We narrowed to 22,247 results for: Sis
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Plasmid#158201PurposeCassette of 10 different binding sites with high affinity to QB phage coat protein, as extracted from the oligo pool experiment.DepositorInsert10 binding sites of QB based on oligo library experiment
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-17
Plasmid#160717PurposeTrpC Terminator bordered by BsaI restriction sitesDepositorInserttryptophan synthase transcription terminator (TtrpC) bordered by BsaI restriction sites
UseSynthetic BiologyTagsExpressionMutationIncorporation of BsaI restriction sitesPromoterAvailable sinceMarch 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-15
Plasmid#160715PurposeFor C-terminal tags with Trichoderma optimised Synthetic 3X-FLAG peptide bordered by BsaI restriction sitesDepositorInsertTrichoderma optimised Synthetic 3X-FLAG peptide
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-3
Plasmid#160703Purposetef1-alpha promoter from Trichoderma virens bordered by BsaI restriction sitesDepositorInserttef1-alpha
UseSynthetic BiologyTagsExpressionMutationIncorporation of BsaI restriction sitesPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRWJB006
Plasmid#160113PurposeDestination vector expressing plant-codon-optimized Cas9 under 35S promoter (BamHI and NotI), with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene freeDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterial and PlantMutationPromoter35SAvailable sinceFeb. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRWJB004
Plasmid#160112PurposeDestination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter (BamHI and NotI), with gRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterial and PlantMutationPromoterUBQ10Available sinceFeb. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK528
Plasmid#155388PurposedCas12a oscillator, reverse crRNAs + PA4-mVenus. (Integration of 511; pOSIP)DepositorInsertsdCas12a (F. novicida)
PA4-mVenus
dCas12a oscillator crRNAs
UseCRISPRTagsExpressionBacterialMutationD917A (nuclease-deactivating)PromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mEGFP-HO-G
Plasmid#164163PurposeExpresses mEGFP (monomeric EGFP with the A206K mutation) under the control of CAG promoter, codon-optimized for Homo sapiens, basic vector, multiclonig site at C terminalDepositorInsertmEGFP A236K
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
p8115 LentiCRISPR v2 sgPTPN14-3
Plasmid#163314PurposeExpresses Cas9 and a human PTPN14-targeting control guide RNADepositorInsertsgPTPN14-3
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
HJP-178-IVS-Syn21-CreAB-NrdJ-1N-WPREw
Plasmid#162473PurposeThis insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.DepositorInsertCreAB
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
HJP-208-IVS-Syn21-CreAB-NrdJ-1N-WPREUw
Plasmid#162478PurposeThis insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.DepositorInsertCreAB
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
HJP-195-IVS-Syn21-gp41-1C-CreB-NrdJ-1N-WPREUw
Plasmid#162476PurposeThis insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.DepositorInsertCreB
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
HJP-194-IVS-Syn21-CreA-gp41-1N-WPREUw
Plasmid#162475PurposeThis insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.DepositorInsertCreA
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
HJP-179-IVS-Syn21-NrdJ-1C-CreC-WPREw
Plasmid#162472PurposeThis insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.DepositorInsertCreC
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
HJP-176-IVS-Syn21-CreA-gp41-1N-WPREw
Plasmid#162470PurposeThis insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.DepositorInsertCreA
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
HJP-196-IVS-Syn21-NrdJ-1C-CreC-WPREUw
Plasmid#162477PurposeThis insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.DepositorInsertCreC
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
HJP-180-IVS-Syn21-gp41-1C-CreB-NrdJ-1N-WPREw
Plasmid#162471PurposeThis insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.DepositorInsertCreB
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtS1HR1AtR2NtB2
Plasmid#160890PurposeExpression of N. tabacum Rubisco small subunit (rbcS-SH1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-S1H
UseTags6xHisExpressionBacterialMutationPromoterrbcS-SH1: T7, chaperones: T13Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtT4aR1AtR2NtB2
Plasmid#160887PurposeExpression of N. tabacum Rubisco small subunit (rbcS-T4a), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-T4a
UseTagsExpressionBacterialMutationPromoterrbcS-T4a: T7, chaperones: T10Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE669
Plasmid#153235PurposeRecipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceOct. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDUAL-Pnmt1-Ost4-GFP
Plasmid#154368PurposeYeast expression of Ost4DepositorInsertOst4 (ost4 Fission Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE672
Plasmid#153238PurposeRecipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAsDepositorTypeEmpty backboneUseCRISPRTagsGFPExpressionPlantMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET15-His-∆NGlpG(Y205F)
Plasmid#155132PurposeExpresses N-terminally His-tagged E. coli GlpG Y205F variant (residues 87-276) from pET15bDepositorInsertGlpG (glpG Escherichia coli)
UseTagsHisExpressionBacterialMutationY205F, insert codes for GlpG residues 87-276PromoterT7Available sinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE473
Plasmid#153264PurposeM1E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE500
Plasmid#153273PurposeM6E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE502
Plasmid#153275PurposeM8E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-Myosin7B-sgRNA
Plasmid#154861PurposeLentiviral expression of Cas9 and sgRNA targeting Myosin7BDepositorInsertMYO7B sgRNA (MYO7B Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJuly 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMAL-C2X dAmp/Kana+-2MT
Plasmid#154353PurposeBacterial expression of 2MTDepositorInsert2MT
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDUAL-P41nmt1-Sad1-GFP
Plasmid#154366PurposeYeast expression of Sad1DepositorInsertSad1 (sad1 Fission Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDUAL-P41nmt1-Nup124-GFP
Plasmid#154364PurposeYeast expression of Nup124DepositorInsertNup124 (nup124 Fission Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDUAL-Pnmt1-Ost4-GFP-MTalpha
Plasmid#154370PurposeYeast expression of Ost4DepositorInsertOst4 (ost4 Fission Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDUAL-P41nmt1-Sad1-GFP-MTn
Plasmid#154367PurposeYeast expression of Sad1DepositorInsertSad1 (sad1 Fission Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1-tglA(C51)HIB
Plasmid#134675PurposeExpresses His6-TglA(C51), TglH, TglI and TglB in E. ColiDepositorInsertsTglA(C51)
TglH
TglI
TglB
UseTagsHis6ExpressionBacterialMutationinsertion of cysteine 51PromoterT7Available sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFloat.assemble(AB).LC_v1
Plasmid#133083PurposeTier 1 destination vector for assembling gene cassettes intended for expression in bacteria. AB sites. Low copy. Version 1.0.DepositorTypeEmpty backboneUseDestination vectorTagsExpressionBacterialMutationPromoterAvailable sinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFloat.assemble(AB).HC_v1
Plasmid#133084PurposeTier 1 destination vector for assembling gene cassettes intended for expression in bacteria. AB sites. High copy. Version 1.0.DepositorTypeEmpty backboneUseDestination vectorTagsExpressionBacterialMutationPromoterAvailable sinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCB6-Occludin
Plasmid#136637PurposeExpression of Occludin in mammalian cellsDepositorInsertOccludin (OCLN Chicken)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCB6-HAOccludin
Plasmid#136638PurposeExpression of HAOccludin in mammalian cellsDepositorInsertHAOccludin (OCLN Chicken)
UseTagsHA (MQDLPGNDNSTAG)ExpressionMammalianMutationPromoterAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCB6-OccludinCT3
Plasmid#136639PurposeExpression of OccludinCT3 in mammalian cellsDepositorInsertOccludinCT3 (OCLN Chicken)
UseTagsExpressionMammalianMutationDeletion of C-terminal cytoplasid domainPromoterAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCB6-HAOccludinCT3
Plasmid#136640PurposeExpression of HAOccludinCT3 in mammalian cellsDepositorInsertHAOccludinCT3 (OCLN Chicken)
UseTagsHA (MQDLPGNDNSTAG)ExpressionMammalianMutationDeletion of C-terminal cytoplasid domainPromoterAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pY71-tet.I.-1
Plasmid#133539PurposesfGFP driven by a T7 promoter with the tetO sequence 36 bp upstream from the promoter start siteDepositorInsertsfGFP
UseTagsStrep tag and TEV siteExpressionBacterialMutationWTPromoterT7Available sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pY71-tet.I.25
Plasmid#133538PurposesfGFP driven by a T7 promoter with the tetO sequence 28 bp downstream from the promoter start siteDepositorInsertsfGFP
UseTagsStrep tag and TEV siteExpressionBacterialMutationWTPromoterT7Available sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pY71-tet.I.20
Plasmid#133537PurposesfGFP driven by a T7 promoter with the tetO sequence 23 bp downstream from the promoter start siteDepositorInsertsfGFP
UseTagsStrep tag and TEV siteExpressionBacterialMutationWTPromoterT7Available sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pY71-tet.I.14
Plasmid#133535PurposesfGFP driven by a T7 promoter with the tetO sequence 17 bp downstream from the promoter start siteDepositorInsertsfGFP
UseTagsStrep tag and TEV siteExpressionBacterialMutationWTPromoterT7Available sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pY71-tet.I.13
Plasmid#133534PurposesfGFP driven by a T7 promoter with the tetO sequence 16 bp downstream from the promoter start siteDepositorInsertsfGFP
UseTagsStrep tag and TEV siteExpressionBacterialMutationWTPromoterT7Available sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pY71-tet.I.12
Plasmid#133533PurposesfGFP driven by a T7 promoter with the tetO sequence 15 bp downstream from the promoter start siteDepositorInsertsfGFP
UseTagsStrep tag and TEV siteExpressionBacterialMutationWTPromoterT7Available sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pY71-tet.I.11
Plasmid#133532PurposesfGFP driven by a T7 promoter with the tetO sequence 14 bp downstream from the promoter start siteDepositorInsertsfGFP
UseTagsStrep tag and TEV siteExpressionBacterialMutationWTPromoterT7Available sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pY71-tet.I.10
Plasmid#133531PurposesfGFP driven by a T7 promoter with the tetO sequence 13 bp downstream from the promoter start siteDepositorInsertsfGFP
UseTagsStrep tag and TEV siteExpressionBacterialMutationWTPromoterT7Available sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pY71-tet.I.9
Plasmid#133530PurposesfGFP driven by a T7 promoter with the tetO sequence 12 bp downstream from the promoter start siteDepositorInsertsfGFP
UseTagsStrep tag and TEV siteExpressionBacterialMutationWTPromoterT7Available sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pY71-tet.I.7
Plasmid#133529PurposesfGFP driven by a T7 promoter with the tetO sequence 10 bp downstream from the promoter start siteDepositorInsertsfGFP
UseTagsStrep tag and TEV siteExpressionBacterialMutationWTPromoterT7Available sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pY71-tet.I.6
Plasmid#133528PurposesfGFP driven by a T7 promoter with the tetO sequence 9 bp downstream from the promoter start siteDepositorInsertsfGFP
UseTagsStrep tag and TEV siteExpressionBacterialMutationWTPromoterT7Available sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only