We narrowed to 8,688 results for: APP

1. pETM33_CRK_SH3

   Plasmid

   #178472

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   CRK_SH3 (CRK Human, Synthetic)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7/LacO

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO.1 - TRC cloning vector

   Plasmid

   #10878

   Purpose

   3rd generation transfer plasmid

   Depositor

   David Root

   Article

   Moffat et al Cell. 2006 Mar 24. 124(6):1283-98.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. CROPseq-iT7

   Plasmid

   #211699

   Purpose

   NIS-Seq-compatible lentiviral vector for expressing sgRNAs

   Depositor

   Jonathan Schmid-Burgk

   Article

   Fandrey et al Nat Biotechnol. 2024 Dec 19. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmTurquoise2-LAMP1

   Plasmid

   #110948

   Purpose

   Expresses LAMP1 fused to mTurquoise2

   Depositor

   Pablo Rivera-Fuentes

   Article

   Halabi et al Nat Commun. 2019 Mar 15;10(1):1232. doi:

   Insert

   Lysosomal-associated membrane protein 1 (LAMP1 Human)
   

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA4-NHK-MYC-His6

   Plasmid

   #185350

   Purpose

   Assessing of ubiquitination in cells

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   a1-antitrypsin Hong Kong Null mutant
   

   Use

   Tags

   Myc, 6xHis

   Expression

   Mammalian

   Mutation

   NHK mutant

   Promoter

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO.1 - TRC control

   Plasmid

   #10879

   Purpose

   Negative control shRNA vector containing non-hairpin insert.

   Depositor

   David Root

   Article

   Moffat et al Cell. 2006 Mar 24. 124(6):1283-98.

   Insert

   non-hairpin 18bp
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFR WT

   Plasmid

   #11011

   Purpose

   Retroviral construct for expressing human EGFR in human cells

   Depositor

   Matthew Meyerson

   Article

   Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

   Insert

   EGFR (EGFR Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-pA-Hia5

   Plasmid

   #174372

   Purpose

   SEE DEPOSITOR COMMENTS BELOW. Plasmid for IPTG-inducible expression and purification of protein A-Hia5-6xHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

   Depositor

   Aaron Straight

   Article

   Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

   Insert

   protein A - hia5
   

   Use

   Tags

   6xHis and Protein A

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. MIB2-FLAG

   Plasmid

   #37117

   Depositor

   Martin Dorf

   Article

   Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

   Insert

   MIB2, mindbomb E3 ubiquitin protein ligase 2 (MIB2 Human)
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pET-pAG-Hia5

    Plasmid

    #174373

    Purpose

    Plasmid for IPTG-inducible expression and purification of protein A/G-Hia5-6xHis (pAG-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

    Depositor

    Aaron Straight

    Article

    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

    Insert

    protein AG - hia5
    

    Use

    Tags

    6xHis and Protein A/G

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEasyG2_mic

    Plasmid

    #184916

    Purpose

    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_mic recombines in vivo with a PCR product from pEasyG2_zeo/nat/hph.

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMC234r-ccdB/TPK2

    Plasmid

    #176179

    Purpose

    yeast MoClo level-0 part plasmid type 234r containing toxic gene dropout cassette (ccdB and TPK2)

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    dropout cassette containing toxic genes ccdB and TPK2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Cas12aUltra_5XNLS

    Plasmid

    #218775

    Purpose

    His-TEV-Cas12aUltra-BPSV40_cMyc_NP_5NLS

    Depositor

    Ross Wilson

    Article

    Sahu et al bioRxiv 2024.07.14.603391

    Insert

    5xNLS-BPSV40-NLP-cMyc-cMyc-BPSV40- AspCas12a
    

    Use

    CRISPR

    Tags

    6XHis (C terminal), HA, Nucleoplasmin, SV40, and …

    Expression

    Bacterial

    Mutation

    Nucleotides mutations were made to encode for arg…

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. DHB-mVenus

    Plasmid

    #136461

    Purpose

    Fluorescently tagged segment of DNA Helicase B that translocates from the nucleus to the cytoplasm in response to phosphorylation by CDK2

    Depositor

    Sabrina Spencer, Tobias Meyer

    Article

    Spencer et al Cell. 2013 Oct 10;155(2):369-83. doi: 10

    Insert

    DNA Helicase B (HELB Human)
    

    Use

    Lentiviral

    Tags

    mVenus

    Expression

    Mutation

    Promoter

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-HA-UTX

    Plasmid

    #24168

    Purpose

    Mammalian expression of human UTX with HA tag

    Depositor

    Kristian Helin

    Article

    Agger et al Nature. 2007 Oct 11. 449(7163):731-4.

    Insert

    UTX (KDM6A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG PSAM4 5HT3 LC IRES GFP

    Plasmid

    #123066

    Purpose

    Chemogenetic activator expression plasmid

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Insert

    PSAM4 5HT3 LC IRES GFP (Htr3a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L131G Q139L Y217F

    Promoter

    CAG

    Available since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG PSAM4 GlyR IRES EGFP

    Plasmid

    #119739

    Purpose

    Chemogenetic inhibitor expression plasmid

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Insert

    PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L131G, Q139L, Y217F

    Promoter

    CAG

    Available since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. MSCV-Luciferase-IRES-NrasG12D

    Plasmid

    #60834

    Purpose

    Mouse NrasG12D was amplified and mutated by PCR from IMAGE clone 6475312 (Invitrogen) and cloned downstream from the IRES; subsequently Luciferase was cloned upstream of the IRES

    Depositor

    Scott Lowe

    Article

    Zuber et al Genes Dev. 2009 Apr 1;23(7):877-89. doi:

    Insert

    NrasG12D (Nras Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOPINB-OTULIN (full-length, aa 1-352)

    Plasmid

    #61464

    Purpose

    Expresses human OTULIN (full-length) in E. coli.

    Depositor

    David Komander

    Article

    Keusekotten et al Cell. 2013 Jun 6;153(6):1312-26. doi: 10

    Insert

    OTULIN (OTULIN Human)
    

    Use

    Tags

    His6-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. EGFP-Ubiquitin

    Plasmid

    #187910

    Purpose

    Mammalian cell expression of EGFP-tagged Ubiquitin

    Depositor

    Erika Holzbaur

    Article

    Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

    Insert

    UBB (UBB Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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