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pETM33_SUFU_Sufu

Plasmid

#178488

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

SUFU_Sufu (SUFU Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pEJS469-pLK.O1-SpySgRNA/DTS13-Telomere

Plasmid

#85715

Purpose

Telomere-targeting Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Insert

telomere sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pET-21a_FnCpf1_2xNLS_protein_expression

Plasmid

#124548

Purpose

Protein expression plasmid for 2xNLS FnCpf1 in pET-21a backbone

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

FnCpf1_2xNLS

Use

CRISPR

Expression

Bacterial

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pJS646

Plasmid

#183175

Purpose

Y2H vector compatible with pJS647 PYR1 insert

Depositor

Timothy Whitehead

Article

Beltran et al Nat Biotechnol. 2022 Jun 20. pii: 10.103

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pFLAG-CMV2 ACAP1

Plasmid

#15697

Depositor

Paul Randazzo

Article

Jackson et al J Cell Biol. 2000 Oct 30. 151(3):627-38.

Insert

ACAP1 (ACAP1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Nov. 5, 2007

Availability

Academic Institutions and Nonprofits only
pAOJ15

Plasmid

#91567

Purpose

Broad host range allelic exchange suicide vector for bacteria - Tse2 toxin driven by anhydrotetracycline inducible promoter

Depositor

Harry Mobley

Article

Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.

Type

Empty backbone

Expression

Bacterial

Promoter

Tetracycline promoter

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pHD157

Plasmid

#84033

Purpose

Vector for creating transgenic zebrafish. Expresses mCherry-Cre fusion protein under the fabp10 promoter and Venus under the crystalin promoter in zebrafish

Depositor

Daniel Hesselson, Didier Stainier

Article

Ni et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Inserts

Cre
Venus

Use

Cre/Lox; Zebrafish transgenesis

Tags

mCherry

Promoter

Crystalin and fabp10

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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tol2-CAG::ChR2-YFP

Plasmid

#59740

Purpose

Optogenetic excitation of ES cell-derived neurons (and other cells, such as muscle)

Depositor

Ivo Lieberam

Article

Bryson et al Science. 2014 Apr 4;344(6179):94-7. doi:

Insert

Channelrhodopsin-2

Tags

YFP

Expression

Mammalian

Mutation

H134R

Promoter

CAG

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
OmpA-mNG-mCh

Plasmid

#124216

Purpose

Periplasmic OM bound mNeongreen mCherry tandem, ~16 % energy transfer. pNM004 - pTHV037-OmpA-SA1-177-(SA-1)-LEDPPAEL-mNG-mCh

Depositor

Tanneke den Blaauwen

Article

Meiresonne et al MBio. 2017 Sep 12;8(5). pii: mBio.01089-

Insert

OmpA-mNG-mCh

Expression

Bacterial

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pYO1125

Plasmid

#235742

Purpose

Protein expression of GST-tagged PX domain (Cys at 2) in bacteria

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

p40 PX domain (NCF4 Human)

Tags

GST-TEV

Expression

Bacterial

Mutation

aa 2-149 with Cys at 2

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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