We narrowed to 24,999 results for: CHI
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Plasmid#170835PurposeCas9-mediated knockout of Eomes in mammalian cellsDepositorAvailable SinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only
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Eomes gRNA#3
Plasmid#170836PurposeCas9-mediated knockout of Eomes in mammalian cellsDepositorAvailable SinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcry:mCherry,-600unc:cct3GFP
Plasmid#172528PurposealphaA-crystallin promoter (cry) drives mCherry expression in the lens. The 600bp unc-45b muscle-specific promoter drives expression of GFP-tagged zebrafish cct3.DepositorInsertcct3-GFP
ExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-Xlrbpa-dsRBD(+6)
Plasmid#171548PurposeEncoding part of the Xenopus Xlrbpa-2 protein from M120 to L202DepositorInsertXlrbpa (prkra.L Frog)
ExpressionBacterialMutationa six amino acid_TRLTEG_insertion in the L1 regionAvailable SinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-Staufen-dsRBD(+6)
Plasmid#171546PurposeEncoding part of the Drosophila Staufen protein from M571 to P656DepositorInsertStaufen (stau Fly)
ExpressionBacterialMutationa six amino acid_TRLTEG_insertion in the L1 regionAvailable SinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-Staufen
Plasmid#171545PurposeEncoding part of the Drosophila Staufen protein from M571 to P656DepositorInsertStaufen (stau Fly)
ExpressionBacterialAvailable SinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
4LNDD-pCDNA3.1(+)
Plasmid#172227PurposeExpresses human ADAR2 Deaminase Domain (DD) fused with 4 repeats of lambda N peptide (4LN) in mammalian cellsDepositorInsertLambda N peptide - human ADAR2 Deaminase Domain
Tags6xHis and FlagExpressionMammalianPromoterCMVAvailable SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Six1
Plasmid#158532PurposeExpresses mouse Six1 in mammalian cellsDepositorAvailable SinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
YF
Plasmid#171459PurposeExpresses YF, a FiCE of iCMM, in mammalian cellsDepositorInsertEYFP-FKBP12
ExpressionMammalianPromoterCMVAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Mdn1-deltaC
Plasmid#171603PurposeExpresses S. pombe Mdn1 a.a. 1-3911 in insect cells.DepositorInsertMdn1
TagsHis6 tagExpressionInsectMutationC-terminal deletion; a.a. 1-3911 onlyAvailable SinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1693
Plasmid#167595PurposeExpress FepA with His6 tag between residues 393 and 394 of the mature amino acid sequence . Please note that mutation numbers are not based on start codon of insert.DepositorInsertFepA
ExpressionBacterialMutationHis6 tag inserted after residue 393 in the mature…Available SinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-null (BPK1077)
Plasmid#171128PurposePlasmid encoding a pCMV-T7 with a 2 amino acid peptide prior to a stop codon.DepositorInsertpCMV-T7 null expression plasmid that encodes a two amino acid peptide (Met-Asp)
UseCRISPRExpressionBacterial and MammalianPromoterpCMV and T7Available SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-HisTim12_CtoA
Plasmid#170281PurposeExpresses yeast Tim12 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli productionDepositorInsertyeast Tim12 (TIM12 Budding Yeast)
TagsTEV-cleavable N-terminal His tag (on backbone)ExpressionBacterialMutationmutated two non-conserved Cys to AlaAvailable SinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tol2-aACry::EGFP-zebrafish Plaat1 (WT)
Plasmid#168772PurposeExpress EGFP-zebrafish Plaat1 (WT) in zebrafish lensDepositorAvailable SinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tol2-GgVCL/*TBM
Plasmid#162790PurposeMUTATED_transfection of mammalian cells with Vcl mutated in Tankyrase Binding Motif -II (TBM-II) under a β-actin promoter, with IRES-GFP allowing identification of transfected cellsDepositorArticleInsertMutated Gallus gallus vinculin (G454V) (VCL Chicken)
TagsIRES-GFPExpressionBacterial and MammalianMutationchanged Gly 454 to ValPromoterbeta actinAvailable SinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
DT048A
Plasmid#159409PurposeConstitutive LuxR expression, controlled under J23101 E.coli promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoterDepositorInsertpr.J23101-LuxR-pr.LuxR-PFO
UseSynthetic BiologyTagsFLAG/FLAGExpressionBacterialPromoterJ23101 and pLux promoterAvailable SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
DT046A
Plasmid#159407PurposeConstitutive LuxR expression, controlled under E.coli tet promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoterDepositorInsertpr.tet-LuxR-pr.LuxR-PFO
UseSynthetic BiologyTagsFLAG/FLAGExpressionBacterialPromotertet and pLux promoterAvailable SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB.His.MBP.3C CARD8 UPA-STOP
Plasmid#164031PurposeBacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 UPADepositorAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUE1-TSP-ORF68 R14A/K310A
Plasmid#162655PurposeExpresses ORF68 R14A/K310A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage siteDepositorInsertORF68
TagsN-terminal 2xStrep-Tag II and HRV 3C siteExpressionMammalianMutationAmino acids R14 and K310 mutated to alaninePromoterCMVAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-zeo-2xStrep-ORF68 C52A
Plasmid#162660PurposeLentiviral vector for stable expression of N-terminal 2xStrep-tagged ORF68 C52A in mammalian cellsDepositorInsertORF68
UseLentiviralTagsN-terminal 2xStrep-Tag IIExpressionMammalianMutationAmino acid C52 mutated to alaninePromoterCMVAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-2xStrep-ORF68 K450A/R451A
Plasmid#162638PurposeExpresses N-terminal 2x-Strep-tagged ORF68 K450A/R451A in mammalian cellsDepositorInsertORF68
TagsN-terminal 2xStrep-Tag IIExpressionMammalianMutationAmino acids K450 and R451 mutated to alaninePromoterCMVAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-2xStrep-ORF68 K174A/R179A/K182A
Plasmid#162639PurposeExpresses N-terminal 2x-Strep-tagged ORF68 K174A/R179A/K182A in mammalian cellsDepositorInsertORF68
TagsN-terminal 2xStrep-Tag IIExpressionMammalianMutationAmino acids K174, R179, and K182 mutated to alani…PromoterCMVAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-2xStrep-ORF68 R14A/K310A
Plasmid#162642PurposeExpresses N-terminal 2x-Strep-tagged ORF68 R14A/K310A in mammalian cellsDepositorInsertORF68
TagsN-terminal 2xStrep-Tag IIExpressionMammalianMutationAmino acids R14 and K310 mutated to alaninePromoterCMVAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-2xStrep-ORF68 K395A/K396A
Plasmid#162643PurposeExpresses N-terminal 2x-Strep-tagged ORF68 K395A/K396A in mammalian cellsDepositorInsertORF68
TagsN-terminal 2xStrep-Tag IIExpressionMammalianMutationAmino acids K395 and K396 mutated to alaninePromoterCMVAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUE1-TSP-ORF68 K435A
Plasmid#162651PurposeExpresses ORF68 K435A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage siteDepositorInsertORF68
TagsN-terminal 2xStrep-Tag II and HRV 3C siteExpressionMammalianMutationAmino acid K435 mutated to alaninePromoterCMVAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUE1-TSP-ORF68 K450A/R451A
Plasmid#162653PurposeExpresses ORF68 K450A/R451A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage siteDepositorInsertORF68
TagsN-terminal 2xStrep-Tag II and HRV 3C siteExpressionMammalianMutationAmino acids K450 and R451 mutated to alaninePromoterCMVAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-2xStrep-ORF68 C191A/C192A
Plasmid#162632PurposeExpresses N-terminal 2x-Strep-tagged ORF68 C191A/C192A in mammalian cellsDepositorInsertORF68
TagsN-terminal 2xStrep-Tag IIExpressionMammalianMutationAmino acids C191 and C192 mutated to alaninePromoterCMVAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-BXG-altTAG
Plasmid#158132PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertBFP-GFP fragment
ExpressionYeastMutationCloned in a TAG codon at the first serine residue…PromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-BXG-altTAG2
Plasmid#158134PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertBFP-GFP fragment
ExpressionYeastMutationCloned in a TAG codon at the second serine residu…PromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-BXG-altTAG3
Plasmid#158136PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertBFP-GFP fragment
ExpressionYeastMutationCloned in a TAG codon at the third alanine residu…PromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-BXG-altTAG4
Plasmid#158137PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertBFP-GFP fragment
ExpressionYeastMutationCloned in a TAG codon at the third serine residue…PromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
AlkB-D135G
Plasmid#160434PurposeA plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to GlyDepositorInsertE. coli AlkB
TagsHis-tagExpressionBacterialMutationChanged Aspartic acid 135 to Glycine; deleted the…PromoterT7Available SinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtS1HR1AtR2NtB2
Plasmid#160890PurposeExpression of N. tabacum Rubisco small subunit (rbcS-SH1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-S1H
Tags6xHisExpressionBacterialPromoterrbcS-SH1: T7, chaperones: T13Available SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtT4aR1AtR2NtB2
Plasmid#160887PurposeExpression of N. tabacum Rubisco small subunit (rbcS-T4a), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-T4a
ExpressionBacterialPromoterrbcS-T4a: T7, chaperones: T10Available SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-NLS-mTurquoise2dC11-408LOV2-Ja(delta)-NES21-WPRE
Plasmid#159961PurposeoptoNES construct with modified mTurquoise2-LOV2 RETDepositorInsertNLS-mTurquoise2dC11-408LOV2-Ja(delta)-NES21
UseAAVExpressionMammalianMutationsyntheticPromoterhSyn1Available SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-NLS-mTurquoise2dC11-408LOV2-Ja(delta)-NES21-WPRE
Plasmid#159965PurposeoptoNES construct with modified mTurquoise2-LOV2 RETDepositorInsertNLS-mTurquoise2dC11-408LOV2-Ja(delta)-NES21
UseAAVExpressionMammalianMutationsyntheticPromoterCMV-IEAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-NLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21-WPRE
Plasmid#159964PurposeoptoNES construct with modified mTurquoise2-LOV2 RETDepositorInsertNLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21
UseAAVExpressionMammalianMutationsyntheticPromoterCMV-IEAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-mTurquoise2-optoJNKi-V416I-WPRE
Plasmid#159970Purposeoptogenetic JNK inhibitor with extended relaxation timeDepositorInsertmTurquoise2-optoJNKi-V416I
UseAAVExpressionMammalianMutationsyntheticPromoterhSyn1Available SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-NLS-mTurquoise2dC8-408LOV2-Ja(delta)-NES21-WPRE
Plasmid#159958PurposeoptoNES construct with modified mTurquoise2-LOV2 RETDepositorInsertNLS-mTurquoise2dC8-408LOV2-Ja(delta)-NES21
UseAAVExpressionMammalianMutationsyntheticPromoterhSyn1Available SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-NLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21-WPRE
Plasmid#159957PurposeoptoNES construct with modified mTurquoise2-LOV2 RETDepositorInsertNLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21
UseAAVExpressionMammalianMutationsyntheticPromoterhSyn1Available SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-NLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21-WPRE
Plasmid#159960PurposeoptoNES construct with modified mTurquoise2-LOV2 RETDepositorInsertNLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21
UseAAVExpressionMammalianMutationsyntheticPromoterhSyn1Available SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-NLS-mTurquoise2dC9-408LOV2-Ja(delta)-NES21-WPRE
Plasmid#159959PurposeoptoNES construct with modified mTurquoise2-LOV2 RETDepositorInsertNLS-mTurquoise2dC9-408LOV2-Ja(delta)-NES21
UseAAVExpressionMammalianMutationsyntheticPromoterhSyn1Available SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-NLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21-WPRE
Plasmid#159963PurposeoptoNES construct with modified mTurquoise2-LOV2 RETDepositorInsertNLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21
UseAAVExpressionMammalianMutationsyntheticPromoterCMV-IEAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-mScarlet-optoJNKi-WPRE
Plasmid#159968Purposeoptogenetic JNK inhibitor with increased RET from LOV2 to reduce actuation sensitivity, detectable without activationDepositorInsertmScarlet-optoJNKi
UseAAVExpressionMammalianMutationsyntheticPromoterhSyn1Available SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-NLS-YPET-optoNES.F434L-WPRE
Plasmid#159975Purposeoptical regulation of nuclear export with shorter relaxation timeDepositorInsertNLS-YPET-optoNES.F434L
UseAAVExpressionMammalianMutationF434LPromoterhSyn1Available SinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-NLS-YPET-optoNES.V416I-WPRE
Plasmid#159973Purposeoptical regulation of nuclear export with extended relaxation timeDepositorInsertNLS-YPET-optoNES.V416I
UseAAVExpressionMammalianMutationV416IPromoterhSyn1Available SinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-NLS-YPET-optoNES.I427V-WPRE
Plasmid#159974Purposeoptical regulation of nuclear export with shorter relaxation timeDepositorInsertNLS-YPET-optoNES.I427V
UseAAVExpressionMammalianMutationI427VPromoterhSyn1Available SinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-Ypet-optoJNKi-WPRE
Plasmid#159967Purposeoptogenetic JNK inhibitor with increased RET from LOV2 to reduce actuation sensitivity, detectable without activationDepositorInsertYpet-optoJNKi
UseAAVExpressionMammalianMutationsyntheticPromoterhSyn1Available SinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDUAL-Pnmt1-Ost4-GFP
Plasmid#154368PurposeYeast expression of Ost4DepositorInsertOst4 (ost4 Fission Yeast)
ExpressionYeastAvailable SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDUAL_2_lacZ
Plasmid#155173Purposesee pDUAL_lacZ: exchanged origin of replication (pBR322) and introduced bom siteDepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialAvailable SinceOct. 9, 2020AvailabilityAcademic Institutions and Nonprofits only