2440; carries the upp deletion cassette.

Depositor

Josef Altenbuchner

Article

Graf et al Appl Environ Microbiol. 2011 Aug;77(15):

Insert

up- and downstream regions of upp (upp Pseudomonas putida)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pJOE4905.1

Plasmid

#135085

Purpose

rhamnose-inducible expression vector

Depositor

Josef Altenbuchner

Article

Motejadded et al Biotechnol Lett. 2009 Apr;31(4):543-9. d

Insert

P-rhaP-BAD-malE-His tag-smt3-eGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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SEAP (Protein secretion reporter) in pLX317

Plasmid

#233290

Purpose

SEAP reporter (used for monitoring protein secretion)

Depositor

Sefi Rosenbluh

Insert

SEAP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

E1Fa

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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inducible shSRP19_9 in pLKO tet on

Plasmid

#233289

Purpose

Dox inducible expression of SRP19 shRNA

Depositor

Sefi Rosenbluh

Insert

shSRP19

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Dox

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-Myo19

Plasmid

#127612

Purpose

GFP-tagged Myosin-19

Depositor

Josef Kittler

Article

Lopez-Domenech et al EMBO J. 2018 Feb 1;37(3):321-336. doi: 1

Insert

Myo19

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pJH181.3

Plasmid

#135076

Purpose

Coryne. glutamicum HA production plasmid

Depositor

Josef Altenbuchner

Article

Hoffmann et al J Appl Microbiol. 2014 Sep;117(3):663-78

Insert

hasA-hasC

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pDisplay-mgSrtA

Plasmid

#125792

Purpose

Transfected into cell of interest for membrane expression. The encoded mgSrtA could enable labeling of the interacting cells which is useful for recording cell-cell interactions.

Depositor

P. Chen

Article

Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

Insert

mgSrtA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-eGFP-C1-VPS35

Plasmid

#163622

Purpose

human VPS35 cDNA C-terminally tagged with eGFP

Depositor

Josef Kittler

Article

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Insert

human VPS35 (VPS35 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pJeM1

Plasmid

#135088

Purpose

rhamnose-inducible expression vector for replication in E. coli and P. putida

Depositor

Josef Altenbuchner

Article

Jeske et al Appl Microbiol Biotechnol. 2010 Feb;85(6

Insert

rhaR rhaS rhaPBAD eGFP, mob (rhaR E.coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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AiP11839-pAAV-hSyn1-SYFP2-10aa-H2B-WPRE3-BGHpA (Alias: CN1839)

Plasmid

#163509

Purpose

AAV vector for nuclear SYFP2 labeling in neurons. Useful for nuclear isolation and scRNA-seq applications.

Depositor

Jonathan Ting, The Allen Institute for Brain Science

Has Service

AAV PHP.eB

Insert

H2B-SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn1

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-mSNX27

Plasmid

#163617

Purpose

mouse SNX27 cDNA N-terminally tagged with GFP

Depositor

Josef Kittler

Article

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Insert

mouse SNX27 (Snx27 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pJOE7771.1

Plasmid

#135077

Purpose

Mannitol, arabitol, and glucitol-inducible mtlR/PmtlE expression vector

Depositor

Josef Altenbuchner

Article

Hoffmann et al PLoS One. 2015 Jul 24;10(7):e0133248. do

Insert

mtlR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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myc-Pex19

Plasmid

#127625

Purpose

N-terminal myc-tagged mouse Pex19

Depositor

Josef Kittler

Article

Covill-Cooke et al EMBO Rep. 2020 Feb 5;21(2):e49865. doi:

Insert

Pex19 (Pex19 Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pEFIRES NQO1 WT

Plasmid

#61735

Purpose

Expresses WT human NQO1

Depositor

Yosef Shaul

Article

Asher et al Genes Dev. 2005 Feb 1;19(3):316-21.

Insert

NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)

Use

Tags

None

Expression

Mammalian

Mutation

wild type

Promoter

EF1a

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-mSNX27-ΔF3

Plasmid

#163618

Purpose

mouse SNX27 without the F3 domain cDNA C-terminally tagged with GFP

Depositor

Josef Kittler

Article

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Insert

mouse SNX27-ΔF3 (Snx27 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

deletion F3 domain

Promoter

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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SyGCaMP5G

Plasmid

#162519

Purpose

Synaptically targeted GCaMP5G

Depositor

Josef Kittler

Article

Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

Insert

GCaMP5G

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-mSNX27-H112A

Plasmid

#163619

Purpose

mouse SNX27 cDNA with a point mutation H112A C-terminally tagged with GFP

Depositor

Josef Kittler

Article

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Insert

mouse SNX27-H112A (Snx27 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

H112A

Promoter

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEFIRES NQO1 Y128F

Plasmid

#61734

Purpose

Expresses human Y128F NQO1

Depositor

Yosef Shaul

Article

Asher et al Genes Dev. 2005 Feb 1;19(3):316-21.

Insert

NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)

Use

Tags

None

Expression

Mammalian

Mutation

Y128F

Promoter

Ef1a

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pBS7

Plasmid

#83774

Purpose

Useful for tagging yeast proteins with C-terminal Venus tag; G418 selection

Depositor

Eric Muller

Article

Hailey et al Methods Enzymol. 2002;351:34-49.

Insert

Venus

Use

Tags

Venus

Expression

Yeast

Mutation

F46L, F64L, S65G, V68L, Q69M, S72A, Q80R, M153T, …

Promoter

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pEFIRES NQO1 Y128V

Plasmid

#61733

Purpose

Expresses human Y128V NQO1

Depositor

Yosef Shaul

Article

Asher et al Genes Dev. 2005 Feb 1;19(3):316-21.

Insert

NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)

Use

Tags

None

Expression

Mammalian

Mutation

Y128V

Promoter

Ef1a

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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