We narrowed to 6,994 results for: ef1a

1. pJT331 Lenti pEF-dCas9-3XFLAG-Q+N+Z+F-T2A-tagBFP-P2A-Blast

   Plasmid

   #195538

   Purpose

   dCas9 effector fusion for manipulating transcription

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Lenti pEF-dCas9-3XFLAG-Q+N+Z+F-T2A-tagBFP-P2A-Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   pEF1a

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUC-hU6-shBACH1_BFP

   Plasmid

   #199217

   Purpose

   Expression plasmid with shRNAmiR targeting BACH1, with a BFP reporter on the backbone. 

   Depositor

   Gabor Balazsi

   Article

   Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

   Insert

   shBACH1 (BACH1 Synthetic)
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   human U6

   Available since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC_hU6-shRKIP_BFP

   Plasmid

   #199216

   Purpose

   Expression plasmid with shRNAmiR targeting RKIP (PEBP1), with a BFP reporter on the backbone.

   Depositor

   Gabor Balazsi

   Article

   Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

   Insert

   shBACH1 (BACH1 Synthetic)
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   human U6

   Available since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Donor_ANKRD1_KO_Hygro

   Plasmid

   #186668

   Purpose

   Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, hygromycin and polyA sequence is inserted between two homology arms.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   ANKRD1 KO Hygromycin (ANKRD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLEX307_PDHA2

   Plasmid

   #115192

   Purpose

   Lentiviral transduction and expression of PDHA2 into any mammalian cell

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   PDHA2WT (PDHA2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1alpha

   Available since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLEX307_PDHA2S291A

   Plasmid

   #115196

   Purpose

   Lentiviral transduction and expression of PDHA2S291A into any mammalian cell

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   PDHA2S291A (PDHA2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   p.S291A

   Promoter

   EF1alpha

   Available since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLEX307_PDHA2S291A/S293A

   Plasmid

   #115198

   Purpose

   Lentiviral transduction and expression of PDHA2S291A/S293A into any mammalian cell

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   PDHA2S291A/S293A (PDHA2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   p.S291A/S293A

   Promoter

   EF1alpha

   Available since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. HA tagged SphI ELL2 M133, 138, 186ILeu

   Plasmid

   #127277

   Purpose

   Expresses human ELL2 with M133, 138, 186ILeu to prevent internal Met initiation peptides and contains HA tag near C-terminus

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 with Met 133, 138, 186 to Ileu (ELL2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Met 133, 138, 186 to Ileu; HA inserted at SphI af…

   Promoter

   pEF1a

   Available since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLKO.1-shDnd1-No1

   Plasmid

   #70058

   Purpose

   TRC lentiviral vector for shRNA against mouse Dnd1 #1

   Depositor

   Thomas Tuschl

   Article

   Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

   Insert

   Dnd1 (Dnd1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Nov. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLKO.1-shDnd1-No3

    Plasmid

    #70060

    Purpose

    TRC lentiviral vector for shRNA against mouse Dnd1 #3

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Insert

    Dnd1 (Dnd1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLKO.1-shDnd1-No4

    Plasmid

    #70065

    Purpose

    TRC lentiviral vector for shRNA against mouse Dnd1 #4

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Insert

    Dnd1 (Dnd1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

12. pLKO.1-shDnd1-No5

    Plasmid

    #70066

    Purpose

    TRC lentiviral vector for shRNA against mouse Dnd1 #5

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Insert

    Dnd1 (Dnd1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLKO.1-shDnd1-No6

    Plasmid

    #70067

    Purpose

    TRC lentiviral vector for shRNA against mouse Dnd1 #6

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Insert

    Dnd1 (Dnd1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

14. pLKO.1-shDnd1-No8

    Plasmid

    #70069

    Purpose

    TRC lentiviral vector for shRNA against mouse Dnd1 #8

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Insert

    Dnd1 (Dnd1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

15. pLX313-TP53-WT

    Plasmid

    #118014

    Purpose

    Constitutive expression of wild-type human Tumor Protein p53 (TP53)

    Depositor

    David Root, William Hahn

    Article

    Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

    Insert

    TP53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJT300 Lenti pEF-dCas9-3XFLAG-ZNF705_KRAB-T2A-tagBFP-P2A-Blast

    Plasmid

    #195542

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Lenti pEF-dCas9-3XFLAG-ZNF705_KRAB-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EGFP-p300 (D1399Y)

    Plasmid

    #191763

    Purpose

    Expression of EGFP fused to catalytically dead core p300 histone acetyltransferase (D1399Y)

    Depositor

    Daniel Gerlich

    Article

    Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

    Insert

    FRB-EGFP-p300 core (D1399Y) (EP300 Human)
    

    Use

    Tags

    EGFP (N-terminal of p300 core (D1399Y)) and FRB (…

    Expression

    Bacterial and Mammalian

    Mutation

    catalytic D1399Y mutation in p300

    Promoter

    EF1alpha

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJT337 Lenti pEF-dCas9-3XFLAG-ZN471 KRAB-T2A-tagBFP-P2A-Blast

    Plasmid

    #187341

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-ZN471 KRAB-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJT055 AAVS1-BSD-9xTet0-pEF-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid

    #187324

    Purpose

    Donor to install a dual surface marker - fluorescence reporter into the AAVS1 safe harbor using HDR

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    BSD-9xTet0-pEF-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
    

    Use

    CRISPR and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLX313-TP53-P278A

    Plasmid

    #118015

    Purpose

    Constitutive expression of mutant human Tumor Protein p53 (TP53)

    Depositor

    David Root, William Hahn

    Article

    Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

    Insert

    TP53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    p.Pro278Ala

    Promoter

    EF1alpha

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJT151 lenti pEF-rTetR(SE-G72P)-3XFLAG-ZNF10 KRAB-T2A-mCherry-BSD-WPRE

    Plasmid

    #188757

    Purpose

    Lentiviral backbone containing ZNF10 KRAB as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    ZNF10 KRAB (ZNF10 Human)
    

    Use

    Lentiviral

    Tags

    rTetR(SE-G72P)-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMV9 lenti pEF-rTetR(SE-G72P)-3XFLAG-NOTC2(Notch)-T2A-mCherry-BSD-WPRE

    Plasmid

    #188755

    Purpose

    Lentiviral backbone containing NOTC2 Notch domain as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    NOTC2 Notch (NOTCH2 Human)
    

    Use

    Lentiviral

    Tags

    rTetR(SE-G72P)-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMV10 lenti pEF-rTetR(SE-G72P)-3XFLAG-WWP2(WW)-T2A-mCherry-BSD-WPRE

    Plasmid

    #188756

    Purpose

    Lentiviral backbone containing WWP2 WW domain as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    WWP2 WW (WWP2 Human)
    

    Use

    Lentiviral

    Tags

    rTetR(SE-G72P)-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PJT220 lenti pEF-rTetR(SE-G72P)-3XFLAG-ZNF473 KRAB-T2A-mCherry-BSD-WPRE

    Plasmid

    #188759

    Purpose

    Lentiviral backbone containing ZNF473 KRAB as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    ZNF473 KRAB (ZNF473 Human)
    

    Use

    Lentiviral

    Tags

    rTetR(SE-G72P)-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMV4 lenti pEF-rTetR(SE-G72P)-3XFLAG-ANM2(SH3_1)-T2A-mCherry-BSD-WPRE

    Plasmid

    #188750

    Purpose

    Lentiviral backbone containing ANM2 SH3_1 domain as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    ANM2 SH3_1 (PRMT2 Human)
    

    Use

    Lentiviral

    Tags

    rTetR(SE-G72P)-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMV8 lenti pEF-rTetR(SE-G72P)-3XFLAG-PYGO1(PHD)-T2A-mCherry-BSD-WPRE

    Plasmid

    #188754

    Purpose

    Lentiviral backbone containing PYGO1 PHD domain as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    PYGO1 PHD (PYGO1 Human)
    

    Use

    Lentiviral

    Tags

    rTetR(SE-G72P)-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJT340 Lenti pEF-dCas9-3XFLAG-KRAB[WSR7EEE] + MGA1+2 + RYBP-T2A-tagBFP-P2A-Blast

    Plasmid

    #187344

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-KRAB[WSR7EEE] + MGA1+2 + RYBP-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    KRAB WSR7EEE

    Promoter

    pEF1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJT241 Lenti pEF-dCas9-3XFLAG-WT ZNF10 KRAB-T2A-tagBFP-P2A-Blast

    Plasmid

    #187326

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-WT ZNF10 KRAB-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJT242 Lenti pEF-dCas9-3XFLAG-ZNF10 KRAB[WSR7EEE] HighExpr-T2A-tagBFP-P2A-Blast

    Plasmid

    #187327

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-ZNF10 KRAB[WSR7EEE] HighExpr-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    KRAB WSR7EEE

    Promoter

    pEF1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJT244 Lenti pEF-dCas9-3XFLAG-RYBP-T2A-tagBFP-P2A-Blast

    Plasmid

    #195537

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Lenti pEF-dCas9-3XFLAG-RYBP-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge