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LI E-F Tfd

Plasmid

#104520

Purpose

LI terminator module

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

Tfd terminator

Use

Cloning vector

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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puC19 preACT1(del6) short BP-3'SS

Plasmid

#111333

Purpose

T7 transcription

Depositor

Christine Guthrie

Article

Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

Insert

ACT1

Expression

Bacterial

Mutation

shorter branch to 3' splice site distance

Available Since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pOBCol2.3MCSpA

Plasmid

#109485

Purpose

Drive inserted gene in osteoblasts.

Depositor

David Rowe

Article

Kalajzic et al J Bone Miner Res. 2002 Jan;17(1):15-25.

Type

Empty backbone

Tags

None

Expression

Mammalian

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pISH-Nqo1

Plasmid

#105992

Purpose

To prepare riboprobe for in situ hybridization

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Nqo1 (Nqo1 Mouse)

Use

To generate riboprobe

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pSM2

Plasmid

#85753

Purpose

pDONR split marker vector (GenBank accession number: KX904529) for fungal gene deletion. Used with pSM1.

Depositor

Damien Fleetwood

Article

Rahnama et al J Microbiol Methods. 2016 Dec 22. pii: S

Type

Empty backbone

Expression

Bacterial

Promoter

N/A

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pSM1

Plasmid

#85752

Purpose

pDONR split marker vector (GenBank accession number: KX904528) for fungal gene deletion. Used with pSM2.

Depositor

Damien Fleetwood

Article

Rahnama et al J Microbiol Methods. 2016 Dec 22. pii: S

Type

Empty backbone

Expression

Bacterial

Promoter

N/A

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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Ppj220(JBEI-13483)

Plasmid

#89786

Purpose

for expression of C-StrepII-3607

Depositor

Harry Beller

Article

Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

Insert

3607

Tags

StrepII

Expression

Bacterial

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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JPUB_006820

Plasmid

#87921

Purpose

E. coli expression of K. stuttgartiensis protein

Depositor

Harry Beller

Article

Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

Insert

Kuste2803

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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JPUB_006818

Plasmid

#87924

Purpose

E. coli expression of K. stuttgartiensis protein

Depositor

Harry Beller

Article

Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

Insert

Kuste3608

Tags

6xHis and MBP

Expression

Bacterial

Promoter

T7

Available Since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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JPUB_006826

Plasmid

#87923

Purpose

E. coli expression of K. stuttgartiensis protein

Depositor

Harry Beller

Article

Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

Insert

Kuste3608

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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JPUB_006834

Plasmid

#87920

Purpose

E. coli expression of K. stuttgartiensis protein

Depositor

Harry Beller

Article

Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

Insert

Kuste3608

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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JPUB_006828

Plasmid

#87922

Purpose

E. coli expression of K. stuttgartiensis protein

Depositor

Harry Beller

Article

Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

Insert

Kuste2803

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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JPUB_006812

Plasmid

#87925

Purpose

E. coli expression of K. stuttgartiensis protein

Depositor

Harry Beller

Article

Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

Insert

Kuste2803

Tags

6xHis and MBP

Expression

Bacterial

Promoter

T7

Available Since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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psglessClonePX

Plasmid

#87193

Purpose

used for downstream cloning, contains CMV promoter with NheI and XhoI cloning sites flanking a partial insert that gets excised when adding an insert of choice

Depositor

Thomas Cech

Article

Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

Type

Empty backbone

Expression

Mammalian

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pNR292

Plasmid

#79548

Purpose

Output plasmid: contains 16-state register that regulates expression of GFP. Implements RSM with 3-input passcode behavior.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

mTagBFP

Expression

Bacterial

Available Since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Zfhx4

Plasmid

#84324

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Zfhx4. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Zfhx4 (Zfhx4 Mouse)

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Phtf1

Plasmid

#84318

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Phtf1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Phtf1 (Phtf1 Mouse)

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pNR166

Plasmid

#79547

Purpose

Output plasmid: contains 5-state register that regulates expression of GFP. Doesn't behave as expected.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Stmn2

Plasmid

#74397

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Stmn2. Not for gene expression.

Depositor

Peter Mombaerts

Article

Chang et al Gene Expr Patterns. 2016 Jul;21(2):69-80

Insert

stathmin-like 2 (Stmn2 Mouse)

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Tgif1

Plasmid

#74359

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Tgif1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

TGFB-induced factor homeobox 1 (Tgif1 Mouse)

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Zhx1

Plasmid

#74364

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Zhx1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

zinc fingers and homeoboxes 1 (Zhx1 Mouse)

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Six1

Plasmid

#74358

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Six1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

sine oculis-related homeobox 1 (Six1 Mouse)

Available Since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Pbx3

Plasmid

#74352

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Pbx3. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

pre B cell leukemia homeobox 3 (Pbx3 Mouse)

Available Since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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pFA6a-VN155(I153L)-TRP1

Plasmid

#53193

Purpose

chromosomal tagging with Venus

Depositor

John Pringle

Article

Onishi et al J Cell Biol. 2013 Jul 22;202(2):311-29.

Insert

Venus

Use

Yeast genomic targeting

Tags

TRP1

Mutation

aa 1–155, I to L mutation at aa 153

Available Since

May 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGB005

Plasmid

#48822

Purpose

Provides the SV40 nuclear localization signal as GreenGate N-terminal tag module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

SV40 nuclear localization signal

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGA003

Plasmid

#48814

Purpose

Provides the WUS promoter as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

WUS promoter

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pNH052

Plasmid

#42158

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

YFP-RT-YFP

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pNH054

Plasmid

#42160

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mTFP1-RT-mTFP1

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pNH039

Plasmid

#42151

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

CFP

Available Since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pNH058

Plasmid

#42161

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

TAP Tag::mTFP1

Available Since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only

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porcupine

Plasmid

#37377

Depositor

Norbert Perrimon

Article

Kadowaki et al Genes Dev. 1996 Dec 15;10(24):3116-28.

Insert

porcupine (por Fly)

Available Since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only
GFP-Rab40

Plasmid

#31745

Depositor

Ron Vale

Article

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Rab40 (Rab40 Fly)

Tags

GFP

Expression

Insect

Promoter

Actin

Available Since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
pTOPO-rDNA-NTS

Plasmid

#23992

Depositor

Susan Baserga

Article

Gallagher et al Genes Dev. 2004 Oct 15. 18(20):2506-17.

Insert

rDNA NTS

Use

Bacterial cloning - topo

Mutation

None

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
GIMAP4

Plasmid

#25337

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

GIMAP4:G20-M240 (GIMAP4 Human)

Tags

His

Expression

Bacterial

Mutation

Contains amino acids G20-M240

Available Since

Aug. 17, 2010

Availability

Industry, Academic Institutions, and Nonprofits
RICS

Plasmid

#25208

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

RICS:E18-F218 (ARHGAP32 Human)

Tags

His

Expression

Bacterial

Available Since

Aug. 12, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pWZ414-F15

Plasmid

#23064

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H4 deleted amino acids 4 - 19

Available Since

March 3, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F23

Plasmid

#23065

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H4 changed Lysine 5 to Glutamine

Available Since

March 3, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F33

Plasmid

#23068

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H4 changed Lysine 5 to Glutamine H4 K5Q

Available Since

March 3, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F36

Plasmid

#23069

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 changed Lysine 14 to Glycine, H3 K14G

Available Since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F43

Plasmid

#23070

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 changed Lysine 14 to Arginine, H3 K14R

Available Since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only

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