We narrowed to 4,691 results for: POR C

1. EGFP-hPCM2w/b delta6

   Plasmid

   #47588

   Depositor

   Emanuel Strehler

   Article

   Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

   Insert

   PMCA2w/b delta6 (ATP2B2 Human)
   

   Use

   Tags

   EGFP-HA-His

   Expression

   Mammalian

   Mutation

   6 C-terminal AA deleted (needed for PDZ binding)

   Promoter

   CMV

   Available Since

   Oct. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSV CDK9 (D167N) HA (P#601)

   Plasmid

   #14639

   Depositor

   Matija Peterlin

   Article

   Fujinaga et al J Virol. 1998 Sep . 72(9):7154-9.

   Insert

   cdk9 D167N (CDK9 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D167N. Kinase negative mutant.

   Promoter

   Available Since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. EGFP-hPMCA2x/b delta6

   Plasmid

   #47587

   Depositor

   Emanuel Strehler

   Article

   Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

   Insert

   PMCA2x/b delta6 (ATP2B2 Human)
   

   Use

   Tags

   EGFP-HA-His

   Expression

   Mammalian

   Mutation

   6 C-terminal AA deleted (needed for PDZ binding)

   Promoter

   CMV

   Available Since

   Oct. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pMCSG49-AlleyCat7

   Plasmid

   #42591

   Depositor

   Ivan Korendovych

   Article

   Moroz et al Angew Chem Int Ed Engl. 2013 Apr 29. doi

   Insert

   mutated C-terminal portion of calmodulin gene (M76-K148)
   

   Use

   Tags

   His and TEV

   Expression

   Bacterial

   Mutation

   changed Phenylalanine 92 to Glutamine acid, Isole…

   Promoter

   T7

   Available Since

   March 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. Rev CDK9 (D167N) (P#858)

   Plasmid

   #14635

   Depositor

   Matija Peterlin

   Article

   Fujinaga et al J Virol. 1998 Sep . 72(9):7154-9.

   Insert

   cdk9 D167N (CDK9 Human)
   

   Use

   Tags

   Rev

   Expression

   Mammalian

   Mutation

   D167N. Kinase negative mutant.

   Promoter

   Available Since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pEGFP-PRKCZ-C1 (N-PRKCZ-C1)

   Plasmid

   #217760

   Purpose

   Fluorescent reporter for ceramide (putative, mammalian expression)

   Depositor

   Paula Nunes-Hasler

   Article

   Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

   Insert

   Protein kinase C zeta (PRKCZ Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C1 domain (aa 123-193)

   Promoter

   CMV

   Available Since

   June 11, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pHW582

   Plasmid

   #198819

   Purpose

   In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, higher baseline fluorescence

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   15xUAS::GCaMP7b-SL2-mKate2::let-858 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJR012

   Plasmid

   #198823

   Purpose

   Nucleolus-transport-enhanced Cre protein. Recombination-mediated gene expression knockout.

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   15xUAS::nCRE-SL2-GFP::let-858 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHW580

   Plasmid

   #198816

   Purpose

   In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, slow kinetics

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   15xUAS::GCaMP7s-SL2-mKate2::let-858 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 5ZiPro

    Plasmid

    #198432

    Purpose

    Conformation sensitive site specific Cys-labeling

    Depositor

    Tanja Schirmeister, Ute Hellmich

    Article

    Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

    Insert

    glycine linked Zika virus NS2B-NS3
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    S84*C, R95*A, C80S, C143S, S160C, C178S,

    Promoter

    T7 promoter

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1(+)-RasAR-NES

    Plasmid

    #205567

    Purpose

    Genetically encoded FRET-based biosensor for monitoring cellular Ras GTPase activity. Targeted to cytosol.

    Depositor

    Jin Zhang

    Article

    Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

    Insert

    RasAR-NES (RAF1 Synthetic, Human)
    

    Use

    Tags

    Nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAR17_StrepI_hFASh_H8_pET22b

    Plasmid

    #122846

    Purpose

    Expresses human type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.

    Depositor

    Martin Grininger

    Article

    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

    Insert

    human FASN/ human fatty acid synthase (FASN Human)
    

    Use

    Tags

    His8 tag and Twin-Strep tag

    Expression

    Bacterial

    Mutation

    wildtype

    Promoter

    T7 promoter

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR223_HyPer7RgDAAO

    Plasmid

    #217746

    Purpose

    Donor vector for gateway cloning of HyPer7 DAAO, a fusion protein used to measure the uptake of D-amino acids across the plasma membrane.

    Depositor

    Seth Parker

    Article

    Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi

    Insert

    HyPer7 D amino acid oxidase
    

    Use

    Tags

    Nuclear export signal

    Expression

    Mammalian

    Mutation

    Fused DAAO to the C-terminus of HyPer7 using a Gl…

    Promoter

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pS6-hα2M-H6

    Plasmid

    #128495

    Purpose

    Expresses human α2M (from G27 to S1468) in mammalian cells. With C-t H6x tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    A2M (G27-S1468) (A2M Human)
    

    Use

    Tags

    His tag (6x)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAR69_STRI_m(KS_MAT)_H8_pET22b

    Plasmid

    #122849

    Purpose

    Expresses the condensing part of murine type I fatty acid synthase (FASN) consisting of the KS and MAT domain in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.

    Depositor

    Martin Grininger

    Article

    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

    Insert

    m(KS_MAT) (Fasn Mouse)
    

    Use

    Tags

    His8 tag and Twin-Strep tag

    Expression

    Bacterial

    Mutation

    wildtype

    Promoter

    T7 promoter

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEGFP-PRKCZ-C20 (N-PRKCZ-C20)

    Plasmid

    #217762

    Purpose

    Fluorescent reporter for ceramide (putative, mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Protein kinase C zeta (PRKCZ Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Linker GGSSGGGA plus C20 domain (aa 405-592)

    Promoter

    CMV

    Available Since

    June 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pS6-N-hα2M-H6

    Plasmid

    #128496

    Purpose

    Expresses human N-α2M (from G27 to A788) in mammalian cells. With C-t H6x tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    A2M (G27-A788) (A2M Human)
    

    Use

    Tags

    His tag (6x)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PB-TAC-OK+9MS

    Plasmid

    #80482

    Purpose

    piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry)

    Depositor

    Knut Woltjen

    Article

    Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

    Insert

    OK+9MS (Pou5f1, Klf4, Myc, Sox2 Mouse)
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Klf4 [1-483]

    Promoter

    tetO

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1(+)-RasAR(R89L)-NES

    Plasmid

    #205568

    Purpose

    RasAR negative-control mutant plasmid. Targeted to cytosol.

    Depositor

    Jin Zhang

    Article

    Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

    Insert

    RasAR(R89L)-NES (RAF1 Synthetic, Human)
    

    Use

    Tags

    Nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    Arg 89 mutated to Leu in Raf1 RBD region.

    Promoter

    CMV

    Available Since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MCS-3_Endofin_LgBiT

    Plasmid

    #182574

    Purpose

    This plasmid can be used to monitor receptor internalization in real time living cells using bioluminescence as a readout.

    Depositor

    Bradley McConnell

    Article

    Reyes-Alcaraz et al Commun Biol. 2022 Mar 8;5(1):212. doi: 1

    Insert

    zinc finger FYVE-type (ZFYVE16 Human)
    

    Use

    Tags

    LgBiT (Large BiT)

    Expression

    Mammalian

    Mutation

    FYVE domain Q739-K806

    Promoter

    Herpes simplex virus (HSV)

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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