We narrowed to 149,122 results for: ins;

1. pORE303N

   Plasmid

   #194438

   Purpose

   CRISPR, Cas9 driven by double 35S, nptII for plant selection, KpnI site for gRNA insertion

   Depositor

   Chung-Jui Tsai

   Article

   Ortega et al New Phytol. 2022 Nov 16. doi: 10.1111/np

   Insert

   Cas9
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   2x35S

   Available Since

   Jan. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFA6a-link-ytdTomato-URA3

   Plasmid

   #168054

   Purpose

   Replaces Addgene plasmid 118461. Yeast tagging vector to create a gene fusion with yeast optimized tdTomato with CaUra3 selection; Contains the linker region at the 5’ end of the FP

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ytdTomato
   

   Tags

   ytdTomato

   Expression

   Yeast

   Available Since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]Myc[open]

   Plasmid

   #71805

   Purpose

   Generating transgenic flies for 4-hydroxytamoxifen-inducible DamID

   Depositor

   Bas van Steensel

   Article

   Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

   Insert

   FL.hsp70P-Dam[4-HT-intein@L127C]-Myc[open]
   

   Expression

   Insect

   Available Since

   March 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. AICSDP-079:DCP1A-mEGFP

   Plasmid

   #193922

   Purpose

   Homology arms and mEGFP-SRA for N-terminus tagging of human DCP1A

   Depositor

   Allen Institute for Cell Science

   Insert

   DCP1A Homology Arms with mEGFP-SRA (DCP1A Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   Dec. 22, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pBAD24-sfGFPx2

   Plasmid

   #51559

   Purpose

   Superfolder GFP ORF cloned into pBAD24 for expression in E. coli. It contains additional aminoacids in the N and C terminus (polilinker sequences for cloning proteins in frame with GFP)

   Depositor

   Sankar Adhya, Francisco Malagon

   Article

   Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

   Insert

   superfolder GFP
   

   Tags

   GLESTCRHASLAVLADERRFSA and MARARA

   Expression

   Bacterial

   Mutation

   Contains additonal aa in the N and C terminus (in…

   Available Since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTP813

   Plasmid

   #113308

   Purpose

   human Mre11 in baculovirus transfer vector

   Depositor

   Tanya Paull

   Article

   Bhaskara et al Mol Cell. 2007 Mar 9;25(5):647-61. doi:

   Insert

   Mre11 (MRE11 Human)
   

   Tags

   Flag

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBLADE_ONLY_A

   Plasmid

   #168051

   Purpose

   Expresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter.

   Depositor

   Barbara Di Ventura

   Article

   Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

   Insert

   Blue light-inducible AraC dimers in Escherichia coli
   

   Expression

   Bacterial

   Promoter

   J23101**

   Available Since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmTurquoise2-N1

   Plasmid

   #60561

   Purpose

   Expresses the cyan fluorescent protein variant mTurquoise2, can be used to create fusion proteins

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   mTurquoise2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFB1_hPMS2

   Plasmid

   #129425

   Purpose

   Expression human PMS2 protein using baculovirus

   Depositor

   Peggy Hsieh

   Article

   Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

   Insert

   human PMS2 (PMS2 Human)
   

   Expression

   Insect

   Promoter

   Polyhedrin promoter

   Available Since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMON-HsNMT1

    Plasmid

    #67475

    Purpose

    Expression of human N-myristoyltransferase 1 for N-myristoylation of ARF family proteins in bacteria

    Depositor

    Richard Kahn

    Article

    Van Valkenburgh et al Methods Enzymol. 2002;344:186-93.

    Insert

    HsNMT1 (NMT1 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

11. nos-Cas9.874Z1

    Plasmid

    #112685

    Purpose

    Express hSpCas9 under nanos promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    hSpCas9
    

    Tags

    3x FLAG, EGFP, and NLS

    Expression

    Insect

    Mutation

    Humanized Cas9 bacterial sequence

    Promoter

    nanos

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFB1_hMLH1

    Plasmid

    #129426

    Purpose

    Expression human MLH1 protein using baculovirus

    Depositor

    Peggy Hsieh

    Article

    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

    Insert

    human MLH1 (MLH1 Human)
    

    Expression

    Insect

    Promoter

    Polyhedrin promoter

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRXG

    Plasmid

    #113643

    Purpose

    This construct contains an amber stop codon within the linker between the two genes. The read through rate of the amber stop codon can be determined using the ratio of GFP signal to RFP signal.

    Depositor

    Jeffrey Barrick

    Article

    Monk et al ACS Synth Biol. 2016 Sep 20.

    Insert

    mRFP1-linker-sfGFP
    

    Use

    Synthetic Biology

    Available Since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDV17_pCMV-MCP-ADAR2dd(E488Q)-NES

    Plasmid

    #200406

    Purpose

    Expresses a compact, engineered version of ADAR2 constitutively in mammalian cells

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    MCP-ADAR2dd(E488Q)-NES
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Mutation

    E488Q (hyperactive)

    Promoter

    CMV

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-DeltaJunD

    Plasmid

    #68547

    Purpose

    AAV expression of DeltaJunD

    Depositor

    Eric Nestler

    Article

    Winstanley et al J Neurosci. 2007 Sep 26;27(39):10497-507

    Insert

    DeltaJunD-IRES-GFP
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    truncated splice variant

    Promoter

    CMV

    Available Since

    July 11, 2018

    Availability

    Academic Institutions and Nonprofits only

16. pSEM253 - sgRNA mosTI hygroR

    Plasmid

    #159827

    Purpose

    Single copy or array insertion by MosTI using split hygroR selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    sgRNA mosTI hygroR
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. AICSDP-166:CDH1-mEGFP

    Plasmid

    #193921

    Purpose

    Homology arms and LE-mEGFP sequence for C-terminus tagging of human cadherin 1

    Depositor

    Allen Institute for Cell Science

    Insert

    cadherin 1 Homology Arms with LE-mEGFP (CDH1 Human)
    

    Use

    AAV and CRISPR; Donor template

    Tags

    linker-mEGFP

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    Dec. 22, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pSEM270 - MCS mosTI hygroR target

    Plasmid

    #159826

    Purpose

    Single copy or array insertion by MosTI using split hygroR selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    MCS mosTI hygroR target
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFA6a-link-ymTq2-URA3

    Plasmid

    #168057

    Purpose

    Replaces Addgene Plasmid 118458. Yeast tagging vector to create a gene fusion with yeast optimized mTurquoise2 with CaUra3 selection; Contains the linker region at the 5’ end of the FP

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymTurquoise2
    

    Tags

    ymTurquoise2

    Expression

    Yeast

    Available Since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSEM321 - array co-injection fragment mosTI hygroR

    Plasmid

    #159828

    Purpose

    Single copy or array insertion by MosTI using split hygroR selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    array co-injection fragment mosTI hygroR
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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