We narrowed to 2,617 results for: pCas

Showing: 441 - 460 of 2617 results

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pCas9-EGFP_SLEEK

Plasmid

#249545

Purpose

Insert the Cas9-EGFP into exon 9 of GAPDH for stable expression.

Depositor

Joe Zhang

Publication

Zhang et al STAR Protoc. 2025 Jul 4;6(3):103928. doi

Insert

5'arm-Exon9-Cas9-EGFP-3'arm

Use

CRISPR

Tags

EGFP

Promoter

U6 promoter

Available since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pSL2621 (pCas8_Vch)

Plasmid

#200893

Purpose

Expresses human codon-optimized V. cholerae Cas8 from a CMV promoter.

Depositor

Samuel H. Sternberg

Publication

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

VchCas8

Tags

NLS

Expression

Mammalian

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pSL2623 (pCas6_Vch)

Plasmid

#200895

Purpose

Expresses human codon-optimized V. cholerae Cas6 from a CMV promoter.

Depositor

Samuel H. Sternberg

Publication

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

VchCas6

Tags

NLS

Expression

Mammalian

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pX459_gRNA-ROSA26_hspCas9

Plasmid

#193310

Purpose

Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)

Depositor

Simon Elsaesser

Publication

Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)

Use

CRISPR

Expression

Mammalian

Mutation

not applicable

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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SpCas9 TadCBEe

Plasmid

#193836

Purpose

Express TadCBEe (with SpCas9) in mammalian cells

Depositor

David Liu

Publication

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TadA-CDe-SpCas9D10A-2xUGI

Expression

Mammalian

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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p2T-CAG-SpCas9-BlastR

Plasmid

#107190

Purpose

Confers constitutive expression of SpCas9

Depositor

Richard Sherwood

Publication

Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SpCas9 (NEWENTRY )

Use

CRISPR

Tags

3xFLAG

Expression

Bacterial and Mammalian

Promoter

Chicken ß-actin

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Pb-TRE3G-SpCas9-IRES-blast

Plasmid

#195506

Purpose

Inducible Piggybac vector expressing SpCas9 with Blasticidin selection

Depositor

Joanna Wysocka

Publication

Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

spCas9

Use

CRISPR

Expression

Mammalian

Promoter

TRE

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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SpCas9-CBE6c-IVT

Plasmid

#215835

Purpose

Template for in vitro transcription of CBE6c (with SpCas9)

Depositor

David Liu

Publication

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6c-SpCas9-2xUGI

Use

In vitro transcription template

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6a-V106W

Plasmid

#215824

Purpose

Express CBE6a-V106W (with SpCas9) in mammalian cells

Depositor

David Liu

Publication

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CBE6a V106W-SpCas9-2xUGI (cas9 )

Use

In vitro transcription template

Available since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCAS9-mCherry-HIST1H4C

Plasmid

#66944

Purpose

CRISPaint target selector HIST1H4C

Depositor

Veit Hornung

Publication

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA HIST1H4C

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCaSpeR-act5cB-QF#7m1

Plasmid

#46126

Depositor

Christopher Potter

Publication

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QF#7m1

Expression

Insect

Available since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCaSpeR4-tubulin-QF#7m1

Plasmid

#46128

Depositor

Christopher Potter

Publication

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QF#7m1

Expression

Insect

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pCAGEN-dPspCas13b-NES-mCherry

Plasmid

#233609

Purpose

Expression of catalytically-inactive PspCas13b in mammalian cells

Depositor

Shintaro Iwasaki

Publication

Apostolopoulos et al Nat Commun. 2024 Mar 11;15(1):2205. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dPspCas13b-NES-mCherry

Use

CRISPR

Tags

3xFlag and mCherry

Mutation

H133A/H1058A

Available since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCashGem-Xyl2-URA3-LINEAR

Plasmid

#174840

Purpose

pRS414 backbone containing LINEAR fragment targeting XYL2 in S. stipitis

Depositor

Zengyi Shao

Publication

Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

Insert

Codon optimized Cas9:hGem

Use

CRISPR and Synthetic Biology

Tags

hGeminin tag

Expression

Bacterial and Yeast

Promoter

ENO1p

Available since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Cmv d0 dPspCas13b-GGS-Sun

Plasmid

#119858

Purpose

Cmv d0 dPspCas13b-GGS-Sun no-fusion construct

Depositor

Bryan Dickinson

Publication

Rauch et al J Am Chem Soc. 2018 Sep 26;140(38):11974

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas13b-GGS-Sun

Use

CRISPR

Expression

Mammalian

Available since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pSL2622 (pCas7_Vch)

Plasmid

#200894

Purpose

Expresses human codon-optimized V. cholerae Cas7 from a CMV promoter.

Depositor

Samuel H. Sternberg

Publication

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

VchCas7

Tags

NLS

Expression

Mammalian

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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SpCas9 iFE

Plasmid

#190138

Purpose

Expresses SpCas9-POLB (iFE)

Depositor

Timothy Lu

Publication

Nakade et al bioRxiv 2022.12.05.518807

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SpCas9-POLB (HiFi iFE)

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Codon Optimized for mammalian cells

Promoter

CBh

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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SpCas9 TadCBEb

Plasmid

#193832

Purpose

Express TadCBEb (with SpCas9) in mammalian cells

Depositor

David Liu

Publication

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TadA-CDb-SpCas9 D10A-2xUGI

Expression

Mammalian

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pSPCas9(BB)-2A-GFP_ABCA3GFP_targeting

Plasmid

#188541

Purpose

Plasmid encoding pCas9 and gRNA targeting the endogenous human ABCA3 locus stop codon

Depositor

Darrell Kotton

Publication

Sun et al Am J Respir Cell Mol Biol. 2021 Oct;65(4

Insert

gRNA

Use

CRISPR

Tags

GFP

Promoter

U6

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1a-nSpCas9

Plasmid

#171691

Purpose

Expresses nSpCas9 in mammalian cells

Depositor

Jia Chen

Publication

Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

nSpCas9

Use

CRISPR

Expression

Mammalian

Mutation

D10A

Promoter

EF1a

Available since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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