We narrowed to 5,937 results for: plasmid dna
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Plasmid#68554PurposeThis plasmid expresses full length IDAX containing a DNA binding mutation.DepositorInsertIDAX-DBM (Cxxc4 Mouse)
TagsMycExpressionMammalianMutationT162A, H164A and Q165A mutationsPromoterEF1aAvailable SinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
p3-HPRT1-S104R-PdTK-mCh
Plasmid#107276PurposeHPRT1 c.312C>A Munich donor vector with unilateral microhomologyDepositorInsertsMutationc.306G>T, c.312C>AAvailable SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3-HPRT1-S104Rf-PdTK-mCh
Plasmid#107277PurposeHPRT1 c.312C>A (Munich) donor vector with bilateral microhomologyDepositorInsertsMutationc.306G>T, c.312C>AAvailable SinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMT1-13X-gRNA1-2(AtUBI10)-MTAP-LUC
Plasmid#234370PurposeT-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAsDepositorInsertsNbGP41-sfGFP-VP64-GB1
Luciferase
dCas9-13XGP41
gRNA 1-1 and gRNA 1-2
UseSynthetic BiologyTagsGB1, GP41, and sfGFPExpressionPlantPromoterArabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…Available SinceApril 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT1-24X-gRNA1-2(AtUBI10)-MTAP-LUC
Plasmid#234372PurposeT-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAsDepositorInsertsNbGP41-sfGFP-VP64-GB1
Luciferase
dCas9-24XGP41
gRNA 1-1 and gRNA 1-2
UseSynthetic BiologyTagsGB1, GP41, and sfGFPExpressionPlantPromoterArabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…Available SinceApril 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCM100_ PB5'IR-hU6-gRNA-CS1- CAG-tdTomato- PB3'IR
Plasmid#229995PurposePiggyBac sgRNA cloning plasmid with tdTomato reporter with a capture sequence (cs1)DepositorTypeEmpty backboneUseCRISPRTagsNotI site for NEBuilder HiFi DNA Assembly with ss…ExpressionMammalianPromoterCAGAvailable SinceFeb. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPP441
Plasmid#158801PurposePlasmid containing Homo sapiens codon optimized CasΦ-2 and a SapI-GG stuffer spacer for editing in human cells.DepositorInsertCasPhi-2
Tags2xSV40NLS-2xFLAGExpressionMammalianAvailable SinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPP085
Plasmid#158795PurposepRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purificationDepositorInsertCasPhi-2
Tags6xHisExpressionBacterialAvailable SinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPP089
Plasmid#158796PurposepRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-3 in MCS1 for expression in E. coli and protein purificationDepositorInsertCasPhi-3
ExpressionBacterialAvailable SinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPP076
Plasmid#158794PurposepRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-1 in MCS1 for expression in E. coli and protein purificationDepositorInsertCasPhi-1
ExpressionBacterialAvailable SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPP378
Plasmid#158797PurposepRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-1(D371A) in MCS1 for expression in E. coli and protein purificationDepositorInsertdCasPhi-1 (D371A)
ExpressionBacterialAvailable SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPP384
Plasmid#158799PurposepRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-3(D413A) in MCS1 for expression in E. coli and protein purificationDepositorInsertdCasPhi-3 (D413A)
ExpressionBacterialAvailable SinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVC-CRISPR1
Plasmid#120366PurposePlasmid containing shortened version of upstream V-C CRISPR array for integration assaysDepositorInsertCRISPR1
UseCRISPRAvailable SinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVC-CRISPR
Plasmid#120369PurposePlasmid containing shortened versions of both natural V-C CRISPR arrays for preparing sequencing libraryDepositorInsertsCRISPR1
CRISPR2
UseCRISPRAvailable SinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-ARF4-GFP
Plasmid#67392PurposeMammalian expression of hArf4 with C-term GFP tagDepositorInsertARF4 (ARF4 Human)
TagsGFPExpressionMammalianMutationV53A, L123P and M134I mutations were unintended P…PromoterCMVAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-nbOcIgG-DddA11_NT
Plasmid#232441PurposeAnti-rabbit IgG Fc specific nanobody-DddA11_NT for D&Dseq (sc TFs molecular footprint)DepositorInsertnbOcIgG-DddA11_N
Available SinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-nbMmKappa-DddA11_NT
Plasmid#232442PurposeAnti-mouse kappa chain specific nanobody-DddA11_NT for D&Dseq (sc TFs molecular footprint)DepositorInsertnbMmKappa-DddA11_NT
Available SinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKAR1
Plasmid#23104PurposeAllows expression of shRNA from U6 promoter and a rescue cDNA from a doxycycline (Tet) inducible promoter in the same plasmidDepositorTypeEmpty backboneUseRNAiTagsFlagExpressionMammalianPromoterTRE for cDNA; U6 for shRNAAvailable SinceMay 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1328 LV EF1a-CD20 IRES-EGFP
Plasmid#201918Purpose2nd generation lentiviral transfer plasmid for engineering cells to express human CD20DepositorInsertCD20
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-6His-MBP-MpAgo
Plasmid#79249PurposeThis plasmid encodes the Argonaute protein from Marinitoga Piezophila and is codon optimized for E. coli expressionDepositorInsertMarinitoga piezophila Argonaute
UseCRISPRTags6XHis-tag + MBP + PreScission Cleavage SiteExpressionBacterialPromoterT7Available SinceJuly 1, 2016AvailabilityAcademic Institutions and Nonprofits only