We narrowed to 11,735 results for: pcdna

1. pcDNA3.1/NDUFS8/myc-His

   Plasmid

   #127489

   Purpose

   Expresses myc-tagged NDUFS8 in mammalian cells

   Depositor

   Dieter Wolf, Shashi Jain

   Article

   Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

   Insert

   NDUFS8 (NDUFS8 Human)
   

   Use

   Tags

   myc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-FRB-VMAc-CSarcin

   Plasmid

   #70223

   Purpose

   Expresses VMA intein with C-terminal portion of alpha-sarcin

   Depositor

   Perry Howard

   Article

   Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

   Insert

   alpha Sarcin (C-terminal portion)
   

   Use

   Tags

   FKBP-rapamycin binding (FRB) domain of the mammal…

   Expression

   Mammalian

   Mutation

   Changed Asp 28 to Cys, deleted amino acids 1-27

   Promoter

   CMV

   Available since

   Nov. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3.1 Myc-huMEKK3 (K391A)

   Plasmid

   #44157

   Depositor

   Xin Lin

   Article

   Blonska et al Mol Cell Biol. 2004 Dec;24(24):10757-65.

   Insert

   MEKK3 (K391A) (MAP3K3 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   K391A, kinase-dead mutant

   Promoter

   Available since

   April 10, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3-sACE2-T92Q-Fc(IgG1)

   Plasmid

   #145170

   Purpose

   Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) glycosylation mutant T92Q

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Fc region of human IgG1

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.2/V5 hsa-mir-142

   Plasmid

   #26309

   Depositor

   David Bartel

   Article

   Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

   Insert

   hsa-mir-142 (MIR142 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pCDNA3 Flag Jip1 JBD

   Plasmid

   #15751

   Purpose

   binds to Jnk

   Depositor

   Roger Davis

   Article

   Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

   Insert

   Jip1 (Jnk binding domain) (Mapk8ip1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   deleted 1-126 and 282-660

   Promoter

   Available since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3-Lyn-sACt-mCherry

   Plasmid

   #138216

   Purpose

   Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; plasma membrane targeted.

   Depositor

   Jin Zhang

   Article

   Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

   Insert

   Lyn-sACt-mCherry (Adcy10 Rat)
   

   Use

   Tags

   N-terminal targeting sequence from Lyn kinase and…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCDNA3 -5'cMyc-SMCl- S957A

   Plasmid

   #32368

   Depositor

   Michael Kastan

   Article

   Kim et al Genes Dev. 2002 Mar 1;16(5):560-70.

   Insert

   SMC1A (SMC1A Human)
   

   Use

   Tags

   c-Myc

   Expression

   Mammalian

   Mutation

   S957A

   Promoter

   Available since

   Sept. 30, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.1-TAT-1-delta2-26-FLAG

   Plasmid

   #35983

   Depositor

   Susana Valente

   Article

   Mousseau et al Cell Host Microbe. 2012 Jul 19;12(1):97-

   Insert

   transactivating protein (tat )
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   tat from HIV1 aa 2-26 deleted

   Promoter

   Available since

   Sept. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.2/V5 mmu-mir-223

    Plasmid

    #26334

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    mmu-mir-223 (Mir223 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1-2xFLAG-2xSTREP-CDK4

    Plasmid

    #172615

    Purpose

    Expresses 2xFLAG-2xSTREP-tagged CDK4 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CDK4 (CDK4 Human)
    

    Use

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CeNL(Ca2+)_1.2μ-pcDNA3

    Plasmid

    #111926

    Purpose

    Cyan color bright luminescent calcium indicator with intermediate affinity

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    CeNL(Ca2+)_1.2μ
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E67D, E104D, D133E at CaM

    Promoter

    CMV

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCDNA3 Flag MKK7a1 (ala)

    Plasmid

    #14539

    Depositor

    Roger Davis

    Article

    Tournier et al Proc Natl Acad Sci U S A. 1997 Jul 8. 94

    Insert

    MKK7a1(Ala) (Map2k7 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser-198Ala Thr-202Ala

    Promoter

    Available since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

14. 3579 pcDNA3 Bad S136A

    Plasmid

    #8798

    Depositor

    Stanley Korsmeyer

    Article

    Harada et al Proc Natl Acad Sci U S A 2001 Aug 14;98(

    Insert

    Bad (Bad Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S136A

    Promoter

    Available since

    July 6, 2005

    Availability

    Academic Institutions and Nonprofits only

15. pcDNAI-GAL4-CREB-S133A

    Plasmid

    #46770

    Purpose

    Expression of GAL4 fusion to CREB-S133A

    Depositor

    Richard Maurer

    Article

    Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

    Insert

    GAL4(1-147)-CREB-S133A (Creb1, GAL4 Rat, Budding Yeast)
    

    Use

    Tags

    GAL4

    Expression

    Mammalian

    Mutation

    Changed CREB serine 133 to Alanine

    Promoter

    CMV

    Available since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3 5' cMyc- SMCI-S966A

    Plasmid

    #32371

    Depositor

    Michael Kastan

    Article

    Kim et al Genes Dev. 2002 Mar 1;16(5):560-70.

    Insert

    SMC1A (SMC1A Human)
    

    Use

    Tags

    c-Myc

    Expression

    Mammalian

    Mutation

    S996A

    Promoter

    Available since

    Sept. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

17. GFP Cav2.2 HA D665A pcDNA3

    Plasmid

    #206113

    Purpose

    expression of rabbit Cav2.2 with a N-terminal GFP tag and an exofacial double HA tag in domain II and a D665A mutation that allows trafficking to the plasma membrane but channel is not functional

    Depositor

    Annette Dolphin

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    D665A

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. cacna2d1 R241A D491A HA pcDNA3

    Plasmid

    #206109

    Purpose

    expression of rat alpha2delta-1 calcium channel auxillary subunit with a single HA tag inserted after aa549 and with R421A and D491A mutations that both disrupt Gabapentin binding

    Depositor

    Annette Dolphin

    Insert

    cacna2d1 (Cacna2d1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R241A D491A

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-FRB-LplA(W37V)

    Plasmid

    #35608

    Depositor

    Alice Ting

    Article

    Slavoff et al J Am Chem Soc. 2011 Dec 14;133(49):19769

    Insert

    FRB-LplA(W37V)
    

    Use

    Tags

    FLAG (internal fusion), HA, and His Tag

    Expression

    Mammalian

    Mutation

    LplA has Trp37 to Val mutation

    Promoter

    CMV

    Available since

    March 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5/FRT/TO GFP DNAJA4

    Plasmid

    #19494

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    DNAJA4 (DNAJA4 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge