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pBIG1a_3xFlag-6His-nsp15 (SARS-CoV-2)

Plasmid

#169166

Purpose

Baculoviral transfer vector for the expression of SARS-CoV-2 nsp15 in insect cells

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2465-2479

Insert

3xFlag-6His-nsp15 (ORF1ab Synthetic, SARS-CoV-2)

Tags

3xFlag-6His

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-Drosha (NLS-P426)

Plasmid

#171538

Purpose

Encodes mutant human Drosha protein

Depositor

Yan Zeng

Article

Zhang et al Biochim Biophys Acta Gene Regul Mech. 20

Insert

DROSHA (DROSHA Human)

Expression

Mammalian

Mutation

an SV40 NLS sequence (PKKKRKVGI) insertion into t…

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 hSlp4-a C2AB

Plasmid

#40037

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a C2AB (SYTL4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

C2A and C2B domains (353-671)

Promoter

CMV promoter

Available Since

Oct. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC26A10_STOP

Plasmid

#161150

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC26A10 (SLC26A10 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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BMPR2_HUMAN_D0

Plasmid

#79709

Purpose

This plasmid encodes the kinase domain of BMPR2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

BMPR2 (BMPR2 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pDONR221-SLC35E4_STOP

Plasmid

#161311

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35E4 (SLC35E4 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC35E1_STOP

Plasmid

#161264

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35E1 (SLC35E1 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX Jak3-SH2

Plasmid

#46449

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Jak3 (JAK3 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 366-494

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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KC1E_HUMAN_D0

Plasmid

#79724

Purpose

This plasmid encodes the kinase domain of KC1E. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KC1E (CSNK1E Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
DAPK3_HUMAN_D0

Plasmid

#79721

Purpose

This plasmid encodes the kinase domain of DAPK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

DAPK3 (DAPK3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
EPHB3_HUMAN_D0

Plasmid

#79732

Purpose

This plasmid encodes the kinase domain of EPHB3. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

EPHB3 (EPHB3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
EPHB2_HUMAN_D0

Plasmid

#79697

Purpose

This plasmid encodes the kinase domain of EPHB2. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

EPHB2 (EPHB2 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
CD8a-cNLS-EGFP

Plasmid

#86052

Purpose

A mammalian expression plasmid expressing CD8a luminal and transmembrane domains followed by cNLS (classical nuclear localization signal) and C-terminus EGFP.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

CD8a (CD8A Human)

Tags

GFP

Expression

Mammalian

Mutation

CD8a luminal and transmembrane along with two SV4…

Promoter

CMV

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pZS1[GalS-T,RbsR-T]

Plasmid

#60773

Purpose

Contains PIq driving expression GalS-T, and PIq driving expression of RbsR-T.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

GalS-T
GalS-T

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. TAN DBD with GalS LB…

Available Since

July 10, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX Socs2-SH2

Plasmid

#46505

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Socs2 (SOCS2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 28-162

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pETM33_Nsp3c_SUD-N

Plasmid

#156469

Purpose

Bacterial expression of Sars-CoV2 Nsp3c_SUD-N protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp3c_SUD-N (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pETM33_Nsp3c_SUD-M

Plasmid

#156470

Purpose

Bacterial expression of Sars-CoV2 Nsp3c_SUD-M protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp3c_SUD-M (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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AT1G56120 X070_pECIA14

Plasmid

#114860

Purpose

Prey vector AT1G56120 X070_pECIA14 should be used with bait vector AT1G56120 X070_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G56120 (AT1G56120 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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AT1G56120 X070_pECIA2

Plasmid

#115060

Purpose

Bait vector AT1G56120 X070_pECIA2 should be used with prey vector AT1G56120 X070_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G56120 (AT1G56120 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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PXY X058_pECIA2

Plasmid

#115115

Purpose

Bait vector PXY X058_pECIA2 should be used with prey vector PXY X058_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G61480

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT2G24130 L4_pECIA2

Plasmid

#115121

Purpose

Bait vector AT2G24130 L4_pECIA2 should be used with prey vector AT2G24130 L4_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT2G24130 (AT2G24130 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
AT3G47090 L5_pECIA2

Plasmid

#115122

Purpose

Bait vector AT3G47090 L5_pECIA2 should be used with prey vector AT3G47090 L5_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT3G47090 (AT3G47090 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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XPS1 L6_pECIA2

Plasmid

#115123

Purpose

Bait vector XPS1 L6_pECIA2 should be used with prey vector XPS1 L6_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT3G47110 (AT3G47110 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
AT3G47570 L7_pECIA2

Plasmid

#115124

Purpose

Bait vector AT3G47570 L7_pECIA2 should be used with prey vector AT3G47570 L7_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT3G47570 (AT3G47570 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
AT3G47580 L8_pECIA2

Plasmid

#115125

Purpose

Bait vector AT3G47580 L8_pECIA2 should be used with prey vector AT3G47580 L8_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT3G47580 (AT3G47580 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
ERL1 C11_pECIA2

Plasmid

#115129

Purpose

Bait vector ERL1 C11_pECIA2 should be used with prey vector ERL1 C11_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G62230

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
AT2G16250 K9_pECIA2

Plasmid

#115132

Purpose

Bait vector AT2G16250 K9_pECIA2 should be used with prey vector AT2G16250 K9_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT2G16250 (AT2G16250 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
AT4G39270 K12_pECIA2

Plasmid

#115133

Purpose

Bait vector AT4G39270 K12_pECIA2 should be used with prey vector AT4G39270 K12_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT4G39270 (AT4G39270 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
RLK7/KUK2 C12_pECIA2

Plasmid

#115095

Purpose

Bait vector RLK7/KUK2 C12_pECIA2 should be used with prey vector RLK7/KUK2 C12_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G09970

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
RGI5 X047_pECIA2

Plasmid

#115097

Purpose

Bait vector RGI5 X047_pECIA2 should be used with prey vector RGI5 X047_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G34110 (AT1G34110 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge