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pAAV-hSyn-DIO-FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1

Plasmid

#220609

Purpose

Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory hM4D(Gi) DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1

Use

AAV and Cre/Lox

Tags

FLAG; ArgiNLS

Expression

Mammalian

Promoter

hSyn

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINOH11-A10

Plasmid

#184278

Purpose

Expresses nanobody H11-A10 in E. coli

Depositor

Ray Owens

Article

Mikolajek et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

Insert

Nanobody H11-A10

Tags

His6

Expression

Bacterial, Insect, and Mamm…

Promoter

T7

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINO_H11-B5

Plasmid

#184279

Purpose

Expresses nanobody H11-B5 in E. coli

Depositor

Ray Owens

Article

Mikolajek et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

Insert

Nanobody H11-B5

Tags

His6

Expression

Bacterial, Insect, and Mamm…

Promoter

T7

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINO_11-H6

Plasmid

#184280

Purpose

Expresses nanobody H11-H6 in E. coli

Depositor

Ray Owens

Article

Mikolajek et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

Insert

Nanobody H11-H6

Tags

His6

Expression

Bacterial, Insect, and Mamm…

Promoter

T7

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINH11-H4 Q98R_H100E

Plasmid

#184281

Purpose

Expresses nanobody H11-H4 Q98R_H100E in E. coli

Depositor

Ray Owens

Article

Mikolajek et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

Insert

Nanobody H11-H4 Q98R_H100E

Tags

His6

Expression

Bacterial, Insect, and Mamm…

Promoter

T7

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCVD293

Plasmid

#159765

Purpose

Expression vector for mutated Fc-fused ACE2(740)

Depositor

Jim Wells

Article

Glasgow et al Proc Natl Acad Sci U S A. 2020 Nov 10;11

Insert

ACE2 (ACE2 Human)

Expression

Mammalian

Mutation

K31F/H34I/E35Q

Available Since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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pInducer10-SAE1_sh3

Plasmid

#168985

Purpose

Expresses RFP cDNA and SAE1 shRNA 3 in mammalian cells

Depositor

Ji Luo

Article

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

SUMO1 Activating Enzyme Subunit 1 (SAE1 Human)

Expression

Mammalian

Promoter

Ubc promoter

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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MSCVhyg-HA-FLAG_Ubc9_C93A

Plasmid

#164937

Purpose

Expresses FLAG & HA tagged human Ubiquitin Conjugating Enzyme 9 (UBC9) _C93A mutant cDNA

Depositor

Ji Luo

Article

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

human UBC9 (UBE2I Human)

Use

Retroviral

Tags

FLAG & HA tags

Promoter

PGK promoter

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pBOB-N-3HA-GK_v2

Plasmid

#134277

Purpose

Lentivector encoding 3XHA-tagged GK (isoform 2)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk (Gk Mouse)

Use

Lentiviral

Tags

3X HA

Expression

Mammalian

Mutation

553 amino acids, isoform 2

Promoter

CMV

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-N-Flag-GK_v1

Plasmid

#134270

Purpose

Lentivector encoding Flag-tagged GK (isoform 1)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk (Gk Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk isoform 1; 524 amino acids

Promoter

CMV

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pMBP-parallel1-GK5_v2 (D443N)

Plasmid

#134293

Purpose

Expresses GK5 (isoform 2)-D443N mutant in bacteria

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Tags

MBP

Expression

Bacterial

Mutation

changed Aspartic Acid 443 to Asparagine

Promoter

T7

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pMBP-parallel1-GK5_v2 (D280N)

Plasmid

#134290

Purpose

Expresses GK5 (isoform 2)-D280N mutant in bacteria

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Tags

MBP

Expression

Bacterial

Mutation

changed Aspartic Acid 280 to Asparagine

Promoter

T7

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pMBP-parallel1-GK5_v2 (D30N)

Plasmid

#134289

Purpose

Expresses GK5 (isoform 2)-D30N mutant in bacteria

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Tags

MBP

Expression

Bacterial

Mutation

changed Aspartic Acid 30 to Asparagine

Promoter

T7

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA6-C-Flag-GK5_v1

Plasmid

#134281

Purpose

Expresses Flag-tagged GK5 (isoform 1) in mammalian cells

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 isoform 1; 516 amino acids

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA6-GK5_v2

Plasmid

#134280

Purpose

Expresses GK5 (isoform 2) in mammalian cells

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Expression

Mammalian

Mutation

mouse Gk5 isoform 2; 534 amino acids

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA6-GK5_v1

Plasmid

#134279

Purpose

Expresses GK5 (isoform 1) in mammalian cells

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Expression

Mammalian

Mutation

mouse Gk5 isoform 1; 516 amino acids

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-N-Flag-GK_v2

Plasmid

#134271

Purpose

Lentivector encoding Flag-tagged GK (isoform 2)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk (Gk Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk isoform 2; 553 amino acids

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-C-3HA-GK5_v2

Plasmid

#134269

Purpose

Lentivector encoding 3XHA-tagged GK5 (isoform 2)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

3X HA

Expression

Mammalian

Mutation

mouse Gk5 isoform 2; 534 amino acids

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-C-3HA-GK5_v1

Plasmid

#134268

Purpose

Lentivector encoding 3XHA-tagged GK5 (isoform 1)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

3X HA

Expression

Mammalian

Mutation

mouse Gk5 isoform 1; 516 amino acids

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-C-Flag-GK5_v2

Plasmid

#134260

Purpose

Lentivector encoding Flag-tagged GK5 (isoform 2)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 isoform 2; 534 amino acids

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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