We narrowed to 13,035 results for: Mpl

1. pspCas9-Klenow-ST2-com-vector

   Plasmid

   #176235

   Purpose

   Vector plasmid for expressing spCas9-Klenow fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-Klenow to enhance expression.

   Depositor

   Baisong Lu

   Article

   Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. MAC_N_CCDC93

   Plasmid

   #209687

   Purpose

   Expresses MAC-tagged CCDC93 in mammalian cells

   Depositor

   Markku Varjosalo

   Article

   Laulumaa et al Nat Struct Mol Biol. 2024 Jun;31(6):925-

   Insert

   CCDC93 (CCDC93 Human)
   

   Use

   Tags

   MAC-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. CFAST-Halotag-ER

   Plasmid

   #214413

   Purpose

   ER localization of CFAST fused to Halotag-ER

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   rat cb5 ERM
   

   Use

   Tags

   CFAST-Halo

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLNHA-C1-HseIF4A3_I

   Plasmid

   #146578

   Purpose

   Mammalian Expression of HseIF4A3

   Depositor

   Elisa Izaurralde

   Article

   Palacios et al Nature. 2004 Feb 19. 427(6976):753-7.

   Insert

   HseIF4A3 (EIF4A3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-hFrataxin-HA

   Plasmid

   #31895

   Depositor

   Gino Cortopassi

   Article

   Shan et al Hum Mol Genet. 2007 Apr 15;16(8):929-41.

   Insert

   Frataxin (FXN Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pCAT009

   Plasmid

   #171635

   Purpose

   Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting B2M and CAG promoter expressing mTagBFP2

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-B2M
   mTagBFP2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDESTmycDDX17

   Plasmid

   #19876

   Depositor

   Thomas Tuschl

   Article

   Landthaler et al RNA. 2008 Oct 31. ():.

   Insert

   DEAD (Asp-Glu-Ala-Asp) box polypeptide 17 (DDX17 Human)
   

   Use

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJAT31

   Plasmid

   #204291

   Purpose

   For inserting a phiC31 attP site. HDR integrant is marked with ie1-DsRed, which is expressed in abdomen and mouthparts. Second U6 promoter allows use of two different gRNAs in two synthesized arms.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   ie1-DsRed
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   ie1

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-3XFLAG-renilla-luciferase-beta-globin-control

   Plasmid

   #184393

   Purpose

   Transient transfection plasmid expressing Renilla-luciferase-beta-globin fusion protein

   Depositor

   Robert Bradley

   Article

   Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

   Insert

   Renilla luciferase
   

   Use

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBK1289-AAV-dCjCas9

    Plasmid

    #223138

    Purpose

    AAV backbone for dCjCas9 (endonuclease dead Cas9 from Campylobacter jejuni) with Cj-gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-SlugCas9-NNG-P2A-puro

    Plasmid

    #214352

    Purpose

    Expresses SlugCas9-NNG and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugCas9-NNG and puromycin resistance genes
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q782R/S888R/L906R/N984S/E1012K/K1016I

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBK1340-LV-DUAL-dGFP-NanoLuc-Puro

    Plasmid

    #223160

    Purpose

    Expression of destabilized GFP (dGFP) and NanoLuc for shortened in vivo half-life

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    dGFP-P2A-NanoLuc
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. mt deltaN20 TFB2MdeltaC3

    Plasmid

    #205062

    Purpose

    TFB2M protein expression

    Depositor

    Smita Patel

    Article

    Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

    Insert

    TFB2M (TFB2M Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Lacks N-terminal 20 aa and C-terminal 3 aa

    Promoter

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. wtBxb1_IVT

    Plasmid

    #222340

    Purpose

    Plasmid for wild type Bxb1 mRNA in vitro transcription template

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    Codon-optimized wtBxb1
    

    Use

    Template for in vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T7 (inactivated)

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFA6a-GFP (F64L, S65T, V163A)-TRP1

    Plasmid

    #53184

    Purpose

    chromosomal tagging with stable GFP

    Depositor

    John Pringle

    Article

    Ko et al Mol Biol Cell. 2007 Dec;18(12):5139-53.

    Insert

    TRP1 (TRP1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    GFP (F64L, S65T, V163A)

    Expression

    Mutation

    Promoter

    Available since

    May 27, 2014

    Availability

    Academic Institutions and Nonprofits only

16. psiCHECK2-let-7 WT

    Plasmid

    #78260

    Purpose

    psiCHECK2 dual luciferase reporter harboring a fully complementary let-7 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene

    Depositor

    Joanne Weidhaas

    Article

    Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10

    Insert

    fully complimentary let-7 target site
    

    Use

    Luciferase; Microrna activity

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. myc-hULK1

    Plasmid

    #31961

    Depositor

    Do-Hyung Kim

    Article

    Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

    Insert

    ULK1 (ULK1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available since

    Sept. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEYFP-Sec24C

    Plasmid

    #66608

    Purpose

    YFP fusion of Sec24C

    Depositor

    David Stephens

    Article

    Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

    Insert

    Sec24C (SEC24C Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. VSVG-mCherry-RUSH

    Plasmid

    #203647

    Purpose

    RUSH cargo: Str-KDEL_IRES_SBP-mCherry-VSVG

    Depositor

    Juan Bonifacino

    Article

    Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

    Insert

    VSVG
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. Flag Nprl2 pRK5

    Plasmid

    #46333

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    Nprl2 (NPRL2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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