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We narrowed to 63,680 results for: cloning

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Showing: 4581 - 4600 of 63680 results

  3. pCMV-T7-ABE8.20m-nSpRY-P2A-EGFP (KAC1335)

    Plasmid
    #185917
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ABE8.20m-nSpRY-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185917-plasmid-map-sequence-id-367357

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  4. QUIN

    Plasmid
    #184267
    Purpose
    A binary plasmid derived from pCambia1300, with an insert that is a full-length cDNA of CPSMV RNA1 flanked by CaMV 35S promoter and terminator.
    Depositor
    Feng Qu
    Publication
    Zaulda et al Plant Methods. 2022 Oct 28;18(1):116. do
    Insert
    CPSMV RNA1 cDNA with 4 introns
    Expression
    Plant
    Mutation
    S1098P, K1651R
    Promoter
    CaMV 35S with duplicated enhancer
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    184267-plasmid-map-sequence-id-363074

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  9. GCEPIA1-SNAPER

    Plasmid
    #172659
    Purpose
    Detect the real-time ER calcium store and local calcium microdomains in live cells.
    Depositor
    Pingyong Xu
    Publication
    Luo et al Biophys Rep (2019) 5, 31–42
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    calmodulin
    Expression
    Mammalian
    Mutation
    Please see depositor comments
    Available since
    Aug. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    172659-plasmid-map-sequence-id-339177

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  17. pcDNA3.1-GGamma1-GFP2

    Plasmid
    #140989
    Purpose
    Encodes a G gamma subunit (GNGT1) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GGamma1-GFP2 (GNGT1 Human)
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    140989-plasmid-map-sequence-id-302012

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  18. pDONR207 SARS-CoV-2 NSP3_nostop

    Plasmid
    #149306
    Purpose
    Gateway-compatible Entry vector, with insert of NSP3 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
    Depositor
    Fritz Roth
    Publication
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Insert
    NSP3 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway Cloning
    Mutation
    Many synonymous changes due to codon optimization
    Available since
    May 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    149306-plasmid-map-sequence-id-296433

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Showing: 4581 - 4600 of 63680 results