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Plasmid#209875PurposeDimerization dependent fluorescent protein RA anchored to cytosolic face of the lysosomal membrane with human LAMP1DepositorInsertddRFP A
UseTagsLAMP1ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
R57C10-Flp2
Plasmid#63172PurposeR57C10 (N-synaptobrevin), "Pan-Neuronally," driven wild type yeast Flp2 promoter-fusionDepositorInsertyeast wild type containing a Glycine at amino acid 5 of the protein sequence
UseTagsExpressionInsectMutationPromoterAvailable sinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
MmSphk1
Plasmid#118599PurposeExpresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate in miceDepositorInsertMus musculus sphingosine kinase 1
UseTagsFLAGExpressionYeastMutationPromoterGAL1, 10Available sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
HsSPHK1
Plasmid#118091PurposeExpresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)DepositorInsertHomo sapiens Sphingosine Kinase 1
UseTagsFLAGExpressionYeastMutationPromoterGAL1, 10Available sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV-Halo-10-PFN1
Plasmid#204222PurposeExpression of Halo-Profilin-1 in tissue culture cellsDepositorInsertPFN1 (PFN1 Human)
UseTagsHalo, TEV site, SGLRSRAQAS linkerExpressionMammalianMutationPromoterCMVAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
AMPK-SPARK
Plasmid#226255Purposephases-of-separation based activity reporter of AMPKDepositorInsertAMPK-SPARK
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
Yellow Cameleon-Nano15/pcDNA3
Plasmid#51961Purposemammalian expression of Yellow Cameleon-Nano15, an ultrasensitive Ca2+ indicator with Kd = 15 nMDepositorInsertYellow Cameleon-Nano15
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
PM-RA
Plasmid#209878PurposeDimerization dependent fluorescent protein RA anchored to cytosolic face of the plasma membrane with the N-terminal targeting sequence of human GAP43DepositorInsertddRFP A
UseTagsTargeting domain of Gap43 MLCCMRRTKQVEKNDDQKIExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 iTEV
Plasmid#64277PurposeExpresses infrared fluorescent tobacco etch virus protease sensor (iTEV)DepositorInsertInfrared fluorescent tobacco etch virus protease sensor (iTEV)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-) FLIPQTV3.0 100micro
Plasmid#63730PurposeFLIPQTV3.0 glutamine sensor with D157N mutationDepositorInsertFLIPQTV3.0 sensor, 100microM affinity
UseTagsExpressionMammalianMutationD157N in glutamine binding domainPromoterAvailable sinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_VP35_MARV
Plasmid#135471PurposeEncodes Marburg virus VP35 gene under control of the AG promoterDepositorInsertVP35
UseTagsExpressionMammalianMutationNonePromoterAGAvailable sinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_NP_MARV
Plasmid#135470PurposeEncoding Marburg virus NP gene under control of the AG promoterDepositorInsertnucleoprotein (NP)
UseTagsExpressionMammalianMutationNonePromoterAGAvailable sinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
#1.4 pre-G-SynapShot-TagBFP
Plasmid#214717PurposeExpresses green #1.4 pre-SynapShot and TagBFPDepositorInsert#1.4 pre-G-SynapShot
UseAAVTagsTagBFPExpressionMammalianMutationPromoterCAGAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPS0377
Plasmid#115509PurposeReporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing sfGFPDepositorInsertsfGFP (pOGG037) and T-pharma (pOGG003) assembled in pOGG026
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterConstitutive promoter pNeo pOGG001Available sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSB-CMV-MCS-Puro GEM-LTB4mut
Plasmid#202642PurposeExpresses the LTB4-insensitive control sensor GEM-LTB4mut in mammalian cellsDepositorInsertGEM-LTB4mut
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP mCherry-ApoB-EGFP
Plasmid#112859PurposePlasmid bearing the editing target of APOBEC1 fused between the mCherry cds and the EGFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of EGFPDepositorInsertmCherry-apoB-EGFP
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cav-GA
Plasmid#209880PurposeDimerization dependent fluorescent protein GA anchored to cytosolic face of the caveolae with mouse CAVIN1DepositorInsertddGFP A
UseTagsCAVIN1ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav-RA
Plasmid#209881PurposeDimerization dependent fluorescent protein RA anchored to cytosolic face of the caveolae with mouse CAVIN1DepositorInsertddRFP A
UseTagsCAVIN1ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
1/8-NORAD-(A)4x
Plasmid#199209Purpose1/8 truncation of NORAD mRNA tagged with 4 copies of Riboglow (variant A)DepositorInsert1/8-NORAD
UseTagsRiboglow RNA tagExpressionMammalianMutation4 copies of Riboglow tag after stop codonPromoterCMVAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
1/2-NORAD-(A)4x
Plasmid#199208Purpose1/2 truncation of NORAD mRNA tagged with 4 copies of Riboglow (variant A)DepositorInsert1/2-NORAD
UseTagsRiboglow RNA tagExpressionMammalianMutation4 copies of Riboglow tag after stop codonPromoterCMVAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only