165,214 results
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Plasmid#227139PurposeBarcoded assay, Ca2+ sensor; barcode BC0524DepositorInsert6x clustered NFAT element linked to CMV minimal promoter driving barcode 0524 and a luciferase reporter gene
ExpressionMammalianAvailable SinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX552-hsyn DIO GCaMP8f(with gRNA scaffold)
Plasmid#199580PurposeExpresses GCaMP8f in a Cre-dependent mannerDepositorInsertHis-DIO GCaMP8f
UseAAV, CRISPR, and Cre/LoxTags6xHisExpressionMammalianPromoterhsynAvailable SinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pscALPSpuro-MDA5-S88D
Plasmid#174226PurposeExpresses human MDA5 S88D mutantDepositorAvailable SinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV-IgK-pHluorin-TM-mRuby
Plasmid#185546PurposeExpresses surface-localized pHluorin fused to intracellular expression of mRuby under CMV promoterDepositorInsertIgK-pHluorin-TM-mRuby
ExpressionMammalianPromoterCMVAvailable SinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
MTNR1B-DuET
Plasmid#213349PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMKLR1-DuET
Plasmid#213212PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL_CDC20:161-477
Plasmid#210943PurposeInsect expression of human CDC20 fragment S161 to P477. N-terminal 6X His tag with TEV cleavage.DepositorInsertCDC20:S161-P477 (CDC20 Human)
TagsMHHHHHHSSGRENLYFQGExpressionInsectMutationwild typePromoterPolyhedrinAvailable SinceMarch 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNLF1-C_CDC20:1-499
Plasmid#211062PurposeMammalian expression of full-length human CDC20. C-terminal NanoLuc luciferase tag.DepositorInsertCDC20:M1-R499 (CDC20 Human)
TagsNanoLuc luciferaseExpressionMammalianMutationwild typePromoterCMVAvailable SinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-N_CDC20:1-499
Plasmid#211058PurposeMammalian expression of full-length human CDC20. N-terminal NanoLuc luciferase tag.DepositorInsertCDC20:M1-R499 (CDC20 Human)
TagsNanoLuc luciferaseExpressionMammalianMutationwild typePromoterCMVAvailable SinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_CDC20:1-499
Plasmid#211122PurposeMammalian expression of full-length human CDC20. N-terminal HiBiT tag.DepositorInsertCDC20:M1-R499 (CDC20 Human)
TagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationwild typePromoterCMVAvailable SinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only