We narrowed to 12,459 results for: 109

1. pLEX307-IBV-3CL

   Plasmid

   #160288

   Purpose

   Allows for constituitive mammalian expression of the IBV 3CL protease via transfection or lentivirus

   Depositor

   Alejandro Chavez

   Article

   Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

   Insert

   3CL Protease
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL3 2 kb prom. and exon 1 fragment CD274

   Plasmid

   #107007

   Purpose

   Modified Luciferase expression vector

   Depositor

   Julian Downward

   Article

   Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

   Insert

   CD274 (CD274 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL4.23 WT 2014 EBOV Delta-Mucin-Like-Domain

   Plasmid

   #86021

   Purpose

   Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

   Depositor

   Jeremy Luban, Pardis Sabeti

   Article

   Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

   Insert

   Makona Ebola Virus Glycoprotein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Lacks mucin-like domain

   Promoter

   CMV IE

   Available since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-PPO-Venus

   Plasmid

   #139502

   Purpose

   Expresses Venus-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.

   Depositor

   Michael Bruchas

   Article

   Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

   Insert

   parapinopsin
   

   Use

   Tags

   Venus tag following 3x Ala linker

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.3−3xFLAG−AGO2(S387A)

   Plasmid

   #220462

   Purpose

   Express S387 phospho-null mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells

   Depositor

   David Bartel

   Article

   Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

   Insert

   Human AGO2 (AGO2 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Serine 387 to alanine

   Promoter

   CMV

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-Venus-173-N-ORAI1

   Plasmid

   #87618

   Purpose

   N-terminal Venus (YFP) fragment fused to full-length ORAI1; for BiFC.

   Depositor

   Jin Zhang

   Article

   Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

   Insert

   Venus173-N-ORAI1 (ORAI1 Synthetic, Human)
   

   Use

   Tags

   6xHis, T7 tag (gene 10 leader), and Xpress(TM) ta…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Strep-KIFC1*-HA

   Plasmid

   #120171

   Purpose

   Expresses a chimera of streptavidin, a KIFC1-based retrograde motor and HA tag

   Depositor

   Juan Bonifacino

   Article

   Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

   Insert

   kinesin family member C1 (KIFC1 Human)
   

   Use

   Tags

   HA and Streptavidin

   Expression

   Mammalian

   Mutation

   Truncated KIFC1: 125-673 aa

   Promoter

   CMV

   Available since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET22b-mEB2-eGFP-6xHis

   Plasmid

   #130982

   Purpose

   Bacterial expression of mouse EB2-GFP-6xHis for protein purification.

   Depositor

   Anne Straube

   Article

   Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

   Insert

   EB2-GFP-6xHis (Mapre2 Mouse)
   

   Use

   Tags

   eGFP-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLEX-FLAG-NRASQ61K-IRES-Puro

   Plasmid

   #120575

   Purpose

   Expresses Flag-N-Ras Q61K fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Q61K

   Promoter

   CMV

   Available since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. HT111_Fsyn_FAS(Cre off)_QuasAr6a_Citrine

    Plasmid

    #178824

    Purpose

    Neuronal expression (Cre-off) of an archaerhodopsin-derived genetically encoded voltage indicator QuasAr6b carrying a Citrine expression tag

    Depositor

    Adam Cohen

    Article

    Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

    Insert

    QuasAr6a_citrine
    

    Use

    Cre/Lox and Lentiviral

    Tags

    citrine

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLEX307-HCoV-NL63-3CL

    Plasmid

    #160286

    Purpose

    Allows for constituitive mammalian expression of the HCoV-NL63 3CL protease via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLEX307-Bat-CoV-HKU9-3CL

    Plasmid

    #160284

    Purpose

    Allows for constituitive mammalian expression of the Bat-CoV-HKU9 3CL protease via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MSCV-NSD3-short

    Plasmid

    #72552

    Purpose

    expresses 3*FLAG tagged human NSD3-short

    Depositor

    Christopher Vakoc

    Article

    Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

    Insert

    NSD3-short (NSD3 Human)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    none

    Promoter

    LTR

    Available since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. px458_2A_GFP_sgRNA_EIF2AK2

    Plasmid

    #106108

    Purpose

    CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting EIF2AK2

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    gRNA targeting EIF2AK2 (EIF2AK2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. mEGFP-N1-MST2

    Plasmid

    #166475

    Purpose

    Expresses fusion of mEGFP and MST2

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    mEGFP, MST2 (STK3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX-4T1-WDR5

    Plasmid

    #59969

    Purpose

    Bacterial Expression GST-WDR5

    Depositor

    Debu Chakravarti

    Article

    Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

    Insert

    WDR5 (WDR5 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

17. tetO-SRF

    Plasmid

    #170704

    Purpose

    doxycycline-inducible overexpression of SRF

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    SRF (SRF Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. CIBN-rTetR

    Plasmid

    #183919

    Purpose

    Optogenetic CIBN localizer domain coupled to reverse Tet repressor; binds tetO sites upon doxycycline addition; CIBN is bound by PHR upon blue light exposure

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    CIBN-rTetR (CIB1 Synthetic, Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFL His-SDS22 / PP1gamma

    Plasmid

    #113511

    Purpose

    Expression of His-SDS22 / PP1gamma dimer in insect cells (Baculovirus system)

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Inserts

    SDS22 (PPP1R7 Human)
    PP1gamma (PPP1CC Human)
    

    Use

    Tags

    6*His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLEX307-SARS-CoV-3CL

    Plasmid

    #160280

    Purpose

    Allows for constituitive mammalian expression of the SARS-CoV 3CL protease via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge