We narrowed to 5,906 results for: pcDNA 3.1

1. GFP-V96 ELP

   Plasmid

   #67853

   Purpose

   GFP Fluorescent Elastin-like Polypeptide(VPGVG) with 96 repeats containing Valine guest residues

   Depositor

   Andrew MacKay

   Article

   Pastuszka et al Biomacromolecules. 2012 Nov 12;13(11):34

   Insert

   GFP fused to ELP (VPGVG) repeated 96 times
   

   Use

   Tags

   GFP fused to N-terminal of ELP

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. untargeted Plk1 FRET sensor Myt1 substrate

   Plasmid

   #45217

   Depositor

   Michael Lampson

   Article

   Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

   Insert

   untargeted Plk1 FRET sensor Myt1 substrate
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   t7

   Available since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. CENP-B targeted Aurora B FRET sensor Kif2 substrate

   Plasmid

   #45214

   Depositor

   Michael Lampson

   Article

   Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

   Insert

   CENP-B targeted Aurora B FRET sensor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   t7

   Available since

   May 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV2-hNCOA6:V5

   Plasmid

   #63585

   Purpose

   A subunit of the Trithorax-related (Trr) methyltransferase complex.

   Depositor

   Kenneth Irvine

   Article

   Oh et al Cell Rep. 2014 Jul 24;8(2):449-59. doi:

   Insert

   NCOA6 (NCOA6 Human)
   

   Use

   Tags

   6xHis and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pmiRFP670nano3-NbGFP

   Plasmid

   #184686

   Purpose

   Nanobody to GFP labelled with near-infrared fluorescent protein miRFP670nano3

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   miRFP670nano3-NbGFP
   

   Use

   Tags

   miRFP670nano3

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pspCas9-3'UTR-Tetra-com-vector

   Plasmid

   #132553

   Purpose

   Expresses spCas9 and sgRNA scaffold with com aptamer replacing the Tetraloop

   Depositor

   Baisong Lu

   Article

   Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

   Inserts

   Cas9
   sgRNA scaffold with com replacing the Tetraloop
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. mSIRT3-S

   Plasmid

   #33310

   Depositor

   Michael Sack

   Article

   Bao et al J Cell Biochem. 2010 May;110(1):238-47.

   Insert

   Sirt3 (Sirt3 Mouse)
   

   Use

   Tags

   3XFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 2, 2012

   Availability

   Academic Institutions and Nonprofits only

8. optGluCl alpha EYFP

   Plasmid

   #15104

   Depositor

   Henry Lester

   Article

   Slimko et al J Neurosci Methods. 2003 Mar 30. 124(1):

   Insert

   GluCl alpha (glc-1 Nematode)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Codon optimized cDNA

   Promoter

   Available since

   June 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. HA-EcPPK1

   Plasmid

   #159279

   Purpose

   Expresses a HA-tagged E. coli PPK1 in mammalian cell lines

   Depositor

   Michael Downey

   Article

   Bondy-Chorney et al Cell Rep. 2020 Oct 27;33(4):108318. doi:

   Insert

   HA-tagged Polyphosphate Kinase 1
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. CMV-2xFLAG-Atoh1

    Plasmid

    #101734

    Purpose

    Expresses mouse Atoh1 with FLAG tag

    Depositor

    Albert Edge

    Article

    Parker et al Hum Gene Ther Methods. 2014 Feb;25(1):1-

    Insert

    Atoh1 (Atoh1 Mouse)
    

    Use

    Tags

    2x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNIR-FbGFPY58/C-JIP

    Plasmid

    #184684

    Purpose

    NIR-Fb-based JNK inhibitor

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    NIR-FbGFPY58/C-JIP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. WNK2 K207A

    Plasmid

    #24570

    Depositor

    Joseph Costello

    Article

    Hong et al Proc Natl Acad Sci U S A. 2007 Jun 26. 1

    Insert

    WNK2` (WNK2 Human)
    

    Use

    Tags

    His6 and V5

    Expression

    Mammalian

    Mutation

    K207A

    Promoter

    Available since

    June 11, 2010

    Availability

    Academic Institutions and Nonprofits only

13. tCRMod-GGSGGS7

    Plasmid

    #111761

    Purpose

    Expresses soluble Clover-mRuby2 tension sensor module with 7-repeat GGSGGS extensible domain

    Depositor

    Brenton Hoffman

    Article

    LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

    Insert

    Clover-GGSGGS7-mRuby2 fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 1-228 of Clover, residues 1-3 deleted fr…

    Promoter

    CMV

    Available since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGAL4-DBD-MCS-long

    Plasmid

    #145246

    Purpose

    Backbone vector expressing GAL4 DNA-binding domain followed by MCS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. FLAG-ZnF/HIT1

    Plasmid

    #15332

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Cai et al J Biol Chem. 2005 Apr 8. 280(14):13665-7

    Insert

    zinc finger, HIT domain containing 1 (ZNHIT1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

16. tCRMod-GGSGGS5

    Plasmid

    #111760

    Purpose

    Expresses soluble Clover-mRuby2 tension sensor module with 5-repeat GGSGGS extensible domain

    Depositor

    Brenton Hoffman

    Article

    LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

    Insert

    Clover-GGSGGS5-mRuby2 fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 1-228 of Clover, residues 1-3 deleted fr…

    Promoter

    CMV

    Available since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Lmajor_gp63_10_0460_E265A

    Plasmid

    #171641

    Purpose

    Expression of catalytically inactive L. major glcyoprotein-63 (E265A) from chromosome 10 in mammalian cells

    Depositor

    Dennis Ko

    Article

    Antonia et al Front Cell Infect Microbiol. 2019 Aug 7;

    Insert

    LmjF.10.0460 E265A
    

    Use

    Tags

    Myc-His

    Expression

    Mammalian

    Mutation

    E265A

    Promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. [Bla][IRES][GFP]

    Plasmid

    #97193

    Purpose

    Vector for our translation quantification assay (Fig. 5). Double reporter vector with internal ribosome entry site.

    Depositor

    Edward Boyden

    Article

    Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Inserts

    beta-Lactamase
    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMM585-Tier1-PhCMV-Igk

    Plasmid

    #169486

    Purpose

    Tier-1 vector encoding PhCMV-driven secretion signal Igk (PhCMV-Igk-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven Igk secretion signal (IGK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pB2AR-miRFP670nano3

    Plasmid

    #184671

    Purpose

    Beta-2-adrenergic receptor internally tagged with miRFP670nano3

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    B2AR-miRFP670nano3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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