We narrowed to 159,371 results for: Gene

1. NylB'-SCY_pET21b

   Plasmid

   #215417

   Purpose

   Expression construct for NylB'-SCY gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   NylB'-SCY
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   R187S/F264C/D370Y

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRc/RSV Flag MKK3(ala)

   Plasmid

   #14669

   Depositor

   Roger Davis

   Article

   Raingeaud et al Mol Cell Biol. 1996 Mar . 16(3):1247-55.

   Insert

   MKK3(ala) (MAP2K3 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Ser-189Ala Thr-193Ala

   Promoter

   Available since

   July 25, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pmiRGLO Dual-Luciferase-PIK3R1-3'UTR

   Plasmid

   #182252

   Purpose

   Human PIK3R1 3` UTR region containing 1x binding site for miR-150 cloned downstream of luciferase reporter gene

   Depositor

   Silvia Monticelli

   Article

   Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

   Insert

   3' UTR region of PIK3R1 gene
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGM

   Plasmid

   #71250

   Purpose

   luciferase reporter with human GM-CSF promoter -627 to + 28

   Depositor

   Peter Cockerill

   Article

   Cockerill et al Proc Natl Acad Sci U S A. 1999 Dec 21;96

   Insert

   GM-CSF promoter -627 to +28 fragment (CSF2 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   GM-CSF

   Available since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQLink-GST-10xCTD

   Plasmid

   #138472

   Purpose

   Expresses GST-tagged 10xCTD heptapeptide repeat in bacteria

   Depositor

   Britt Glaunsinger

   Article

   Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

   Insert

   Rpb1 (POLR2A Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   10x CTD repeat

   Promoter

   T5

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSW615 - pATHB-8

   Plasmid

   #115990

   Purpose

   entry vector for GreenGate cloning method, promoter

   Depositor

   Sebastien Wolf

   Article

   Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

   Insert

   pATHB-8
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   A1287T to abolish Eco31I site

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. B270 + SPRTN sgSTOP

   Plasmid

   #100718

   Purpose

   B270 plasmid expressing SPRTN sgSTOP (cloned in BbsI site) in combination with ATP1A1 sgSTOP

   Depositor

   Alberto Ciccia

   Article

   Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

   Insert

   sgSTOP targeting SPRTN (cloned using BbsI)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoters

   Available since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLentiCRISPR-v1-sgHK2_2

   Plasmid

   #163461

   Purpose

   lentiviral vector expressing Cas9 and an sgRNA targeting HK2

   Depositor

   Jason Cantor

   Article

   Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

   Insert

   sgRNA 2 targeting HK2 (HK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDNA3-myc3-FBW5

   Plasmid

   #19905

   Depositor

   Yue Xiong

   Article

   Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

   Insert

   F-box and WD repeat domain containing 5 (FBXW5 Human)
   

   Use

   Tags

   myc3

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MCM3APlong_pCSdest

    Plasmid

    #53940

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    MCM3AP long (MCM3AP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6 and CMV

    Available since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

11. pES4/4a

    Plasmid

    #69590

    Purpose

    TCR a chain cDNA in pES4 transgenic construct for creating OT-II mice

    Depositor

    Francis R. Carbone

    Article

    Barnden et al Immunol Cell Biol. 1998 Feb;76(1):34-40.

    Insert

    TCR alpha (Tcra Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H-2Kb

    Available since

    Sept. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

12. plRL118

    Plasmid

    #163632

    Purpose

    Renilla luciferase reporter plasmid; Giles integrating.

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Mycobacterial Optimized Promoter (MOP)

    Available since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHR SFFVp MCP-mCherry IRES h2b-diRFP

    Plasmid

    #102351

    Purpose

    Lentiral expression vector bearing the MS2 Coat Protein (MCP) fused to mCherry as well as the nuclear marker histone 2b fused to two copies of irFP. Also bears the hygromycin selection marker.

    Depositor

    Jared Toettcher

    Article

    Wilson et al Mol Cell. 2017 Sep 7;67(5):757-769.e5. d

    Insert

    MS2 Coat Protein
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV E2F1 E132

    Plasmid

    #24224

    Purpose

    Mammalian expression of human E2F1 (DNA binding mutant) with HA tag

    Depositor

    Kristian Helin

    Article

    Helin et al Genes Dev. 1993 Oct . 7(10):1850-61.

    Insert

    E2F1 E132 (E2F1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

15. LentiCRISPRv2 EGFP sgRNA1

    Plasmid

    #164932

    Purpose

    Expresses EGFP sgRNA1 in mammalian cells

    Depositor

    Ji Luo

    Article

    Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

    Insert

    sgRNA1 targeting Enhanced green fluorescent protein (verified for knockout)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAC91-pmax-dCas9VP64

    Plasmid

    #48223

    Purpose

    dCas9VP64 on pmax expression vector

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP64 activation domain
    

    Use

    CRISPR

    Tags

    HA Tag and VP64

    Expression

    Mammalian

    Mutation

    D10A;H840A (catalytically inactive)

    Promoter

    CAGGS

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. 3xFLAG-CUL7-pCMV7.1

    Plasmid

    #155025

    Purpose

    N-terminal 3xFLAG-tagged CUL7 in a pCMV7.1 vector

    Depositor

    Andre Catic

    Article

    Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

    Insert

    Cullin 7 (CUL7 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    pCMV7.1

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSH1/M-Fv-Casp3-E

    Plasmid

    #15271

    Depositor

    David Spencer

    Article

    Fan et al Hum Gene Ther. 1999 Sep 20. 10(14):2273-

    Insert

    FKBP12(V36)-Caspase3 (CASP3 Human)
    

    Use

    Tags

    HA epitope and Myristoylation-targeting domain c-…

    Expression

    Mammalian

    Mutation

    FKBP12(V36) F36->V

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pVE2_11_p40S_QfDBD_VP16AD_cp_phl_DsRed_0xQUAS

    Plasmid

    #154229

    Purpose

    Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter CP_phl (no QUAS control)

    Depositor

    Arnold Driessen

    Article

    Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.

    Insert

    p40S:QfDBD-VP16AD-GFP-NLS
    

    Use

    Synthetic Biology; Fungal expression

    Tags

    GFP-NLS; DsRed-SKL

    Expression

    Bacterial

    Mutation

    Promoter

    p40S (AN0465 A. nidulans)

    Available since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Empty Target Control

    Plasmid

    #127667

    Purpose

    Encodes for the CRY2-HA and mCherry proteins expressed from the EF1a promoter

    Depositor

    Jennifer Phillips-Cremins

    Article

    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

    Insert

    CRY2-HA-2A-mCherry
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCMV-T7-AcrIIA17_Efa-NLS(SV40) (KAC91)

    Plasmid

    #134333

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA17_Efa with C-terminal NLS (SV40)

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIA17_Efa
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. IFNGR2_pCSdest

    Plasmid

    #53823

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    IFNGR2 (IFNGR2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6 and CMV

    Available since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

23. PDE9A_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176945

    Purpose

    Mammalian expression vector encoding PDE9A and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    PDE9A (PDE9A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBabe puro-eIF4E

    Plasmid

    #33252

    Depositor

    Thomas Roberts

    Article

    Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

    Insert

    eIF4E (EIF4E Human)
    

    Use

    Retroviral

    Tags

    untagged

    Expression

    Mammalian

    Mutation

    unmodified

    Promoter

    SV40

    Available since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

25. ires-eGFP(+HAs) donor-1

    Plasmid

    #87865

    Purpose

    Express eGFP reporter for HDR-induced knock-in

    Depositor

    Bo Feng

    Article

    He et al Nucleic Acids Res. 2016 May 19;44(9):e85

    Insert

    eGFP
    

    Use

    Homologous recombination for gapdh loci

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLJC6-METAP1-3xFLAG

    Plasmid

    #163453

    Purpose

    lentiviral overexpression vector for human METAP1

    Depositor

    Jason Cantor

    Article

    Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

    Insert

    METAP1 (METAP1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    See: comments

    Promoter

    Ubc

    Available since

    March 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRSV-CaMKII(1-290)

    Plasmid

    #45065

    Purpose

    Expression of constit active CaMKII

    Depositor

    Richard Maurer

    Article

    Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

    Insert

    CaM Kinase II (Camk2a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    truncated at amino acid 290

    Promoter

    RSV

    Available since

    July 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. MSCV eIF4E S209D IRES GFP

    Plasmid

    #18765

    Depositor

    Scott Lowe

    Article

    Wendel et al Genes Dev. 2007 Dec 15. 21(24):3232-7.

    Insert

    eIF4E S209D (Eif4e Mouse)
    

    Use

    Retroviral

    Tags

    IRES GFP

    Expression

    Mammalian

    Mutation

    S209D

    Promoter

    Available since

    July 27, 2008

    Availability

    Academic Institutions and Nonprofits only

29. pMflET-o4

    Plasmid

    #101314

    Purpose

    Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and ereB resistance genes, origin of transfer of RP4 plasmid.

    Depositor

    Sébastien Rodrigue

    Article

    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

    Inserts

    oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
    ereB resistance gene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Sufu_pLENTI-CAG-IRES-GFP

    Plasmid

    #176993

    Purpose

    Mammalian lentiviral expression vector encoding Sufu

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    Sufu (Sufu Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. 145-pGL3ctrl-3' UTR

    Plasmid

    #28175

    Depositor

    Michael Kastan

    Article

    Chen et al Genes Dev. 2010 Oct 1. 24(19):2146-56.

    Insert

    Luciferase flanked by tp53 UTRs (TP53 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Note that insert length is based on sum of the 2 …

    Promoter

    Available since

    March 15, 2011

    Availability

    Academic Institutions and Nonprofits only

32. pACM4-ACA

    Plasmid

    #49809

    Purpose

    Encodes fabD, accA, accB, accC, accD in pseudo-operon configuration.

    Depositor

    Mattheos Koffas

    Article

    Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

    Inserts

    ACP transacylase
    Acetyl-CoA carboxylase
    acetyl-CoA carboxylase
    Acetyl-CoA carboxylase
    Acetyl-CoA carboxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Silent site-directed mutagenesis to remove SalI s…

    Promoter

    T7

    Available since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. HsCLYBL(D320A)-FLAG

    Plasmid

    #111291

    Purpose

    mammalian expression

    Depositor

    Vamsi Mootha

    Article

    Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

    Insert

    human CLYBL D320A (CLYBL Human)
    

    Use

    Tags

    linker and FLAG tag: TGGGSGGSGGSMDYKDDDDK

    Expression

    Mammalian

    Mutation

    D320A

    Promoter

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCfB3045(gRNA XI-3)

    Plasmid

    #73287

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-3

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. HR-TERT-promotor

    Plasmid

    #71398

    Purpose

    Homologues recombination donor plasmid fo the wild type TERT promotor region. Includes 1kb of TERT coding region in right homologues arm.

    Depositor

    Thomas Cech

    Article

    Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

    Insert

    TERT (TERT Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Endogenous TERT promotor

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFlox_N-Mau2_Neo_FKBPF36V_mScarlet

    Plasmid

    #163881

    Purpose

    Acute Depletion of Mau2-mScarlet

    Depositor

    Cornelis Murre

    Article

    Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

    Insert

    Mau2 (Mau2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCas9-CAT

    Plasmid

    #80323

    Purpose

    Encodes Cas9 and chloramphenicol acetyltransferase (CAT)

    Depositor

    Sebastian Lourido

    Article

    Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

    Inserts

    Cas9
    Chloramphenicol acetyltransferase
    

    Use

    CRISPR

    Tags

    3X FLAG and NLS

    Expression

    Mutation

    Promoter

    TgTUB1

    Available since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. GBSGFP insulator

    Plasmid

    #121975

    Purpose

    Gli activity reporter, mouse transgenesis

    Depositor

    James Briscoe

    Article

    Balaskas et al Cell. 2012 Jan 20;148(1-2):273-84. doi:

    Insert

    GBS(long)-GFP-insulator (Gnb5 Mouse)
    

    Use

    Mouse transgenesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

39. pT7-EGFP-C1-HsDCP2-E148Q_U

    Plasmid

    #147650

    Purpose

    Mammalian Expression of HsDCP2-E148Q

    Depositor

    Elisa Izaurralde

    Article

    Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

    Insert

    HsDCP2-E148Q (DCP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    two silent mutations compared to the sequence giv…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMIEG3-BCL6

    Plasmid

    #40339

    Depositor

    Alexander Dent

    Article

    Toney et al Nat Immunol. 2000 Sep;1(3):214-20.

    Insert

    BCL6 (BCL6 Human)
    

    Use

    Retroviral

    Tags

    IRES eGFP

    Expression

    Mammalian

    Mutation

    optimized translation initiation codon

    Promoter

    Available since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

41. pSLIK TT FLAG-HA RBFOX2

    Plasmid

    #59769

    Purpose

    doxycycline-inducible expression of FLAG-HA-tagged human RBFOX2 in mammalian cells

    Depositor

    Phil Sharp

    Article

    Jangi et al Genes Dev. 2014 Mar 15;28(6):637-51. doi

    Insert

    RBFOX2 (RBFOX2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    TRE tight

    Available since

    Oct. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. SB51

    Plasmid

    #48184

    Purpose

    Express full length HA tagged p35sr in mammalian cells

    Depositor

    Shoumo Bhattacharya

    Article

    Bhattacharya et al Genes Dev. 1999 Jan 1. 13(1):64-75.

    Insert

    Cited2 (CITED2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

43. pU6-Decoy

    Plasmid

    #80324

    Purpose

    Encodes Cas9 and a CRISPR sgRNA that alleviates toxicity to Cas9 in Toxoplasma gondii

    Depositor

    Sebastian Lourido

    Article

    Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

    Insert

    Decoy sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pLPC-NMYC-mTRF1

    Plasmid

    #64162

    Purpose

    Retroviral vector expressing mouse TRF1 with N-terminal Myc tag

    Depositor

    Titia de Lange

    Article

    Zimmermann et al Genes Dev. 2014 Nov 15;28(22):2477-91. d

    Insert

    mTRF1 (Terf1 Mouse)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

45. pETM6-FAS

    Plasmid

    #49810

    Purpose

    Encodes CnfatB2, fabA, fabH, fabG, fabI in pseudo-operon configuration

    Depositor

    Mattheos Koffas

    Article

    Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

    Inserts

    fatty acyl-ACP thioesterase
    hydroxydecanoyl-ACP dehydratase
    b-ketoacyl-ACP synthase III
    3-ketoacyl-ACP reductase
    enoyl-(acyl carrier protein) reductase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon-optmized. See supplemental information of …

    Promoter

    T7

    Available since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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46. pAC84-pCR8-dCas9

    Plasmid

    #48218

    Purpose

    dCas9 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A)
    

    Use

    CRISPR; Gateway donor

    Tags

    HA Tag

    Expression

    Mutation

    D10A;H840A

    Promoter

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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47. ACE2(-202)-luc

    Plasmid

    #31112

    Purpose

    Luciferase reporter construct for human ACE2 promoter

    Depositor

    Gerhart Ryffel

    Article

    Senkel et al Biochim Biophys Acta. 2005 Dec 20. 1731(

    Insert

    ACE2 promoter (ACE2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    promoter region -202 to +103

    Promoter

    Available since

    Oct. 14, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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48. pRSV-CaMKIV

    Plasmid

    #45062

    Purpose

    Expression of CaMKIV

    Depositor

    Richard Maurer

    Article

    Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

    Insert

    Cam Kinase IV (Camk4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    *

    Promoter

    RSV

    Available since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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49. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-A10

    Plasmid

    #196085

    Purpose

    Inducible CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Insert

    gRNA targeting Adam10
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Cloned using SapI sites - destroyed after inserti…

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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50. mOtop1_pGEMHE

    Plasmid

    #114674

    Purpose

    This construct was used to express mouse Otop1 in Xenopus laevis oocytes

    Depositor

    Emily Liman

    Article

    Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

    Insert

    Otopetrin 1 (Otop1 Mouse)
    

    Use

    For generating the mrna, which can highly express…

    Tags

    Expression

    Mutation

    SNP (rs49325632): Glycine 396 to Alanine

    Promoter

    T7

    Available since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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