Addgene

We narrowed to 705 results for: PRS3;

Showing: 461 - 480 of 705 results

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pJW1669

Plasmid

#112043

Purpose

CEN/ARS plasmid for GAL-inducible NSG2-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

NSG2

Use

Tags

V5-TEV-EGFP

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Availability

Academic Institutions and Nonprofits only

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pJW1672

Plasmid

#112046

Purpose

CEN/ARS plasmid for GAL-inducible PST1-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

PST1

Use

Tags

V5-TEV-EGFP

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Availability

Academic Institutions and Nonprofits only

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pJW1674

Plasmid

#112048

Purpose

CEN/ARS plasmid for GAL-inducible SWP1-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

SWP1

Use

Tags

V5-TEV-EGFP

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Availability

Academic Institutions and Nonprofits only

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pPM47 (UPR-RFP CEN/ARS URA3)

Plasmid

#20132

Depositor

Feroz Papa

Article

Merksamer et al Cell. 2008 Nov 28. 135(5):933-47.

Insert

UPR-RFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 11, 2009

Availability

Academic Institutions and Nonprofits only

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pJW1662

Plasmid

#112050

Purpose

C-terminal tag with - AID*(AtIAA17_71-114)-HA and OsTIR1 expression cassette marked with LEU

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Inserts

IAA17
TIR1

Use

Tags

HA

Expression

Yeast

Mutation

only amino acids 71-114

Promoter

ADH1 and None

Available since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pTPGI_dCas9

Plasmid

#64279

Purpose

encodes yeast-optimized dCas9 synthetic transcription factor

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

Yeast-optimized dCas9

Use

Tags

SV40 NLS

Expression

Yeast

Mutation

D10A & H840A relative to S. pyogenes Cas9

Promoter

pTPGI

Available since

May 5, 2015

Availability

Academic Institutions and Nonprofits only

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pMET17p-CRU313

Plasmid

#131163

Purpose

For ScMET17 promoter regulated expression of Cub-R-URA3 fused bait proteins, centromeric ARS plasmid, HIS3 complements the histidine auxotrophy of S. cerevisiae his mutant

Depositor

Nils Johnsson

Article

Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

Type

Empty backbone

Use

Tags

CRU

Expression

Yeast

Mutation

Promoter

Available since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pPS1622

Plasmid

#8854

Depositor

Pamela Silver

Article

Prinz et al J Cell Biol. 2000 Aug 7. 150(3):461-74.

Insert

Sec63-sGFP (SEC63 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

sGFP=S65T V163A mutant of GFP

Promoter

Available since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
pMG13

Plasmid

#122932

Purpose

Expression of the regulator tTA.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

tTA

Use

Tags

Expression

Yeast

Mutation

Promoter

Pcln3

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pJK59-ATG18-GFP

Plasmid

#42529

Depositor

James Hurley

Article

Baskaran et al Mol Cell. 2012 Aug 10;47(3):339-48. doi:

Insert

Atg18 (ATG18 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

none

Promoter

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pJLI-sup35

Plasmid

#1210

Depositor

Susan Lindquist

Article

Liu et al Nature 1999 Aug 5;400(6744):573-6.

Insert

sup35 5' and 3' flanking regions (SUP35 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

sup35 gene is not included in this plasmid. Only…

Promoter

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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pSL6 - Brr2 del247 WT

Plasmid

#90256

Purpose

protein expression

Depositor

Christine Guthrie

Article

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

BRR2

Use

Tags

TAP

Expression

Yeast

Mutation

deleted first 247 amino acids

Promoter

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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CDC12(K351N)

Plasmid

#1163

Depositor

Susan Lindquist

Article

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

CDC12 (CDC12 Budding Yeast)

Use

Tags

266bp of upstream sequence

Expression

Yeast

Mutation

K351N

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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GAL-CDC12(K351N)

Plasmid

#1166

Depositor

Susan Lindquist

Article

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

CDC12 (CDC12 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

K351N

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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CDC12(E368Q)

Plasmid

#1167

Depositor

Susan Lindquist

Article

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

CDC12 (CDC12 Budding Yeast)

Use

Tags

266bp of upstream sequence

Expression

Yeast

Mutation

E368Q

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pGALSc104(G217S/T499I)

Plasmid

#1151

Depositor

Susan Lindquist

Article

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

HSP104 (HSP104 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

G217S/T499I

Promoter

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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pSL10 - Brr2 del422 WT

Plasmid

#90260

Purpose

protein expression

Depositor

Christine Guthrie

Article

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

BRR2

Use

Tags

TAP

Expression

Yeast

Mutation

deleted first 422 amino acids

Promoter

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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GAL-CDC12(E368Q)

Plasmid

#1164

Depositor

Susan Lindquist

Article

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

CDC12 (CDC12 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

E368Q

Promoter

GAL

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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RMC

Plasmid

#1173

Depositor

Susan Lindquist

Article

Sondheimer et al Mol Cell 2000 Jan;5(1):163-72.

Insert

Rnq1-Sup35 fusion (SUP35, RNQ1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

*(see below). Contains Rnq aa's 153-393 and…

Promoter

Available since

Jan. 21, 2005

Availability

Academic Institutions and Nonprofits only

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pPS934

Plasmid

#8855

Depositor

Pamela Silver

Article

Kahana et al Mol Biol Cell. 1998 Jul . 9(7):1741-56.

Insert

NUF2-sGFP (NUF2 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only