4,182 results

1. pET21b(+)-Is-PETase-W159H-S238F

   Plasmid

   #112203

   Purpose

   pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W159H and S238F mutations, codon optimized for expression in E. coli K12

   Depositor

   Gregg Beckham, Christopher Johnson

   Article

   Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

   Insert

   PETase gene from Ideonella sakaiensis 201-F6 with W159H and S238F mutations, codon optimized for expression in E. coli K12
   

   Tags

   6XHIS

   Expression

   Bacterial

   Mutation

   W159H, S238F

   Promoter

   N/A

   Available Since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCoPURO

   Plasmid

   #17533

   Depositor

   Francis Castellino

   Article

   Iwaki et al Biotechniques. 2003 Sep . 35(3):482-4, 4

   Insert

   Puromycine Acetyl Transferase
   

   Expression

   Insect

   Available Since

   March 21, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.0 FLAG TRPV4

   Plasmid

   #45751

   Depositor

   Robert Lefkowitz

   Article

   Shukla et al J Biol Chem. 2010 Sep 24;285(39):30115-2

   Insert

   TRPV4 (Trpv4 Rat)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK5-HA-Ubiquitin-K29

   Plasmid

   #22903

   Depositor

   Sandra Weller

   Article

   Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

   Insert

   Ubiquitin C (UBC Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K29 only, all other lysines mutated to arginines

   Available Since

   Jan. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV6-Bmal-luc

   Plasmid

   #68833

   Purpose

   Expression of Bmal1-luc in mammalian cells

   Depositor

   Steven Brown

   Insert

   Bmal1 regulatory sequences (Bmal1 Mouse)
   

   Use

   Lentiviral and Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Available Since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-CMV-CFP;UBC-Cre25nt

   Plasmid

   #65727

   Purpose

   Expresses Cre recombinase with a 25 nucleotide extracellular vesicle targeting sequence under the UBC promotor and CFP under the CMV promotor.

   Depositor

   Jacco van Rheenen

   Article

   Zomer et al Cell. 2015 May 21;161(5):1046-57. doi: 1

   Inserts

   CMV promoter driving CFP expression
   UBC promoter driving Cre-25nt expression
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   CMV and UBC

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CW3SL-EGFP

   Plasmid

   #61463

   Purpose

   AAV vector with CaMKIIa promoter, EGFP, and W3SL(shorten expression cassette with relatively high expression to maximize expression of larger genes)

   Depositor

   Bong-Kiun Kaang

   Article

   Choi et al Mol Brain. 2014 Mar 11;7:17. doi: 10.118

   Insert

   EGFP
   

   Use

   AAV

   Promoter

   CaMKIIa

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-Mff

   Plasmid

   #49153

   Purpose

   mammalian expression of Mff fused to GFP

   Depositor

   Gia Voeltz

   Article

   Friedman et al Science. 2011 Oct 21;334(6054):358-62. d

   Insert

   Mff (MFF Human)
   

   Tags

   acGFP1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3 p53 WT

   Plasmid

   #69003

   Purpose

   mammalian expression of wild type human p53

   Depositor

   David Meek

   Article

   Loughery et al Nucleic Acids Res. 2014 Jul;42(12):7666-

   Insert

   p53 (TP53 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmEGFP_a_tubulin_C1

    Plasmid

    #21039

    Depositor

    Daniel Gerlich

    Article

    Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

    Insert

    alpha Tubulin (TUBA1B Human)
    

    Tags

    pmEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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