We narrowed to 10,386 results for: plasmids 101

1. Let7R-IRE-TREAT

   Plasmid

   #196934

   Purpose

   Reverse complement of let7-miRNA binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT.

   Depositor

   Jeffrey Chao

   Article

   Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

   Insert

   Renilla luciferase
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. CAG-EGFP-Otx2shRNA2

   Plasmid

   #73982

   Purpose

   Otx2 shRNA2 (based on mir155) was inserted into the 3'UTR of EGFP.

   Depositor

   Connie Cepko

   Article

   Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

   Insert

   EGFP-Otx2-shRNA2
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKM149

   Plasmid

   #134178

   Purpose

   Bacterial expression of his-CENP-C 510-end

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Cell. 2014 Jul 17;158(2):397-411. doi: 1

   Insert

   his-CENP-C 510-end (CENPC Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   aa 510-end

   Promoter

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5/FRT/TO/intron/FlagCter

   Plasmid

   #113548

   Purpose

   Mammalian expression plasmid that can be used with the Flp-In T-REx system.

   Depositor

   Blerta Xhemalce

   Article

   Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

   Insert

   Flag
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSR35

   Plasmid

   #69256

   Purpose

   phsp16.41::CRE for integration on ttTi14024, Chr X

   Depositor

   Sander van den Heuvel

   Article

   Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

   Insert

   Cre recombinase
   

   Use

   Cre/Lox; Mossci

   Tags

   Expression

   Worm

   Mutation

   codon-optimzed index 1.0

   Promoter

   hsp16.41

   Available Since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC_h7SK Mma PylT (UUA)

   Plasmid

   #154783

   Purpose

   plasmid with single copy of Mma PylT (UUA anticodon)

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

   Insert

   Mma PylT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   U25C and UUA anticodon

   Promoter

   h7SK

   Available Since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSR28

   Plasmid

   #69253

   Purpose

   phlh-8::CRE for integration on ttTi14024, Chr X

   Depositor

   Sander van den Heuvel

   Article

   Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

   Insert

   Cre recombinase
   

   Use

   Cre/Lox; Mossci

   Tags

   Expression

   Worm

   Mutation

   codon-optimzed index 1.0

   Promoter

   hlh-8

   Available Since

   Oct. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-oChD-citrine

   Plasmid

   #50976

   Purpose

   AAV transfer vector with mammalian codon-optimized ChD-citrine between AAV2 ITR

   Depositor

   Roger Tsien

   Article

   Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

   Insert

   oChD-citrine
   

   Use

   AAV

   Tags

   citrine

   Expression

   Mutation

   ChD gene is mammalian codon optimized

   Promoter

   human synapsin promoter

   Available Since

   Feb. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. Bxb1-GA-Donor_CBh

   Plasmid

   #241376

   Purpose

   Bxb1-GA donor plasmid for cloning of payloads <20 kb with CBh promoter for expressing landing pad enrichment marker

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Bxb1-GA-CAG Donor

    Plasmid

    #241374

    Purpose

    Bxb1-GA donor plasmid with CAG promoter, WPRE and polyA signal for constitutive transgene expression (empty)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Bxb1-GT-CAG Donor

    Plasmid

    #241373

    Purpose

    Bxb1-GT donor plasmid with CAG promoter, WPRE and polyA signal for constitutive transgene expression (empty)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Bxb1-GA-Donor_UbC

    Plasmid

    #241375

    Purpose

    Bxb1-GA donor plasmid for cloning of payloads <20 kb with UbC promoter for expressing landing pad enrichment marker

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. YEE_GFP

    Plasmid

    #228184

    Purpose

    Yeast episomal expression vector with type '234' GFP drop out site. For use in assembling protein expression cassettes via golden gate assembly.

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. YEE_RFP

    Plasmid

    #228185

    Purpose

    Yeast episomal expression vector with type '234' RFP drop out site. For use in assembling protein expression cassettes via golden gate assembly.

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAV-EF1α-loxP-GCaMP6f-loxP-WPRE

    Plasmid

    #233288

    Purpose

    AAV vector with EF1α promoter and Cre-Out GCaMP6f, enabling to express GCaMP6f in Cre-negative cells.

    Depositor

    Dayu Lin

    Article

    Fang et al Neuron. 2018 Apr 4;98(1):192-207.e10. do

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1α

    Available Since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. TRE-pfv-Sapphire

    Plasmid

    #231957

    Purpose

    The plasmid containing TRE-pfv-Sapphire for the doxycycline-inducible expression of genetically encoded multimeric nanoparticles (GEM)

    Depositor

    Michael Shtutman

    Article

    Korunova et al bioRxiv [Preprint]. 2024 Nov 18:2024.11.

    Insert

    genetically encoded multimeric nanoparticles
    

    Use

    Lentiviral; Doxocycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE promoter

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. Bxb1-GT_TRE-mScarlet

    Plasmid

    #229796

    Purpose

    Bxb1-GT donor plasmid for doxycycline-inducible mScarlet expression

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mScarlet
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. Bxb1-GA_TRE-jRCaMP1b

    Plasmid

    #229798

    Purpose

    Bxb1-GA donor plasmid for doxycycline-inducible expression of jRCaMP1b calcium reporter.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    jRCaMP1b
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available Since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-22b(+) pCopA sfgfp

    Plasmid

    #226374

    Purpose

    Plasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with native RBS (ribosome binding site).

    Depositor

    Urartu Seker

    Article

    Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

    Insert

    sfGFP
    

    Use

    Tags

    6x HisTag

    Expression

    Bacterial

    Mutation

    Promoter

    pCopA

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSMART-HCKan-yibDp-VHH72

    Plasmid

    #202467

    Purpose

    Expresses the nanobody VHH72 after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    nanobody VHH72
    

    Use

    Tags

    6X-His

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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