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pGEX CblC-SH2

Plasmid

#46414

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Cblc CBLC (CBLC Human)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 8-326

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-HA-ERK5 K84R

Plasmid

#53171

Purpose

Expresses GST-HA tagged human ERK5 K84R from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK5 (MAPK7 Human)

Tags

GST and HA

Expression

Bacterial

Mutation

K84R

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT-TO-FH-Nsp8

Plasmid

#157695

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp8 (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pNTKV- Utf1-KI-3'UTR-2A-tdTomato-RBGpA

Plasmid

#128834

Purpose

Knock-in of tdTomato into mouse Utf1 locus

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

tdTomato into Utf1 locus (Utf1 Mouse)

Use

Mouse Targeting

Available Since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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TRBV12-4

Plasmid

#123446

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV12-4 (TRBV12-4 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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TRAV2

Plasmid

#123369

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRAV2 (TRAV2 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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SIN40C.TRE.dTomato.miR-125b-2.PGK.sfGFP.P2A.Tet3G

Plasmid

#169315

Purpose

Doxycycline-inducible expression of miR-125b-2 with dTomato as reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

miR-125b-2 (MIR125B2 Human)

Use

Lentiviral

Tags

dTomato

Promoter

TRE

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCK669

Plasmid

#192638

Purpose

Expresses dxCas9-NG and MCP-SoxS(R93A/S101A) on p15A-CmR

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

Inserts

Sp.pCas9-dxCas9-NG
BBa_J23107-MCP-SoxS(R93A, S101A)

Use

CRISPR and Synthetic Biology

Mutation

SoxS has R93A and S101A mutations and dxCas9-NG h…

Promoter

BBa_J23107 and Sp.pCas9

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX Grb7-SH2

Plasmid

#46444

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Grb7 (GRB7 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 417-532

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Blk-SH2

Plasmid

#46407

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Blk (BLK Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 119-224

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX p85b(N)-SH2

Plasmid

#46467

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

p85b(N) (PIK3R2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 318-431

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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TRCN0000231737

Plasmid

#78162

Purpose

Luciferase control

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

Luciferase

Use

RNAi

Promoter

hU6 and hU6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
GFP SDPR

Plasmid

#68398

Purpose

Expression of fluorescently tagged SDPR in mammalian cells

Depositor

Rob Parton

Article

Bastiani et al J Cell Biol. 2009 Jun 29;185(7):1259-73.

Insert

SDPR (Sdpr Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 24, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT-TO-FH-Nsp15

Plasmid

#157721

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp15 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp15 (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 10, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pPN098

Plasmid

#91625

Purpose

Express sgRNA targeting human HCN1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

HCN1 (HCN1 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN099

Plasmid

#91626

Purpose

Express sgRNA targeting human HCN1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

HCN1 (HCN1 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HRPT2 136X

Plasmid

#11049

Depositor

Matthew Meyerson

Article

Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

Insert

HRPT2 136X (CDC73 Human)

Tags

flag

Expression

Mammalian

Mutation

truncation mutant with a stop codon introduced at…

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only
pGEX p85b(C)-SH2

Plasmid

#46466

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

p85b(C) (PIK3R2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 610-728

Promoter

Tac

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Socs4-SH2

Plasmid

#46507

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Socs4 (SOCS6 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 376-498

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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HBM-mVEGF-C-HisTag-pENTR

Plasmid

#64066

Purpose

Plasmid encodes mature form of murine VEGF-C preceded by honey bee melittin secretion signal; entry vector to Gateway cloning system.

Depositor

Joanna Bereta

Article

Klaus et al Protein Expr Purif. 2015 Mar 7. pii: S10

Insert

vascular endothelial growth factor C (Vegfc Mouse)

Tags

His-tag

Expression

Insect

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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