We narrowed to 11,735 results for: pcdna

1. pcDNA3-mCherry-TSC2-NLS

   Plasmid

   #192441

   Purpose

   Encodes mCherry-tagged wild-type TSC2 isoform 5 targeted to the nucleus.

   Depositor

   Jin Zhang

   Article

   Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

   Insert

   mCherry-TSC2-NLS (TSC2 Human)
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-mCherry-TSC2(AA)-NLS

   Plasmid

   #192443

   Purpose

   Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation; targeted to the nucleus.

   Depositor

   Jin Zhang

   Article

   Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

   Insert

   mCherry-TSC2(S939A,T1439A)-NLS (TSC2 Human)
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

   Promoter

   CMV

   Available since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.2/V5 mmu-mir-122

   Plasmid

   #26325

   Depositor

   David Bartel

   Article

   Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

   Insert

   mmu-mir-122
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3 Connexin43-GFP-APEX

   Plasmid

   #44440

   Depositor

   Alice Ting

   Article

   Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103

   Insert

   Connexin43 -EGFP - pea APEX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K14D, W41F, E112K mutations on APEX

   Promoter

   CMV

   Available since

   May 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-HA-REDD1 48-232 (#182)

   Plasmid

   #99920

   Purpose

   mammalian expression

   Depositor

   James Brugarolas

   Article

   Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

   Insert

   REDD1 (DDIT4 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   N-terminal deletion; encodes AA 48-232

   Promoter

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA6-LRRC55-V5-Flag-His

   Plasmid

   #127819

   Purpose

   Expression of human LRRC55 in mammalian cells

   Depositor

   Jiusheng Yan

   Article

   Li et al J Gen Physiol. 2015 Jun;145(6):543-54. d

   Insert

   LRRC55 (LRRC55 Human)
   

   Use

   Tags

   V5, FLAG, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-HCMVgH-8his (TB40/E)

   Plasmid

   #136445

   Purpose

   Mammalian expression plasmid for HCMV glycoprotein H (TB40/E strain) extracellular domain with 8his purification tag

   Depositor

   Erik Procko

   Article

   Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

   Insert

   glycoprotein H (UL75 Human betaherpesvirus 5)
   

   Use

   Tags

   Short GGSG linker and 8his tag

   Expression

   Mammalian

   Mutation

   Extracellular domain (a.a. 1-717). Codon-optimize…

   Promoter

   CMV

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA5/FRT_CMV_NES-Caprola_04-mEGFP

   Plasmid

   #194682

   Purpose

   CMV driven expression of the calcium recorder Caprola_04 in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_04-mEGFP
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3.1-Flag-H2A K13R

   Plasmid

   #63566

   Purpose

   Expression of human H2A with K13R tagged with Flag

   Depositor

   Titia Sixma

   Article

   Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

   Insert

   H2A (H2AC20 Human)
   

   Use

   Tags

   Flag (MDYKDDDDKVPRIQ)

   Expression

   Mammalian

   Mutation

   Lysine at 13 is now Arginine

   Promoter

   CMV

   Available since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pCDNA3.1-Flag-H2A K15R

    Plasmid

    #63567

    Purpose

    Expression of human H2A with K15R tagged with Flag

    Depositor

    Titia Sixma

    Article

    Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

    Insert

    H2A (H2AC20 Human)
    

    Use

    Tags

    Flag (MDYKDDDDKVPRIQ)

    Expression

    Mammalian

    Mutation

    Lysine at 15 is now Arginine

    Promoter

    CMV

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3-ELMOD1-myc-His6

    Plasmid

    #201900

    Purpose

    Used for expression of human protein ELMOD1 in mammalian cells

    Depositor

    Richard Kahn

    Insert

    ELMOD1 (ELMOD1 Human)
    

    Use

    Tags

    myc, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-TRBP-L1+D1+L2-myc/His

    Plasmid

    #180599

    Purpose

    Expressing various parts of TRBP

    Depositor

    Kumiko Ui-Tei

    Article

    Takahashi et al Nucleic Acids Res. 2018 Sep 28;46(17):91

    Insert

    TRBP (TARBP2 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5-eYFP-FKBP-MAD1-AA

    Plasmid

    #114038

    Purpose

    Chemical induction of MAD1 localisation to a specific site in the cell (by rapamycin & FRB)

    Depositor

    Geert Kops

    Article

    Kuijt et al Chromosoma. 2014 Oct;123(5):471-80. doi:

    Insert

    Human MAD1 (MAD1L1 Human)
    

    Use

    Tags

    eYFP-FKBP12

    Expression

    Mammalian

    Mutation

    MAD2 binding deficient MAD1 K541A/L543A

    Promoter

    CMV

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-MAVS-M1A(KaganF58)

    Plasmid

    #52001

    Purpose

    Expresses the human MAVS CDS containing the point mutation M1A

    Depositor

    Jonathan Kagan

    Article

    Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

    Insert

    MAVS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Met 1 to Ala

    Promoter

    Available since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1-hRIPK1-Flag-S320A

    Plasmid

    #112491

    Purpose

    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.

    Depositor

    Junying Yuan

    Insert

    RIPK1 (RIPK1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S320A

    Promoter

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3 PBD wt (Nigg AH12)

    Plasmid

    #41162

    Depositor

    Erich Nigg

    Article

    Hanisch et al Mol Biol Cell. 2006 Jan;17(1):448-59. Ep

    Insert

    PBD (PLK1 Human)
    

    Use

    Tags

    3xMyc and PreScission Site

    Expression

    Mammalian

    Mutation

    Contains Plk1-PBD only (aa 326–603)

    Promoter

    CMV

    Available since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA-NBid-PhoCl1-CBid

    Plasmid

    #164050

    Purpose

    Optogenetic manipulation of cell apoptosis using PhoCl1

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    NBid-PhoCl1-CBid
    

    Use

    Tags

    kozak sequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promotor

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-Flt3-Y591F/Y919F

    Plasmid

    #74497

    Purpose

    Overexpression of FLT3 Y591F/Y919F mutant in mammalian cells

    Depositor

    Lars Rönnstrand

    Article

    Kazi et al J Biol Chem. 2012 Oct 19;287(43):36509-1

    Insert

    FLT3 (FLT3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Y591F/Y919F, T227M

    Promoter

    CMV

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA 3.2-FUS-1-373aa-V5

    Plasmid

    #29611

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS-1-373aa (FUS Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Contains FUS aa# 1-373

    Promoter

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

20. WFS1 P724L-Flag-pcDNA3

    Plasmid

    #13012

    Depositor

    Fumihiko Urano

    Article

    Fonseca et al J Biol Chem. 2005 Nov 25. 280(47):39609-

    Insert

    Wolfram Syndrome 1 (WFS1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    changed P 724 to L

    Promoter

    Available since

    Sept. 26, 2006

    Availability

    Academic Institutions and Nonprofits only