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MSCV-IRES-GFP ASXL1 (p.G646 Wfs *12) 3x FLAG

Plasmid

#81021

Purpose

Cancer-associated ASXL1 truncation mutant in retroviral vector

Depositor

Anjana Rao

Article

Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

Insert

ASXL1 (ASXL1 Human)

Tags

3X FLAG

Expression

Mammalian

Mutation

p.G646 Wfs *12 truncation

Promoter

5’LTR

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA-FRT/T0-eGFPnls-53BP1 1220-1631 WT

Plasmid

#60814

Purpose

mammalian expression vector

Depositor

Daniel Durocher

Article

Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

Insert

53BP1 (TP53BP1 Human)

Tags

eGFP-NLS (SV40)

Expression

Mammalian

Available Since

April 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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TFORF3072

Plasmid

#144548

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

NELFE (NELFE Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-T2A-Puro_h53BP1_gRNA_D

Plasmid

#110302

Purpose

Expresssion of Cas9-T2A-puromycin resistant gene and a gRNA targeting exon 10 of human 53BP1

Depositor

Chris Kok-Lung Chan

Article

Tiwari et al Nat Commun. 2018 Feb 14;9(1):677. doi: 1

Insert

p53-binding protein 1 (TP53BP1 Human)

Expression

Mammalian

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV6-XL4 ASXL1 (p.G646 Wfs *12) 3x FLAG

Plasmid

#74245

Purpose

Cancer-associated ASXL1 truncation mutant in mammalian expression vector

Depositor

Anjana Rao

Article

Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

Insert

ASXL1 (ASXL1 Human)

Tags

3X FLAG

Expression

Mammalian

Mutation

p.G646 Wfs *12 truncation

Promoter

CMV

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only
pOGS539_rPD-L1nb

Plasmid

#226717

Purpose

Plasmid enabling yeast-mediated expression and secretion of recombinant PD-L1 nanobody (rPD-L1nb)

Depositor

Gautam Dantas

Article

Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

Insert

Recombinant PD-L1 nanobody

Tags

HA tag, Histidine tag, and Mating factor alpha se…

Expression

Yeast

Promoter

pTEF1

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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shRNA Caspase 1 (pSicoR mCherry)

Plasmid

#53575

Purpose

shRNA against human pro-caspase 1

Depositor

Warner Greene

Article

Doitsh et al Nature. 2014 Jan 23;505(7484):509-14. do

Insert

shRNA Caspase 1 (CASP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6 for shRNA; EF1a for mCherry

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only
pLX304-YAP1 _TA

Plasmid

#59143

Purpose

YAP1 mutants that harbor a deletion of the transcriptional activation domain

Depositor

William Hahn

Article

Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

TA (transactivation) domain deleted; aa291-497

Promoter

CMV

Available Since

Oct. 23, 2014

Availability

Academic Institutions and Nonprofits only
pQCXIP mScarlet-MOSPD2 RD/LD

Plasmid

#186476

Purpose

Expression of human MOSPD2 (mutant R404D/L406D, unable to bind FFAT motifs) fused to mScarlet in mammalian cells

Depositor

Catherine Tomasetto

Article

Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Use

Retroviral

Tags

mScarlet

Expression

Mammalian

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-KIR2DS1-COMP5AP-AviTag-9xHis

Plasmid

#157239

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KIR2DS1 (KIR2DS1 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pInducer-TAZ-S89A/S311A

Plasmid

#213589

Purpose

Doxycycline inducible expression of TAZ-S89A/S311A cDNA in mammalian cells

Depositor

Channing Der

Article

Edwards et al Cancer Res. 2023 Nov 7. doi: 10.1158/000

Insert

TAZ (WWTR1 Human)

Use

Lentiviral

Tags

2xHA

Expression

Mammalian

Mutation

S89A, S311A

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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TFORF3184

Plasmid

#144660

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

HSBP1 (HSBP1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-BRD9-PGK-Puro-IRES-GFP

Plasmid

#75114

Purpose

Retroviral expression plasmid encoding wild type human BRD9

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

BRD9 (BRD9 Human)

Use

Retroviral

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only
pBABE-puro-Halo-hMRE11(DB1Δ/DB2Δ)

Plasmid

#208395

Purpose

To generate stable cell line expressing Halo-human MRE11(DB1Δ/DB2Δ) by Retroviral infection.

Depositor

Gaorav Gupta

Article

Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

Insert

Human MRE11 (MRE11 Human)

Use

Luciferase and Retroviral

Expression

Mammalian

Mutation

DB1delta/DB2delta

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pLX304-YAP1 _60-89

Plasmid

#59144

Purpose

YAP1 mutants that harbored a deletion of amino acide at 60-89 position, which disrupts binding to TEAD transcription factors

Depositor

William Hahn

Article

Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

aa60-89 deleted

Promoter

CMV

Available Since

Aug. 19, 2014

Availability

Academic Institutions and Nonprofits only
ACTB-(A)1x

Plasmid

#112055

Purpose

ACTB mRNA tagged with 1 copy of Riboglow (variant A)

Depositor

Amy Palmer

Article

Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

Insert

ACTB (ACTB Human)

Tags

Riboglow RNA tag

Expression

Mammalian

Mutation

1 copy of Riboglow tag (variant A) after stop cod…

Promoter

CMV

Available Since

June 29, 2018

Availability

Academic Institutions and Nonprofits only

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pLX304-YAP1 _PDZ

Plasmid

#59147

Purpose

YAP1 mutants that harbored a deletion of the 5 amino acids

Depositor

William Hahn

Article

Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

PDZ binding domain (last 5aa) deleted

Promoter

CMV

Available Since

Oct. 23, 2014

Availability

Academic Institutions and Nonprofits only
Circular 200,100 (GAPDH)

Plasmid

#170120

Purpose

AAV vector carrying a guide RNA targeting the human GAPDH mRNA

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA (GAPDH Human)

Use

AAV

Expression

Mammalian

Promoter

Human U6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-IRES-GFP ASXL1 (1-479) 3x FLAG

Plasmid

#81023

Purpose

Cancer-associated ASXL1 truncation mutant in retroviral vector

Depositor

Anjana Rao

Article

Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

Insert

ASXL1 (ASXL1 Human)

Tags

3X FLAG

Expression

Mammalian

Mutation

Contains ASXL1 amino acids 1-479

Promoter

5’LTR

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only
TFORF0659

Plasmid

#143233

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

CHD5 (CHD5 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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