165,271 results

1. pAS_4xMma PylT_FLAG-Mma PylRS

   Plasmid

   #140009

   Purpose

   Plasmid with 4xMmaPylT cassette and Mma PylRS for amber suppression; for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   Mma PylRS (pylS Methanosarcina mazei)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EF1

   Available Since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOpen-MMLV_RT (lack H)

   Plasmid

   #165556

   Purpose

   Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity. Comparable to SuperScriptII from Thermo Fisher.

   Depositor

   Jennifer Molloy, FreeGenes Project, Drew Endy

   Insert

   Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain)
   

   Use

   Synthetic Biology

   Available Since

   Aug. 16, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pcDNA3-CFP

   Plasmid

   #13030

   Purpose

   Mammalian expression vector for cloning your gene fused to CFP

   Depositor

   Doug Golenbock

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Nov. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-TrxRFP2

   Plasmid

   #172322

   Purpose

   an improved fluorescent biosensor for thioredoxin redox

   Depositor

   Huiwang Ai

   Article

   Pang et al Biochemistry. 2022 Mar 1;61(5):377-384.

   Insert

   TrxRFP2, an improved version from TrxRFP1
   

   Tags

   His-tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX-CMV-Galpha-oA ONE-GO

   Plasmid

   #189732

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Inserts

   Galpha-oA ONE-GO
   GNAO (Gnao1 Rat)
   

   Use

   Lentiviral

   Mutation

   I250 (silent mutation in YFP)

   Available Since

   Jan. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pcDNA3/Myc-DNMT1

   Plasmid

   #36939

   Purpose

   Mammalian expression of human DNMT1 with myc tag

   Depositor

   Arthur Riggs

   Article

   Li et al J Biol Chem. 2006 Jul 14;281(28):19489-5

   Insert

   DNMT1 (DNMT1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CaMKIIa-hM3D(Gq)-mCherry

   Plasmid

   #50476

   Purpose

   Gq-coupled hM3D DREADD fused with mCherry under the control of CaMKIIa promoter

   Depositor

   Bryan Roth

   Has Service

   AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   hM3D(Gq)-mCherry (CHRM3 Human)
   

   Use

   AAV

   Tags

   mCherry

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   CaMKIIa

   Available Since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMRX-IB-HaloTag7-mGFP

   Plasmid

   #184903

   Purpose

   Stable expression of HaloTag7-mGFP in mammalian cells to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

   Insert

   monomeric EGFP
   

   Tags

   HaloTag7

   Expression

   Mammalian

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRBC-sfGFP wild type

   Plasmid

   #174075

   Purpose

   Plasmid with p15a origin of replication expressing codon optimized superfolder GFP with C-terminal His6 tag, under T7 promoter

   Depositor

   Ryan Mehl

   Article

   Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

   Insert

   Superfolder GFP
   

   Tags

   His-6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. MGAT2-RpHLuorin2

    Plasmid

    #171718

    Purpose

    Cis-/medial-Golgi expression of ratiometric pHLuorin2

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Insert

    Ratiometric pHLuorin2 fused to MGAT2 (MGAT2 Human)
    

    Tags

    ratiometric pHluorin2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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