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Plasmid#79878PurposeBeta-actin2 promoter driving N-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequencesDepositorInsertCerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
TagsAviExpressionBacterialPromoterBeta-actin2Available SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
mPA-GFP-Farnesyl-5
Plasmid#57133PurposeLocalization: Membrane, Excitation: 400 / 504, Emission: 515 / 517DepositorInsertFarnesyl (HRAS Human)
TagsmPA-GFPExpressionMammalianMutationencodes final 20 aa of NM_001130442.1PromoterCMVAvailable SinceJan. 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDEST51-LRRK2-Y1699C
Plasmid#25084DepositorInsertLRRK2 (LRRK2 Human)
UseGateway CloningTags6XHis and V5ExpressionMammalianMutationTyrosine 1699 mutated to CysteineAvailable SinceJuly 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-puro-kozak-scFv-GCN4-HAtag-msfGFP(V206K)-GBI
Plasmid#205162Purposeexpress scFv-GCN4-HAtag-msfGFP(V206K)-GBIDepositorInsertscFv-GCN4-HAtag-msfGFP(V206K)-GBI
ExpressionBacterial and MammalianPromoterCMVAvailable SinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET22b-PelB-6H-Ag43-160N-sfGFP
Plasmid#172886PurposeDisplay sfGFP protein on E. coli cell surfaceDepositorInsertsTagsHHHHHH and TEV recognition site (ENLYFQG)ExpressionBacterialMutationDeleted 1-160 (N terminal)PromoterT7 PromoterAvailable SinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Anti-GFP [N86/8R]
Plasmid#177572PurposeMammalian Expression Plasmid of anti-GFP (Aequorea victoria). Derived from hybridoma N86/8R.DepositorInsertanti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody
ExpressionMammalianPromoterDual CMVAvailable SinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMito-MtrxRFP2
Plasmid#172324Purposefluorescent biosensor for the redox of mitochondrial specific thioredoxin (Trx2)DepositorInsertmitochondrial expression of human thioredoxin 2, fused with a redox-sensitive RFP through Gly-Ser-rich linker
TagsHis-tag and Mitochondria localization sequenceExpressionMammalianPromoterCMV, SP6Available SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD-bHs
Plasmid#134259PurposeFor L-arabinose inducible bacterial expression of S. typhimurium codon-optimized hyaluronidase (Streptomyces koganeinsis UniProt A0A0U2E2) with N-terminal 6x His tagDepositorInsertHyaluronidase from Streptomyces koganeinsis codon-optimized for Salmonella typhimurium
Tags6x HisExpressionBacterialMutationcodon optimized for Salmonella typhimuriumPromoterAraCAvailable SinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMT-ubb-Avi-Cerulean-Rpl10a
Plasmid#79882PurposeUbiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequencesDepositorInsertCerulean protein fused to zebrafish Rpl10a ribosomal unit (rpl10a Zebrafish)
TagsAviExpressionBacterialPromoterUbiquitinAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
[#GS24 -LV] Syn1-GFP-OMP25
Plasmid#233566PurposeLentiviral construct of mitochondrial localized GFP to be expressed in the donor cells under the Synapsin1 promotorDepositorInserteGFP (eGFP Aequorea victoria, Synthetic)
UseLentiviral and Synthetic BiologyExpressionMammalianAvailable SinceJune 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGP-CAG-Ace2N-4AA-mNeon A122D-WPRE-bGH-polyA
Plasmid#172911PurposeMammalian expression of voltage sensorDepositorInsertAce2N-4AA-mNeon A122D
Tagssoma localization targeting sequenceExpressionMammalianMutationA122DPromoterCAGAvailable SinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
8xNFAT-ZsG-muhCD4
Plasmid#162745PurposeNFAT-driven ZsGreen-1 reporter gene with mutated human CD4 geneDepositorInsert8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (CD4 Human)
UseRetroviralExpressionMammalianMutationHuman CD4 gene with glutamine to tyrosine at posi…Available SinceApril 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
SZ_EC_02
Plasmid#155341PurposeOverexpression of xylose utilization genes of Yarrowia lipolytica using EasyCloneYALI. Cut with NotI to linearize repair fragment for C3 locus, overexpressing XK, XDH, and XRDepositorInsertstef1 promoter - xylulose kinase - pex20 terminator
pyk1 promoter - xylose reductase - pex16 terminator
gapdh promoter - xylitol dehydrogenase - lip2 terminator
UseEasycloneyali insert vectorExpressionYeastPromotergapdh, pyk1, and tef1Available SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCh-KIF1A*-strep
Plasmid#120165PurposeExpresses a chimera of mCherry (fluorescent tag), a KIF1A-based anterograde motor and streptavidinDepositorInsertkinesin family member 1A (Kif1a Mouse)
TagsStreptavidin and mCherryExpressionMammalianMutationTruncated KIF1A: 1-405 aaPromoterCMVAvailable SinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMW41: pET-His-MBP-SUMO-R2Bm-SII
Plasmid#209290PurposeExpresses the R2Bm ORF in E. coliDepositorInsertR2
TagsHis, MBP, StrepII, and bdSUMOExpressionBacterialPromoterT7Available SinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-PEmax SpG
Plasmid#187895PurposeAll-in-one prime editor piggyBac transposon with PEmax, SpG variantDepositorInsertSpCas9_H840A_KK_SpG-MMLV-RT_co-P2A-PAC_dTK
UseCRISPR and Synthetic Biology; Piggybac transposonTagsBPSV40 NLS and c-Myc NLSExpressionMammalianPromoterCAGAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-UbC-Tet1v4-dCas9
Plasmid#232180PurposeTet1v4-dCas9 from Nuñez et al. 2021, in a lentiviral cassette with blasticidin resistance, for expression in mammalian cellsDepositorInsertTet1 catalytic domain - XTEN80 linker - dead Cas9 - 2A - BlastR (TET1 Human, S. pyogenes)
UseLentiviralTagsFLAGExpressionMammalianMutationD10A, H840APromoterhUbCAvailable SinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-HA-HCARD-P2A-mCitrine
Plasmid#228902PurposeFor AAV packaging and Cre-dependent expression of a peripherally restricted DREADDDepositorHas ServiceAAV8Inserthydroxycarboxylic acid receptor 2 (Hcar2 Mouse)
UseAAV and Synthetic BiologyTagsHA and P2A mCitrineExpressionMammalianMutationR108KPromoterChicken Beta ActinAvailable SinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-MyoAAV4E
Plasmid#224452PurposeRep/Cap plasmid for the production of MyoAAV 4E, a muscle-tropic AAV capsid in mice and macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVMutationRGDFNNT insert between amino acids 588 and 589 of…Promoterp41Available SinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(1-110)Cy-
Plasmid#169338PurposeExpression of the LT-Fucci(CA) in mammalian cellsDepositorTagsHaloTag7-Geminin(1/110) and HaloTag9-Cdt(1-110)Cy…ExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RAvailable SinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only