We narrowed to 5,358 results for: PID;

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pNK2657

Plasmid

#219741

Purpose

MoClo-compatible Level 0 promoterless vector encoding hispidin-synthase from Mycena citricolor codon-optimised for expression in Nicotiana benthamiana, Pichia pastoris, Homo sapiens

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

hispidin- synthase from Mycena citricolor

Use

Synthetic Biology

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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LCV2_mCherry

Plasmid

#155096

Purpose

Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and mCherry expression

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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LCV2_mClover3

Plasmid

#155095

Purpose

Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and mClover3 expression

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pBiFC-SS-VN155(I152L)-IRE1-dLD

Plasmid

#217752

Purpose

Expresses the VN fragment of BiFC-IRE1-dLD

Depositor

Christina Chan

Article

Cho et al Cell Rep. 2019 Apr 2;27(1):307-320.e5. d

Insert

Serine/threonine-protein kinase/endoribonuclease IRE1 (ERN1 Human)

Tags

Venus (1-154, I152L)

Expression

Mammalian

Mutation

deleted amino acids 29-408

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP-imp α

Plasmid

#119718

Purpose

Expresses EGFP-tagged human importinα in mammalian cells

Depositor

Kenji Sugimoto

Article

Nakagawa et al Biosci Biotechnol Biochem. 2012;76(2):38

Insert

importinα (KPNA2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

contains amino acids 251-529

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs-IRES-blast GFP-SPNS1

Plasmid

#198571

Purpose

SPNS1 codon-optimized orf with n-terminal 3xFlag GFP in pMXs-IRES-Blast vector

Depositor

Monther Abu-Remaileh

Article

Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

Insert

SPNS1 with n-terminal 3xFlag-GFP fusion (SPNS1 Human)

Use

Retroviral

Tags

3xFlag-GFP

Expression

Mammalian

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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Halo-SspB-PPM1H

Plasmid

#232870

Purpose

Expresses SspB-PPM1H in mammalian cells

Depositor

Pietro De Camilli

Article

Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

Insert

Halo-SspB-PPM1H (PPM1H )

Tags

Halo-SspB

Expression

Mammalian

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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AAVS1 intron 1 gRNA as2

Plasmid

#132394

Purpose

Targets AAVS1 intron 1, gRNA: AGAACCAGAGCCACATTAAC, MLM3636 backbone

Depositor

Gerold Schmitt-Ulms

Article

Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

Insert

gRNA targeting AAVS1 intron 1 (PPP1R12C Human)

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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mScarlet-LRRK1 K746G

Plasmid

#233586

Purpose

Expresses LRRK1 K746G in mammalian cells

Depositor

Pietro De Camilli

Article

Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

Insert

LRRK1 K746G (LRRK1 Human)

Tags

mScarlet

Expression

Mammalian

Available Since

June 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-NRG1-ScNeo

Plasmid

#209900

Purpose

To monitor the status of NRG1, the plasmid encodes a recombinant NRG1 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

NRG1-ScNeo (NRG1 Human)

Use

Lentiviral

Tags

mNeonGreen and mScarlet

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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LCV2_LacZ_sgRNA_2

Plasmid

#155093

Purpose

lentiviral plasmid expressing Cas9 and gRNA targeting LacZ

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

LacZ_sgRNA_2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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TFORF0211

Plasmid

#142484

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

FOXP3 (FOXP3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pPBpuro-NRG1-ScNeo

Plasmid

#209906

Purpose

To monitor the status of NRG1, the plasmid encodes a recombinant NRG1 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

NRG1-ScNeo (NRG1 Human)

Tags

mNeonGreen and mScarlet

Expression

Mammalian

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pBEL1200

Plasmid

#138526

Purpose

For expresson of mlaFEDCB operon from E. coli. N-terminal his tag on MlaD, araBAD promoter.

Depositor

Gira Bhabha, Damian Ekiert

Article

Ekiert et al Cell. 2017 Apr 6;169(2):273-285.e17. doi

Insert

mlaFEDCB (mlaF, mlaE, mlaD, mlaC, mlaB E. coli)

Tags

N-terminal His tag and TEV cleavage site on MlaD

Expression

Bacterial

Promoter

araBAD

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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H516 SONIC KrasG12D-IRES-luciferase donor

Plasmid

#138178

Purpose

CRISPR SONIC: Kras G12D luciferase donor plasmid.

Depositor

Wen Xue

Article

Mou et al Genome Med. 2019 Apr 16;11(1):21. doi: 1

Insert

KrasG12D (Kras Mouse)

Use

Nhej donor

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pAG426GAL-SLEV_NS2B-GS-NS3

Plasmid

#203510

Purpose

Expresses SLEV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

poly SLEV NS2B-GS-NS3 (POLY )

Expression

Yeast

Promoter

GAL1

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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PuroR-P2A-DD-TCOF1-donor

Plasmid

#60747

Purpose

Donor plasmid for HR-mediated recombination to insert puromycin resistance and append a destabilization domain to the N-terminus of TCOF1

Depositor

Benhur Lee

Article

Park et al PLoS One. 2014 Apr 17;9(4):e95101. doi:

Insert

TCOF1 (TCOF1 Human)

Use

Targeting donor

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
pJFRC7-JEDI-2P

Plasmid

#179461

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-2P under the promoter hsp70 for Drosophila (insect) expression

Depositor

Francois St-Pierre

Article

Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

Insert

JEDI-2P

Expression

Insect

Promoter

hsp70

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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BlastR-P2A-DD-TCOF1-donor

Plasmid

#60748

Purpose

Donor plasmid for HR-mediated recombination to insert blasticidin resistance and append a destabilization domain to the N-terminus of TCOF1

Depositor

Benhur Lee

Article

Park et al PLoS One. 2014 Apr 17;9(4):e95101. doi:

Insert

TCOF1 (TCOF1 Human)

Use

Targeting donor

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
LZRS-BRaf-ERTm

Plasmid

#24949

Depositor

Paul Khavari

Article

Dajee et al Oncogene. 2002 Feb 28;21(10):1527-38

Insert

BRaf-ER (Esr1, BRAF Mouse, Human)

Use

Retroviral

Tags

Myc

Mutation

V600E in BRAF

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only

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