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IVT-MCP-Phi29

Plasmid

#207456

Purpose

in vitro transcription of mRNA

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

Insert

MCP-Phi29

Expression

Bacterial

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pLG015 (RADAR)

Plasmid

#157893

Purpose

Expresses the RADAR defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

RADAR subtype containing auxillary gene rdrD

Expression

Bacterial

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ150

Plasmid

#69301

Purpose

Gateway entry vector with pcoCas9 (Plant codon optimized)

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

pcoCas9 (plant codon-optimized)

Use

CRISPR

Tags

3XFLAG

Mutation

NLS fusion

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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PB-GFP1-10-HygR

Plasmid

#223675

Purpose

To check the split GFP signals in mitochondria (1)

Depositor

Juan Melero-Martin

Article

Lin et al Nature. 2024 May;629(8012):660-668. doi:

Insert

GFP1-10

Expression

Mammalian

Promoter

cmv

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV3flag8SHARPIN

Plasmid

#50014

Purpose

Mammalian expression of human SHARPIN with a c-terminal 3xFLAG tag, and hygromycin selection.

Depositor

Martin Dorf

Article

Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2

Insert

SHANK-associated RH domain interactor (SHARPIN Human)

Tags

3 x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 31, 2013

Availability

Academic Institutions and Nonprofits only

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ALKBH5-dCas9

Plasmid

#134783

Purpose

Expression fusion protein ALKBH5-dCas9 in Mammalian cells

Depositor

Shu-Bing Qian

Article

Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

Insert

ALKBH5 and inactive dCas9

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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PCMV-flag-Caspase11

Plasmid

#21145

Depositor

Junying Yuan

Article

Li et al Nat Cell Biol. 2007 Mar . 9(3):276-86.

Insert

Caspase11 (Casp4 Mouse)

Tags

flag

Expression

Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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FTO-dCas9

Plasmid

#134781

Purpose

Expression fusion protein FTO-dCas9 in Mammalian cells

Depositor

Shu-Bing Qian

Article

Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

Insert

FTO and inactive dCas9

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-Talin1

Plasmid

#26724

Depositor

Anna Huttenlocher

Article

Franco et al Nat Cell Biol. 2004 Oct . 6(10):977-83.

Insert

Talin (Tln1 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 30, 2010

Availability

Academic Institutions and Nonprofits only

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TRX1 His C32S C35S

Plasmid

#21286

Depositor

Wang Min

Article

Liu et al Circ Res. 2002 Jun 28. 90(12):1259-66.

Insert

TRX1 (TXN Human)

Tags

His

Expression

Mammalian

Mutation

C32S C35S

Available Since

July 30, 2009

Availability

Academic Institutions and Nonprofits only
pGL3-Dbp

Plasmid

#186822

Purpose

Fluorescent reporter for Dbp expression

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

dbp promoter (Dbp Mouse)

Use

Luciferase

Promoter

dbp promoter

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ143

Plasmid

#69295

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 3 gRNAs

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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p5E-mcs2

Plasmid

#138362

Purpose

Gateway compatible 5' entry clone contains mutli-cloning sites sequence2.

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

sequence multi cloning sites

Expression

Mammalian

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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FKBP-eGFP-TAZ

Plasmid

#221213

Purpose

part A of TAZ-SPARK-OFF

Depositor

Xiaokun Shu

Article

Chung et al Proc Natl Acad Sci U S A. 2024 Feb 13;12

Insert

FKBP-eGFP-TAZ

Expression

Mammalian

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMGE-T7

Plasmid

#153021

Purpose

Single-plasmid multi-gene expression system. Each genes under the control of a T7 and the lacO promoter sequences.

Depositor

Vito Valiante

Article

Kufs et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Promoter

T7

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Myc-IQGAP1

Plasmid

#30118

Depositor

David Sacks

Article

Ho et al J Biol Chem. 1999 Jan 1. 274(1):464-70.

Insert

IQGAP1 (IQGAP1 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only

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px459V2.0_Conc3

Plasmid

#134458

Purpose

Empty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + Puro

Depositor

Daniel Stange

Article

Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP Tubulin K40R

Plasmid

#105303

Purpose

To study effects of acetylation-null tubulin

Depositor

Kenneth Yamada

Article

Joo et al Nat Commun. 2014 Mar 25;5:3510. doi: 10.

Insert

alpha tubulin (TUBA1B Human)

Tags

EGFP

Expression

Mammalian

Mutation

K40R

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pGGA016

Plasmid

#228231

Purpose

Level 0 for 5xOp (GreenGate A module)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

5xOp

Use

Synthetic Biology

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-Sec23A

Plasmid

#66609

Purpose

GFP fusion of Sec23A

Depositor

David Stephens

Article

Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

Insert

Sec23A (SEC23A Human)

Tags

EGFP

Expression

Mammalian

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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