We narrowed to 18,370 results for: Tec

1. pU6nH1-HD10kb

   Plasmid

   #172657

   Purpose

   Expressing paired pegRNAs from human U6 and H1 promoters to make 10204-bp deletion on HPRT1 gene

   Depositor

   Jay Shendure

   Article

   Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

   Insert

   pegRNA-HD10kbA/pegRNA-HD10kbB
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. TAL2274

   Plasmid

   #36711

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   MDM2-TALEN_Left (Mdm2 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Expression

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pAC1748

   Plasmid

   #127982

   Purpose

   AMA1 plasmid with Aspergillus optimized Cpf1, argB selection marker, and USER cassette (PacI/Nt.BbvCI)

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

   Insert

   Cpf1
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   codon optimized for Aspergillus nidulans

   Promoter

   Aspergillus nidulans tef1 promoter

   Available since

   Dec. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)-FKBP-(GGS)X6-HaloTag C261

   Plasmid

   #171429

   Purpose

   Expression of FKBP-(GGS)X6-HaloTagM C261 in mammalian cells

   Depositor

   Yujie Sun

   Article

   Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

   Insert

   FKBP-(GGS)X6-HaloTag C261
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pH-AFID-3

   Plasmid

   #167361

   Purpose

   For inducing predictable deletions in rice plants

   Depositor

   Caixia Gao

   Article

   Wang et al Nat Biotechnol. 2020 Dec;38(12):1460-146

   Insert

   A3A-Cas9-UDG-T2A-AP lyase
   

   Use

   Tags

   Expression

   Plant

   Mutation

   WT

   Promoter

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAM/CBA-eGFP-miRaSyn(mouse)x3-WPRE-bGHpA

   Plasmid

   #194248

   Purpose

   Expresses 3 miRNAs targeting mouse alpha-Synuclein

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   miRaSynuclein(mouse) (Snca Mouse)
   

   Use

   AAV and RNAi

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pABM-D4-miR30-L1221

   Plasmid

   #132933

   Purpose

   Lentivector for luciferase (control) knockdown and blasticidin resistance

   Depositor

   Jeremy Luban

   Article

   Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

   Insert

   luciferase shRNA
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. EK0354 SFFV-mCherry-SG(Bdnf.s1L72)SG-EGFP (FLP-IN)

   Plasmid

   #191151

   Purpose

   Expression of a 72 bp long sensor for murine Bdnf 3' UTR in mammalian cells

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mCherry:Bdnf.s1L72:EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTH-PanCen TALE-mVenusN

   Plasmid

   #191395

   Purpose

   TALE fused to N-terminal part of split mVenus

   Depositor

   Albert Jeltsch

   Article

   Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

   Insert

   N-terminal part of split mVenus
   

   Use

   TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXCX.CMV.CREB.WPRE

    Plasmid

    #38050

    Depositor

    James Uney

    Article

    Glover et al Neuroreport. 2004 May 19;15(7):1171-5.

    Insert

    CREB (Creb1 Rat)
    

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAPM-D4-miR30-TRIM5

    Plasmid

    #132932

    Purpose

    Lentivector for TRIM5 knockdown and puromycin resistance

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    TRIM5 shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. TAL2384

    Plasmid

    #36793

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    BRCA1-TALEN_Left (Brca1 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pUCBB-ptet-eGFP

    Plasmid

    #32554

    Depositor

    Claudia Schmidt-dannert

    Article

    Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

    Insert

    eGFP (eGFP )
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Mutation

    Promoter

    ptet

    Available since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. p3E-ML1N*2(7Q)

    Plasmid

    #67679

    Purpose

    Multisite gateway entry clone for adding C-terminal fusions of p3E-ML1N*2(7Q)

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    ML1N*2(7Q)
    

    Use

    Gateway multisite 3' entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVH232-Tier1-PmPGK1-mRuby2

    Plasmid

    #169523

    Purpose

    Tier-1 vector encoding PmPGK1-driven mRuby2 expression (PmPGK1-mRuby2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven monomeric red fluorescent protein mRuby2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PPGK

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFPL-Yh2

    Plasmid

    #61650

    Purpose

    Gateway vector for generating yellow fluorescent protein fusions in fungi

    Depositor

    Mark Farman

    Article

    Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

    Insert

    enhanced yellow fluorescent protein
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 10, 2015

    Availability

    Academic Institutions and Nonprofits only

17. fADL-1e-HA-linker-p3

    Plasmid

    #139440

    Purpose

    For cloning and subsequent expression of peptide fragments fused with pIII protein as a part of fd filamentous bacteriophage

    Depositor

    Alexander Gabibov

    Article

    Ishina et al Int J Mol Sci. 2020 Aug 29;21(17). pii:

    Insert

    linker with pelB-leader, cloning site, HA-tag and p3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3.1(+)-HaloTag N58-(GGS)X6-hH2A

    Plasmid

    #171785

    Purpose

    Expression of HaloTag N58-(GGS)X6-hH2A in mammalian cells

    Depositor

    Yujie Sun

    Article

    Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

    Insert

    HaloTag N58-(GGS)X6-hH2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCfB6684

    Plasmid

    #106154

    Purpose

    EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6631, integration into Yarrowia lipolytica chromosomal location IntD_1, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCfB6631

    Plasmid

    #106161

    Purpose

    EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6684 into Yarrowia lipolytica chromosomal location IntD_1, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Insert

    unknown
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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