We narrowed to 34,231 results for: CaS

1. pC025 - Cg2Cas13a from Carnobacterium gallinarum DSM 4847

   Plasmid

   #91915

   Purpose

   Expresses Cg2Cas13a for bacterial expression and contains backbone for spacer cloning

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   Cg2Cas13a and guide expression cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. NFATc1-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

   Plasmid

   #75238

   Purpose

   CRISPR/Cas9 NICKASE plasmid against human NFATc1 (1/2)

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against NFATc1 (NFATC1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. NFATc1-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

   Plasmid

   #75239

   Purpose

   CRISPR/Cas9 NICKASE plasmid against human NFATc1 (2/2)

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against NFATc1 (NFATC1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJSC090 - Bacterial expression plasmid for SpCas9 + K855A variant

   Plasmid

   #101213

   Purpose

   Bacterial expression plasmid for SpCas9 + K855A variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant K855A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   K855A

   Promoter

   T7

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLPAR1_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE

   Plasmid

   #101116

   Purpose

   Encodes dCas9(N) fused to LPAR1

   Depositor

   Tudor Fulga

   Article

   Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

   Insert

   LPAR1-dCas9(N)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCSDest2-2XNLS-SpCas9-WT-NLS-3XHA-NLS-TALentry

   Plasmid

   #69232

   Purpose

   Wild type SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. nAPEX2-V5-zdCas9n-3x-FLAG-GSG-linker-P2A-mNeongreen

   Plasmid

   #241191

   Purpose

   Middle entry vector for mini-Golden subcloning platform; 3x-FLAG; mNeongreen

   Depositor

   Junsu Kang

   Insert

   3x-FLAG; mNeongreen
   

   Use

   Mini-golden

   Tags

   Expression

   Mutation

   TCGC linker

   Promoter

   Available since

   Aug. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. 3x-FLAG-nzdCas9-V5-APEX2n-GSG-linker-P2A-mNeongreen

   Plasmid

   #241190

   Purpose

   Middle entry vector for mini-Golden subcloning platform; V5-APEX2n; mNeongreen

   Depositor

   Junsu Kang

   Insert

   V5-APEX2n; mNeongreen
   

   Use

   Mini-golden

   Tags

   Expression

   Mutation

   TCGC linker

   Promoter

   Available since

   Aug. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE-IRES-puro-NLS-HyperdLbCas12a-NLS-3xFlag

   Plasmid

   #236386

   Purpose

   Overexpression of HyperdLbCas12a in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

   Insert

   HyperdLbCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   D156R, D235R, E292R, D350R

   Promoter

   Available since

   July 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. (942) pAAV EF1a dCasphi-[myc-ZIM3-HA] U6-gStop

    Plasmid

    #195624

    Purpose

    pAAV EF1a plasmid for constitutive expression of dCasphi-ZIM3 CRISPRi construct

    Depositor

    Tonia Rex

    Insert

    dCasphi
    

    Use

    AAV

    Tags

    C-Myc (NLS), HA tag, and ZIM3

    Expression

    Mammalian

    Mutation

    P749A and P753A

    Promoter

    EF1a (Mlu1/EcoR1)

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-T7-TadCBEd-SpCas9(MRKQKC)-P2A-EGFP (AHK574)

    Plasmid

    #223134

    Purpose

    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with MRKQKC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA-CDd-SpCas9(MRKQKC)-2xUGI-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

    Expression

    Mammalian

    Mutation

    TadA-CDd mutations; nSpCas9(D10A/MRKQKC)=D1135M, …

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-T7-TadCBEd-SpCas9(MRKSKC)-P2A-EGFP (AHK446)

    Plasmid

    #223135

    Purpose

    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with MRKSKC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA-CDd-SpCas9(MRKSKC)-2xUGI-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

    Expression

    Mammalian

    Mutation

    TadA-CDd mutations; nSpCas9(D10A/MRKSKC)=D1135M, …

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-T7-TadCBEd-SpCas9(KWRQLC)-P2A-EGFP (BKS1026)

    Plasmid

    #223126

    Purpose

    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with KWRQLC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA-CDd-SpCas9(KWRQLC)-2xUGI-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

    Expression

    Mammalian

    Mutation

    TadA-CDd mutations; nSpCas9(D10A/KWRQLC)=D1135K, …

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-T7-TadCBEd-SpCas9(LWRYEK)-P2A-EGFP (BKS1022)

    Plasmid

    #223128

    Purpose

    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with LWRYEK amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA-CDd-SpCas9(LWRYEK)-2xUGI-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

    Expression

    Mammalian

    Mutation

    TadA-CDd mutations; nSpCas9(D10A/LWRYEK)=D1135L, …

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-T7-TadCBEd-SpCas9(MKRCMV)-P2A-EGFP (BKS1032)

    Plasmid

    #223129

    Purpose

    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with MKRCMV amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA-CDd-SpCas9(MKRCMV)-2xUGI-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

    Expression

    Mammalian

    Mutation

    TadA-CDd mutations; nSpCas9(D10A/MKRCMV)=D1135M, …

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-T7-TadCBEd-SpCas9(MKRCKS)-P2A-EGFP (RAS3445)

    Plasmid

    #223130

    Purpose

    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with MKRCKS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA-CDd-SpCas9(MKRCKS)-2xUGI-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

    Expression

    Mammalian

    Mutation

    TadA-CDd mutations; nSpCas9(D10A/MKRCKS)=D1135M, …

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-T7-TadCBEd-SpCas9(ARGIMR)-P2A-EGFP (BKS1036)

    Plasmid

    #223132

    Purpose

    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with ARGIMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA-CDd-SpCas9(ARGIMR)-2xUGI-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

    Expression

    Mammalian

    Mutation

    TadA-CDd mutations; nSpCas9(D10A/ARGIMR)=D1135A, …

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-T7-TadCBEd-SpCas9(MRKCRS)-P2A-EGFP (AHK545)

    Plasmid

    #223133

    Purpose

    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with MRKCRS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA-CDd-SpCas9(MRKCRS)-2xUGI-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

    Expression

    Mammalian

    Mutation

    TadA-CDd mutations; nSpCas9(D10A/MRKCRS)=D1135M, …

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-T7-ABE8e-SpCas9(ARGIMR)-P2A-EGFP (AHK507)

    Plasmid

    #223118

    Purpose

    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with ARGIMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA8e-SpCas9(ARGIMR)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-P2A-EGFP and BPNLS-TadA8e

    Expression

    Mammalian

    Mutation

    TadA8e mutations; nSpCas9(D10A/ARGIMR)=D1135A, S1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-T7-ABE8e-SpCas9(LWQYQH)-P2A-EGFP (AHK504)

    Plasmid

    #223119

    Purpose

    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with LWQYQH amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA8e-SpCas9(LWQYQH)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-P2A-EGFP and BPNLS-TadA8e

    Expression

    Mammalian

    Mutation

    TadA8e mutations; nSpCas9(D10A/LWQYQH)=D1135L, S1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge