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We narrowed to 62,913 results for: cloning

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Showing: 4661 - 4680 of 62913 results

  2. pET-28b-T7-henAsCas12a(E174R/S542R/K548R)-NLS(nucleoplasmin)-6xHis (AAS1885)

    Plasmid
    #114072
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized enAsCas12a(E174R/S542R/K548R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized enAsCas12a (E174R/S542R/K548R) with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    E174R, S542R, and K548R
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    114072-plasmid-map-sequence-id-220522

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  8. pLenti-xBE4Gam-P2A-Puro

    Plasmid
    #112676
    Purpose
    Lentivirus for constitutive expression of xCas9(3.7)-BE4Gam in mammalian cells (codon optimized)
    Depositor
    Lukas Dow
    Publication
    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419
    Insert
    xCas9(3.7)-BE4Gam
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…
    Promoter
    EF1s
    Available since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    112676-plasmid-map-sequence-id-217196

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  11. pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST

    Plasmid
    #109048
    Purpose
    Anion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites under the control of internal ribosome entry sequence in a viral vector
    Depositor
    Hillel Adesnik
    Publication
    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/
    Has service
    AAV9
    Insert
    eGtACR1-ST
    Use
    AAV
    Promoter
    CAG
    Available since
    July 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    109048-plasmid-map-sequence-id-264817

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  12. pCAGGS-ChrimsonR-mRuby2-ST

    Plasmid
    #105447
    Purpose
    Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites
    Depositor
    Hillel Adesnik
    Publication
    Pegard et al Nat Commun. 2017 Oct 31;8(1):1228. doi:
    Insert
    ChrimsonR-mRuby2-ST
    Tags
    mRuby2 and soma targeting motif
    Expression
    Mammalian
    Mutation
    K176R in Chrimson
    Promoter
    pCAGGS
    Available since
    Feb. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    105447-plasmid-map-sequence-id-203780

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Showing: 4661 - 4680 of 62913 results