1,878 results

1. pLT 652

   Plasmid

   #59997

   Purpose

   Expresses a hybrid dsRNA corresponding to the Caenorhabditis elegans dhc-3/him-8 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progeny

   Depositor

   Lisa Timmons

   Article

   Timmons et al FEBS Lett. 2014 Jul 24. pii: S0014-5793(

   Inserts

   dhc-3 (dhc-3 Nematode)
   him-8 (him-8 Nematode)
   

   Expression

   Bacterial

   Mutation

   partial genomic

   Available Since

   April 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJH4531

   Plasmid

   #132366

   Purpose

   Prgef-1 DAF-2 unc-54 3' UTR C.elegans pan-neural expression of DAF-2. This plasmid has replaced plasmid pJH664 from the Zhen Lab.

   Depositor

   Mei Zhen

   Article

   Hung et al Development. 2014 Apr;141(8):1767-79. do

   Insert

   DAF-2 (daf-2 Nematode)
   

   Tags

   none

   Expression

   Worm

   Promoter

   Prgef-1

   Available Since

   Sept. 22, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pCG144

   Plasmid

   #21376

   Depositor

   Geraldine Seydoux

   Article

   Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

   Insert

   mCherry in pDONR201
   

   Use

   Gateway

   Available Since

   June 19, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDR3.10

   Plasmid

   #19367

   Depositor

   Geraldine Seydoux

   Article

   Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

   Insert

   msp-142 ORF+3' in pDONR201
   

   Expression

   Worm

   Available Since

   Dec. 16, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDC20

   Plasmid

   #131366

   Purpose

   expression of MEG-3 (aa1-862) in C. elegans

   Depositor

   Geraldine Seydoux

   Article

   Smith et al Elife. 2016 Dec 3;5. pii: 21337. doi: 10

   Insert

   meg-3 (aa1-862) (meg-3 Nematode)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   April 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCFJ1208

   Plasmid

   #44490

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

   Insert

   Minimal Mos1 [Peft-3 tdTomato(NLS) tbb-2 UTR, unc-119]
   

   Use

   Gateway expression clone

   Available Since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJA254

   Plasmid

   #21507

   Depositor

   Julie Ahringer

   Article

   Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

   Insert

   pmex-5::GFP-F64LS65T::(Gly)5Ala
   

   Use

   Multisite gateway

   Available Since

   Aug. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJH2217

   Plasmid

   #179251

   Purpose

   Psra-11::TWK-18(gf)-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of TWK-18-wCherry

   Depositor

   Mei Zhen

   Article

   Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

   Insert

   TWK-18 (twk-18 Nematode)
   

   Tags

   wCherry

   Expression

   Worm

   Mutation

   M280I

   Promoter

   Psra-11

   Available Since

   Feb. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCFJ2442 - [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)

   Plasmid

   #159858

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSEM416 - mosTI unc-119 P_UPN - gfp(nls) - tbb-2

    Plasmid

    #182359

    Purpose

    Positive control for mosTI(unc-119). unc-119 rescue and pan-neuronal nuclear GFP expression after mosTI insertion.

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    Ultra-pan-neuronal positive control pUNP::gfp(NLS)
    

    Expression

    Worm

    Promoter

    Promoter Ultra Pan Neuronal

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDO001

    Plasmid

    #198828

    Purpose

    Inhibition of synaptic vessel release due to disruption of synaptic vessel function by reactive oxygen species production.

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::miniSOG-103L-VAMP2-mCherry::let-858 3'UTR
    

    Expression

    Worm

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPD133.47

    Plasmid

    #27510

    Depositor

    Andrew Fire

    Article

    Zhang et al Dev Biol. 2010 Aug 15. 344(2):603-10.

    Insert

    pmyo-3::cfp::lacZ::unc-54_3'UTR
    

    Expression

    Worm

    Available Since

    March 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMLS260

    Plasmid

    #73685

    Purpose

    Punc-17::2xNLS-FLP-D5 Expression vector

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    Punc-17::2xNLS-FLP-D5::let-858 3'-UTR
    

    Use

    Flp/frt

    Expression

    Worm

    Mutation

    G5D

    Promoter

    Punc-17

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. YW249_Pzipt17-loxP-H2BGFP-miniMOS

    Plasmid

    #196063

    Purpose

    a plasmid modified from miniMOS vector pCFJ910. the modification including insertions of SL2::H2B::GFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and GFP cassettes.

    Depositor

    Wenxing Yang

    Article

    Li et al PeerJ. 2023 May 17;11:e15433. doi: 10.77

    Inserts

    H2B::GFP
    NeoR
    Minimal Mos1
    zipt17p::zipt17::zipt17 3UTR
    

    Expression

    Worm

    Available Since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJJS010

    Plasmid

    #188333

    Purpose

    PCR template for repair templates to tag a gene with a mKate2 and mIAA7 AID degron using homology-directed repair for C. elegans.

    Depositor

    Mike Boxem

    Article

    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

    Insert

    Linker::mKate2::mIAA7
    

    Expression

    Bacterial

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJJS004

    Plasmid

    #188327

    Purpose

    PCR template for repair templates to tag a gene with a mCherry and mIAA7 AID degron using homology-directed repair for C. elegans.

    Depositor

    Mike Boxem

    Article

    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

    Insert

    Linker::mCherry::mIAA7
    

    Expression

    Bacterial

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJW1846

    Plasmid

    #154326

    Purpose

    Multi-cassette for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::GFP^SEC^AID*::3xFLAG
    

    Use

    CRISPR and Cre/Lox

    Expression

    Worm

    Mutation

    F+E mutations in sgRNA

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJH4733

    Plasmid

    #178898

    Purpose

    Pmyo-3 DAF-2 unc-54 3' UTR C.elegans muscle expression of DAF-2

    Depositor

    Mei Zhen

    Article

    Hung et al Development. 2014 Apr;141(8):1767-79. do

    Insert

    DAF-2 (daf-2 Nematode)
    

    Expression

    Worm

    Promoter

    Pmyo-3

    Available Since

    Jan. 5, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pCFJ606 - pDESTR4-R3(ttTi14024, X)

    Plasmid

    #34862

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

    Insert

    ttTi14024 targeting
    

    Use

    Worm targeting

    Available Since

    June 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSH1

    Plasmid

    #90295

    Purpose

    Expresses the fat-7 gene in C. elegans intestine

    Depositor

    Anne Brunet

    Article

    Han et al Nature. 2017 Apr 13;544(7649):185-190. d

    Insert

    fat-7 (fat-7 Nematode)
    

    Tags

    No

    Expression

    Worm

    Promoter

    ges-1

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge