We narrowed to 24,466 results for: nes

1. pGEX-4T-1 3xHA-MKK4-EE

   Plasmid

   #47577

   Purpose

   Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK4

   Depositor

   Kevin Janes

   Article

   Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

   Insert

   MKK4-EE (MAP2K4 Human)
   

   Use

   Tags

   3xHA and GST

   Expression

   Bacterial

   Mutation

   Lacks the first 36 residues of the RefSeq protein…

   Promoter

   Available since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pAAV-FLEX-SaCas9-U6-sgGria3

   Plasmid

   #124855

   Purpose

   Mutagenesis of Gria3

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gria3 (Gria3 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEN_TTmiRc2_3xflag_NRF2

   Plasmid

   #136527

   Purpose

   Used as a donor vector containing N-term 3xFLAG to clone into pSLIK

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   NRF2 (NFE2L2 Human)
   

   Use

   Entry vector for gateway cloning

   Tags

   3x FLAG

   Expression

   Mutation

   Promoter

   Available since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-FLEXfrt-SaCas9-U6-sgGrin1

   Plasmid

   #124865

   Purpose

   Mutagenesis of Grin1

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Grin1 (Grin1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pF712-pET28a-Hs-delta44-GCDH-NHis wt

   Plasmid

   #85113

   Purpose

   expression of recombinant human mature (delta44) GCDH wt with N-terminal 6xHis-tag

   Depositor

   Pal Falnes

   Article

   Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

   Insert

   human mature (delta44) GCDH (GCDH Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   silent SNP: G1041T

   Promoter

   T7

   Available since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEN_TT 3xFLAG-MAVS

   Plasmid

   #158628

   Purpose

   Gateway entry vector for an inducible 3xFLAG-tagged MAVS

   Depositor

   Kevin Janes

   Article

   Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

   Insert

   MAVS (MAVS Human)
   

   Use

   Gateway entry vector

   Tags

   3XFLAG

   Expression

   Mutation

   Promoter

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLX302 ID2-V5 puro

   Plasmid

   #83096

   Purpose

   Lentiviral vector for constitutive expression of human ID2 with C-terminal V5 tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   ID2 (ID2 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

8. TET-pLKO.1 PURO shID2 #2

   Plasmid

   #83087

   Purpose

   Lentiviral shRNA vector for inducible knockdown of human ID2

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   shID2 (ID2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

9. pTRF.1 udsVenus (P_JUND)

   Plasmid

   #58692

   Purpose

   A rapidly responsive reporter of endogenous JUND promoter activity (Lentiviral expression vector for reporter ultradestabilized Venus)

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

   Insert

   endogenous promoter of JUND (JUND Human)
   

   Use

   Lentiviral

   Tags

   udsVenus

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pAAV-TRE-DIO-tdTomato-f

    Plasmid

    #127092

    Purpose

    An AAV genome with tet-inducible, Cre-dependent expression of farnesylated (f) tdTomato

    Depositor

    Viviana Gradinaru

    Article

    Rajendran et al Nat Commun. 2019 Apr 26;10(1):1944. doi:

    Insert

    tdTomato-f
    

    Use

    AAV

    Tags

    farnesylation signal from c-Ha-Ras

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-hSyn-SpdCas9-W3SL

    Plasmid

    #210708

    Purpose

    This Plasmid express hSyn promoter driven SpdCas9

    Depositor

    Isaac Hilton

    Article

    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

    Insert

    S. Pyogenes dCas9
    

    Use

    AAV and CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A/H840A

    Promoter

    hSyn

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSLIK_hygro_BirA_CDKN1A

    Plasmid

    #136538

    Purpose

    Lentiviral expression vector for an inducible BirA-CDKN1A

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    BirA-CDKN1A (CDKN1A )
    

    Use

    Lentiviral; Destinatioin vector for gateway cloni…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLIK TT 3xFLAG ID2 hygro

    Plasmid

    #98394

    Purpose

    Lentiviral vector for inducible expression of human ID2 with 3xFLAG N-terminal tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    ID2 (ID2 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSLIK JunD-HA neo

    Plasmid

    #58515

    Purpose

    Lentiviral expression vector for an inducible HA-tagged mouse JunD

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    JunD (Jund Mouse)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pF713-pEGFP-N1-Hs-FL-METTL20 wt

    Plasmid

    #85114

    Purpose

    transfection of HeLa cell for transient expression of human full-length METTL20-EGFP fusion

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human full-length METTL20 (METTL20 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. G-GECO1.2-Orai1Y80E

    Plasmid

    #73565

    Purpose

    Green fluorescent reporter of mutant Orai1Y80E-associated calcium influx

    Depositor

    Michael Cahalan

    Article

    Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

    Insert

    G-GECO1.2-Orai1Y80E (ORAI1 Human, Synthetic)
    

    Use

    Tags

    G-GECO1.2

    Expression

    Mammalian

    Mutation

    changed tyrosine 80 of Orai1 to glutamate

    Promoter

    CMV Immediate Early

    Available since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pF708-pET28a-Hs-delta38-METTL20-NHis wt

    Plasmid

    #85109

    Purpose

    expression of recombinant human mature (delta38) METTL20 wt with N-terminal 6xHis-tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human mature (delta38) METTL20 (METTL20 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330-Flag-SpCas9-HF1 (without sgRNA)

    Plasmid

    #92102

    Purpose

    Expression plasmid for human codon-optimized high-fidelity SpCas9-HF1, px330-like backbone (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    3xFlag-NLS-Streptococcus pyogenes Cas9-HF1-NLS
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    Cbh

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGB 35S:dCas9:Tnos (GB1191)

    Plasmid

    #68223

    Purpose

    Transcriptional unit for human codon optimized with mutated (D10A, H840A) and inactivated catalytic domains Cas9 protein plant expression driven by the 35S promoter

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    dCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed; human codon optimis…

    Promoter

    35S

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. TET-pLKO.1 PURO shID2 #1

    Plasmid

    #83086

    Purpose

    Lentiviral shRNA vector for inducible knockdown of human ID2

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    shID2 (ID2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only