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MSCV-6NBC-GFP-PGK-Puro-Barcode81

Plasmid

#83758

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode81

Use

Retroviral

Mutation

deletion of T in starting ATG of GFP (please see …

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-6NBC-GFP-PGK-Puro-Barcode71

Plasmid

#83748

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode71

Use

Retroviral

Mutation

deletion of AA5-233 in GFP (please see depositor …

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pEF HA-TSAd-CNS

Plasmid

#84558

Purpose

Mammalian expression of TSAd-CNS

Depositor

Anne Spurkland

Article

Granum et al BMC Immunol. 2006 Jul 13;7:15.

Insert

TSAd (SH2D2A Human)

Tags

HA

Expression

Mammalian

Mutation

deletion of exon 7

Promoter

EF1a

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only
pGWB425

Plasmid

#74819

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with T7 (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pDGB1alpha1_SF (GB0106)

Plasmid

#68232

Purpose

Twister plasmid to swap inserts from an alpha2 or alpha2R vector to any omega level vector

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Solanum lycopersicum intergenic region

Use

Synthetic Biology; Unspecified

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pTAct2 (GB0210)

Plasmid

#68191

Purpose

Provides the Arabidopsis thaliana Actin2 gene terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtAct2 terminator

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pPMtb (GB0080)

Plasmid

#68167

Purpose

Provides the S. Lycopersicum metallothionein II-like protein (MTB) gene promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

SlMtb promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pPTctp (GB0083)

Plasmid

#68169

Purpose

Provides the S. Lycopersicum translationally-controlled tumor protein homolog promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

SlTCTP promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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p5'UTRAct2 (GB0193)

Plasmid

#68178

Purpose

Provides the 5'-untranslated region of the Arabidopsis thaliana actin2 gene as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

5'UTR AtAct2

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 STAT1

Plasmid

#67116

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

STAT1 (STAT1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 GTF2A1L

Plasmid

#67096

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

GTF2A1L (GTF2A1L Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 TP53

Plasmid

#67078

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TP53 (TP53 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 IRF9

Plasmid

#67079

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

IRF9 (IRF9 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 E-Cad

Plasmid

#67046

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

E-Cad (CDH1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 TCEB2

Plasmid

#67049

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TCEB2 (ELOB Human)

Use

Cell-free expression

Tags

EGFP and His

Mutation

M6R and Q115K

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 BTF3L4

Plasmid

#67051

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

BTF3L4 (BTF3L4 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pEGB 1alfa1R Tnos:Hygromycin:PNos (GB0235)

Plasmid

#68213

Purpose

Transcriptional unit for Hygromycin resistance gene plant expression

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Hyg

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

Pnos

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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mSTIM1_1-250-CFP

Plasmid

#69552

Purpose

Expresses a STIM1_1-250 fragment mammalian cells

Depositor

Yubin Zhou

Article

Ma et al Nat Commun. 2015 Jul 17;6:7826. doi: 10.

Insert

mSTIM1_1-250

Tags

Cyan Fluorescent Protein (CFP)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2015

Availability

Academic Institutions and Nonprofits only
pDGB1omega1_SF (GB0158)

Plasmid

#68242

Purpose

Twister plasmid to swap inserts from an omega2 or omega2R vector to any alpha level vector

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Solanum lycopersicum intergenic region

Use

Synthetic Biology; Unspecified

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pMyb12 (GB0075)

Plasmid

#68199

Purpose

Provides the CDS of theSolanum lycopersicum Myb12 transcription factor as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Myb12 coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pPAt2S3 (GB0029)

Plasmid

#68162

Purpose

Provides the Arabidopsis thaliana Seed-specific promoter 2S3 as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

At2S3 promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pUPD_Mini35s(-60,UTR) (GB0050)

Plasmid

#68176

Purpose

Provides the CaMV Mini35S Promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

CaMV Mini35S promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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CFP(1-158) in pcDNAI/Amp

Plasmid

#60895

Purpose

This vector attaches CFP(1-158) to the N-terminus of a protein.

Depositor

Catherine Berlot

Article

Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

Insert

CFP(1-158)

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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DIRAS1

Plasmid

#55669

Purpose

expression of mouse DIRAS1

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

DIRAS1 (Diras1 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pVRa04_1617

Plasmid

#49658

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF04_1617

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pET12a-AL-103 Y32F Y96F

Plasmid

#47092

Depositor

Marina Ramirez-Alvarado

Article

DiCostanzo et al J Biol Chem. 2012 Aug 10;287(33):27997-8

Insert

AL-103 Y32F Y96F (IGKV3-20 Human)

Tags

ompT

Expression

Bacterial

Mutation

Y32F Y96F

Promoter

T7

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
pEBB-3XMyc-TRAF2 S327A

Plasmid

#44108

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 S327A (TRAF2 Human)

Expression

Mammalian

Mutation

Serine 327 to Alanine

Promoter

EF1α

Available Since

May 2, 2013

Availability

Academic Institutions and Nonprofits only
Y344C

Plasmid

#24433

Depositor

Ron Vale

Article

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin (KIF5B Human)

Tags

6XHis and GFP

Expression

Bacterial

Mutation

C7S/C65A/C168A/C174S/C294A/C330S/C421A, Y344C

Available Since

June 14, 2010

Availability

Academic Institutions and Nonprofits only
L4C/S330C

Plasmid

#24436

Depositor

Ron Vale

Article

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin (KIF5B Human)

Tags

6XHis and GFP

Expression

Bacterial

Mutation

C7S/C65A/C168A/C174S/C294A/C330S/C421A,, L4C/S3…

Available Since

June 14, 2010

Availability

Academic Institutions and Nonprofits only
K222C

Plasmid

#24438

Depositor

Ron Vale

Article

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin (KIF5B Human)

Tags

6XHis and GFP

Expression

Bacterial

Mutation

C7S/C65A/C168A/C174S/C294A/C330S/C421A, K222C

Available Since

June 14, 2010

Availability

Academic Institutions and Nonprofits only