We narrowed to 15,732 results for: grna

1. pT7-gRNA:Tyr (albino)

   Plasmid

   #60966

   Purpose

   Expression vector of rat Tyr (albino) guide RNA

   Depositor

   Tomoji Mashimo

   Article

   Yoshimi et al Nat Commun. 2014 Jun 26;5:4240. doi: 10.

   Insert

   Tyrosinase gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Jan. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pT7-gRNA:Tyr (wild)

   Plasmid

   #60967

   Purpose

   Expression vector of rat Tyr (wild) guide RNA

   Depositor

   Tomoji Mashimo

   Article

   Yoshimi et al Nat Commun. 2014 Jun 26;5:4240. doi: 10.

   Insert

   Tyrosinase gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pAAV-FLEX-SaCas9-U6-sgRNA

   Plasmid

   #124844

   Purpose

   Vector for Cre-dependent expression of SaCas9

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   SaCas9, gRNA scaffold
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   NLS and NLS-3xHA

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-FLEXfrt-SaCas9-U6-sgRNA

   Plasmid

   #124845

   Purpose

   Vector for FLP-dependent expression of SaCas9 with gRNA

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   SauCas9 gRNA scaffold
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP

   Plasmid

   #102796

   Purpose

   Retrovirus for delivery of one sgRNA (empty, bbsI)

   Depositor

   Sarah Teichmann

   Article

   Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

   Insert

   U6 sgRNA
   

   Use

   CRISPR and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKLV-U6gRNA(BbsI)-PGKpuro2ABFP

   Plasmid

   #50946

   Purpose

   An empty gRNA expression vector

   Depositor

   Kosuke Yusa

   Article

   Koike-Yusa et al Nat Biotechnol. 2014 Mar;32(3):267-73. d

   Insert

   None
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6-PspCas13b-gRNA-Actb1216

   Plasmid

   #155368

   Purpose

   PspCas13b guide RNA positioned 8bp 5' of Actb 1216 for targeted m6A RNA methylation

   Depositor

   David Liu

   Article

   Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

   Insert

   PspCas13b gRNA targeting Actb 1216
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAVS1 pegRNA1 with trimmed attP

   Plasmid

   #222346

   Purpose

   pegRNA 1 plasmid used for PASSIGE-mediated Bxb1 attP installation

   Depositor

   David Liu

   Article

   Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

   Insert

   AAVS1 attP-installation pegRNA1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAVS1 pegRNA2 with trimmed attP

   Plasmid

   #222347

   Purpose

   pegRNA 2 plasmid used for PASSIGE-mediated Bxb1 attP installation

   Depositor

   David Liu

   Article

   Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

   Insert

   AAVS1 attP-installation pegRNA2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti-Cas9-gRNA-TagBFP2

    Plasmid

    #124774

    Purpose

    3rd generation lentiviral plasmid encoding Cas9, TagBFP2, and a gRNA backbone

    Depositor

    Jason Sheltzer

    Article

    Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. mU6-sgRNA-CLTA-mCherry

    Plasmid

    #240637

    Purpose

    Vector for expressing an sgRNA targeting CLTA promoter from the mouse U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter.

    Depositor

    James Nuñez

    Article

    Xu et al Nat Commun. 2025 Aug 26;16(1):7948. doi:

    Insert

    puro-T2A-mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX459-HypaCas9-mR2-AAVS1_sgRNA

    Plasmid

    #183890

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and sgRNA targeting the AAVS1 in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    AAVS1 sgRNA spacer (AAVS1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. OpenCRISPR-1 sgRNA-16nt stem

    Plasmid

    #221566

    Purpose

    Express OpenCRISPR-1 sgRNA (16nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.

    Depositor

    Ali Madani

    Article

    Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586

    Insert

    OpenCRISPR-1 sgRNA 16nt stem loop
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 28, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pUC57-sgRNA expression vector

    Plasmid

    #51132

    Purpose

    For in vitro transcription of sgRNA from the T7 promoter.

    Depositor

    Xingxu Huang

    Article

    Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC57-Simple-gRNA backbone

    Plasmid

    #51306

    Purpose

    gRNA expression plasmid

    Depositor

    Yonglong Chen

    Article

    Guo et al Development. 2014 Jan 8.

    Type

    Empty backbone

    Use

    CRISPR; Xenopus expression

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. OpenCRISPR-1 sgRNA-12nt stem

    Plasmid

    #221567

    Purpose

    Express OpenCRISPR-1 sgRNA (12nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.

    Depositor

    Ali Madani

    Article

    Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586

    Insert

    OpenCRISPR-1 sgRNA 12nt stem loop
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 28, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. Lenti-Cas9-gRNA-GFP

    Plasmid

    #124770

    Purpose

    3rd generation lentiviral plasmid encoding Cas9, GFP, and a gRNA backbone

    Depositor

    Jason Sheltzer

    Article

    Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-U6-sgRNA-CMV-GFP

    Plasmid

    #85451

    Purpose

    Express sgRNA in mammalian cells

    Depositor

    Hetian Lei

    Article

    Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

    Insert

    pCMV-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    pCMV-EGFP

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSB700 lenti gRNA Blasto

    Plasmid

    #167904

    Purpose

    Lentiviral vector for expressing U6 sgRNA cloning plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. gRNA-CasRx_SD1-pSV40-TagBFP

    Plasmid

    #221004

    Purpose

    Transiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.

    Depositor

    Mikko Taipale, Benjamin Blencowe

    Article

    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

    Insert

    CasRx 30nt processed direct repeat with SD1 mutation
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    DR1 A>T

    Promoter

    U6

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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