We narrowed to 4,859 results for: Mela

1. pB[U6-3 wex2 PUb-DsRed]

   Plasmid

   #169010

   Purpose

   For germline transformation. Expresses white exon 2 sgRNA and DsRed marker in all cells.

   Depositor

   Max Scott

   Article

   Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

   Insert

   white ex2 sgRNA
   

   Use

   Piggybac

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Drosophila melanogaster U6:3

   Available Since

   April 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3xFlag-CMV7-Erk2

   Plasmid

   #39223

   Depositor

   Melanie Cobb

   Article

   Robinson et al J Biol Chem. 2002 Apr 26;277(17):14844-5

   Insert

   Erk2 (Mapk1 Rat)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. p3xFlag-CMV7-Erk2_KR

   Plasmid

   #39224

   Depositor

   Melanie Cobb

   Article

   Robinson et al J Biol Chem. 2002 Apr 26;277(17):14844-5

   Insert

   Erk2(K52R) (Mapk1 Rat)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   K52R

   Promoter

   CMV

   Available Since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pJK511

   Plasmid

   #155384

   Purpose

   dCas12a oscillator, reverse direction crRNAs. dCas12a (D917A) + PA4-mVenus

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas12a (F. novicida)
   PA4-mVenus
   dCas12a oscillator crRNAs
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D917A (nuclease-deactivating)

   Promoter

   Available Since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDH-EF1a-tetR-T2A-iRFP

   Plasmid

   #176028

   Purpose

   construct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome iRFP720

   Depositor

   Irmela Jeremias

   Article

   Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

   Inserts

   tetR
   iRFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJK503

   Plasmid

   #155380

   Purpose

   dCas12a oscillator, same direction crRNAs. dCas12a (D917A) + PA4-mVenus

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas12a (F. novicida)
   PA4-mVenus
   dCas12a oscillator crRNAs
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D917A (nuclease-deactivating)

   Promoter

   Available Since

   May 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCEP4L-Erk1

   Plasmid

   #39225

   Depositor

   Melanie Cobb

   Article

   Frost et al Proc Natl Acad Sci U S A. 1994 Apr 26;91

   Insert

   Erk1 (MAPK3 Human)
   

   Use

   Episomal

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pCDH-EF1a-tetR-T2A-tSapphire

   Plasmid

   #176312

   Purpose

   construct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome t-Sapphire

   Depositor

   Irmela Jeremias

   Article

   Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

   Inserts

   tetR
   T-sapphire
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. RP78 pUAST Orco mRNA

   Plasmid

   #53431

   Purpose

   Expresses the full-length Drosophila melanogaster Orco cDNA under Gal4/UAS control

   Depositor

   Leslie Vosshall

   Article

   Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

   Insert

   Orco (Orco )
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Aug. 12, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pJK526

    Plasmid

    #155387

    Purpose

    PphlF-TA4 crRNAs, 1x cascade. dCas12a (D917A) + PA4-mVenus

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas12a (F. novicida)
    PA4-mVenus
    PphlF-TA4 in dCas12a oscillator crRNAs
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D917A (nuclease-deactivating)

    Promoter

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJK528

    Plasmid

    #155388

    Purpose

    dCas12a oscillator, reverse crRNAs + PA4-mVenus. (Integration of 511; pOSIP)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D917A (nuclease-deactivating)

    Promoter

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJK434

    Plasmid

    #155378

    Purpose

    PphlF_A4NT, 1x cascade + [dCas9 + PA4-mVenus]. (Integration of 429; pOSIP)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    PphlF_A4NT in dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available Since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJK422

    Plasmid

    #155373

    Purpose

    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 412; pOSIP)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available Since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJK423

    Plasmid

    #155374

    Purpose

    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 413; pOSIP)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available Since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAcUW51-CgE

    Plasmid

    #13762

    Depositor

    Pamela Bjorkman

    Article

    Sprague et al PLoS Biol. 2006 Jun . 4(6):e148.

    Insert

    HSV-1 glycoprotein E
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    C-terminal region of the HSV-1 gE (KOS strain ect…

    Promoter

    Available Since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pJK520

    Plasmid

    #155386

    Purpose

    PphlF-TA1 crRNAs, 2x cascade. dCas12a (D917A) + PA4-mVenus

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas12a (F. novicida)
    PA4-mVenus
    PphlF-TA1 in dCas12a oscillator crRNAs
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D917A (nuclease-deactivating)

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

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17. CAHS2 (pBS0249)

    Plasmid

    #185139

    Purpose

    For the mammalian expression of the tardigrade protein CAHS2. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    CAHS2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pB[vasa-Cas9 PUb-ZsGreen]

    Plasmid

    #169012

    Purpose

    For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

    Depositor

    Max Scott

    Article

    Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

    Insert

    Cas9
    

    Use

    Piggybac

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Drosophila melanogaster vasa

    Available Since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pB[nos-Cas9 PUb-ZsGreen]

    Plasmid

    #169011

    Purpose

    For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

    Depositor

    Max Scott

    Article

    Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

    Insert

    Cas9
    

    Use

    Piggybac

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Drosophila melanogaster nanos

    Available Since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MTNR1A-DuET

    Plasmid

    #213348

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    MTNR1A (MTNR1A Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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