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We narrowed to 18,759 results for: nls

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Showing: 9521 - 9560 of 18759 results

  2. Unfused click editor construct; PCV2-EcKlenow fusion - pCMV-T7-PCV2-EcKlenow (JO1421)

    Plasmid
    #217804
    Purpose
    Unfused click editor construct expressing PCV2-EcKlenow, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCV2-linker-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217804-plasmid-map-sequence-id-432897

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  3. pDM028

    Plasmid
    #216809
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and hph selectable marker.
    Depositor
    Ken Sawin
    Publication
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Expression
    Bacterial
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216809-plasmid-map-sequence-id-430135

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  4. pDM068

    Plasmid
    #216811
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and NAT selectable marker.
    Depositor
    Ken Sawin
    Publication
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Expression
    Bacterial
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216811-plasmid-map-sequence-id-430142

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  12. pMSCV-BLU-VIPR

    Plasmid
    #220497
    Purpose
    Retroviral plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222-2A-Thy1.1 and the C120 element followed by cloning sites for ribozyme flanked gRNA (RGR).
    Depositor
    Alexander Espinosa
    Publication
    Pulgarin et al Nucleic Acids Res. 2025 Mar 20;53(6):gka
    Type
    Empty backbone
    Use
    CRISPR and Retroviral
    Expression
    Mammalian
    Available since
    Aug. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    220497-plasmid-map-sequence-id-442817

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  13. PX459v3-eSpCas9(1.1)

    Plasmid
    #178800
    Purpose
    High-fidelity eSpCas9(1.1) with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Publication
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178800-plasmid-map-sequence-id-354839

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  15. gRNA3_NIPBL_Cas9-hGem-for_N-terminal_tagging

    Plasmid
    #217662
    Purpose
    expresses Cas9-hGem and guideRNA for N terminal tagging of NIPBL
    Depositor
    Daniel Gerlich
    Publication
    Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA3 for N terminal tagging of NIPBL (NIPBL Human)
    Use
    CRISPR
    Expression
    Bacterial and Mammalian
    Promoter
    U6
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    217662-plasmid-map-sequence-id-440854

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  16. pafG-CBE-3T

    Plasmid
    #220374
    Purpose
    for hA3A-nCas9 and gRNA expression in Aspergillus flavus
    Depositor
    Xue Gao
    Publication
    Zhao et al J Am Chem Soc. 2023 Jan 11;145(1):413-42
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    hA3A-nCas9
    tRNA and gRNA scaffold
    Use
    CRISPR
    Promoter
    Ptef1 and tRNA
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    220374-plasmid-map-sequence-id-440033

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  21. XLone-Puro CCND2-T2A-luciferase-P2A-eGFP

    Plasmid
    #179843
    Purpose
    Tunable and temporal expression control of CCND2, luciferase and nuclear eGFP
    Depositor
    Xiaoping Bao
    Publication
    Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CCND2 (CCND2 Human)
    Use
    Luciferase and Synthetic Biology
    Tags
    eGFP and luciferase
    Expression
    Mammalian
    Promoter
    TRE3G
    Available since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    179843-plasmid-map-sequence-id-354943

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  23. pUbi_dCas9-BFP-KRAB-gRNA

    Plasmid
    #221503
    Purpose
    CRISPRi machinery fish codon optimized expressing plasmid
    Depositor
    Alessandro Ori
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-tagBFP-KRAB
    Use
    Fish expression
    Mutation
    D10A and H839A point mutations
    Promoter
    Zebrafish Ubiquitin promoter
    Available since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    221503-plasmid-map-sequence-id-439415

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  28. AAVS1_Cas9-hGem_gRNA2

    Plasmid
    #217659
    Purpose
    expresses Cas9-hGem and guideRNA targeting the human AAVS1 safe harbour locus
    Depositor
    Daniel Gerlich
    Publication
    Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA2 targeting the human AAVS1 safe harbour locus
    Use
    CRISPR
    Expression
    Bacterial and Mammalian
    Promoter
    U6
    Available since
    July 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    217659-plasmid-map-sequence-id-438226

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  29. pCAG-eSpCas9-2A-GFP-sgRNA-DNA-PKcs

    Plasmid
    #220493
    Purpose
    To generate DNA-PKcs KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against DNA-PKcs exon 1.
    Depositor
    Sébastien Britton
    Publication
    Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting DNA-PKcs (PRKDC) exon 1 (PRKDC Human)
    Use
    CRISPR
    Promoter
    U6
    Available since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    220493-plasmid-map-sequence-id-437553

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  30. TDP-REGv2 Cryptic PE-Max

    Plasmid
    #216163
    Purpose
    Expression of prime editor PEMax (Plasmid #174820) only in cells with TDP-43 loss of function. Note: It is not recommended to produce lentivirus containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Publication
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PEMax for prime editing with internal cryptic exon
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216163-plasmid-map-sequence-id-437751

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  33. gTBEv4

    Plasmid
    #220620
    Purpose
    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv4) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Publication
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…
    Promoter
    hU6, EF1a
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220620-plasmid-map-sequence-id-436970

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  34. pLenti-nSpCas9-evoCDA1

    Plasmid
    #209042
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where evoCDA1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Publication
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    evoCDA1-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    209042-plasmid-map-sequence-id-413615

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  36. pLenti-nSpCas9-TadA8e

    Plasmid
    #209044
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized adenine base editor where TadA8e (ABE8e) is fused to the N-terminus of SpCas9(D10A)-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Publication
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    TadA8e-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    209044-plasmid-map-sequence-id-413636

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  39. apt-ABE8e

    Plasmid
    #219937
    Purpose
    Mammalian expression of apt-ABE8e protein (Com_93aa-linker_TadA8e) with P2A-EGFP marker
    Depositor
    Alexis Komor
    Publication
    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Com_93-aa_TadA8e
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    219937-plasmid-map-sequence-id-435507

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  40. nCas9-SpRY_P2A-mCherry

    Plasmid
    #219935
    Purpose
    Mammalian expression of SpRY-nCas9 with P2A-mCherry marker for use with MOBE systems
    Depositor
    Alexis Komor
    Publication
    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Sp-nCas9(D10A)-SpRY_P2A-mCherry
    Use
    CRISPR
    Tags
    SV40 bpNLS and SV40 bpNLS-P2A-mCherry
    Expression
    Mammalian
    Mutation
    nCas9(D10A)
    Promoter
    CMV
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    219935-plasmid-map-sequence-id-435502

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Showing: 9521 - 9560 of 18759 results