2,162 results

1. SI1-Unc5b

   Plasmid

   #28214

   Depositor

   Gavin Wright

   Article

   Crosnier et al BMC Biol. 2010 Jun 4;8:76. doi: 10.1186/

   Insert

   Unc5b mouse monoclonal antibody (unc5b Zebrafish)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPRISM-Cre-cmlc2-mRFP

   Plasmid

   #117793

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   Cre
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMT-ubb-NLS-BirA-2a-mCherry

   Plasmid

   #79886

   Purpose

   Ubiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Tags

   3x HA

   Expression

   Bacterial

   Mutation

   Promoter

   Ubiquitin

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCS-foxc1a-2a-nlstdTomato

   Plasmid

   #132970

   Purpose

   full-length of foxc1a in frame with viral 2a peptide and nuclear localized tdTomato in pCS expression vector

   Depositor

   Nathan Lawson

   Article

   Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

   Inserts

   tdTomato
   foxc1a (foxc1a Zebrafish)
   

   Use

   Tags

   NLS and myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMT-MCS-NLS-BirA-2A-mCherry_Ras

   Plasmid

   #80059

   Purpose

   MCS for cloning promoter to drive HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Zebrafish biotagging

   Tags

   3x HA

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPRISM-Stop-cmlc2-TagBFP

   Plasmid

   #117774

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   3x3 Stop sequence
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Zebrafish-gRNA-0005

   Plasmid

   #42245

   Purpose

   gRNA targeted to zebrafish gene gsk3b

   Depositor

   Keith Joung

   Article

   Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Insert

   gRNA-gsk3b (gsk3b Zebrafish)
   

   Use

   CRISPR; Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available Since

   Jan. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

8. CACNA1F_L (OZ507)

   Plasmid

   #26957

   Depositor

   Keith Joung

   Article

   Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

   Insert

   Zinc finger array targeting cacna1f (cacna1f Zebrafish)
   

   Use

   Zebrafish targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EGFP-Bin1a

   Plasmid

   #176013

   Purpose

   Mammalian expression of zebrafish EGFP-Bin1a. Parton lab clone HYA

   Depositor

   Rob Parton

   Article

   Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

   Insert

   Bin1a
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SI2-Unc5b

    Plasmid

    #28215

    Depositor

    Gavin Wright

    Article

    Crosnier et al BMC Biol. 2010 Jun 4;8:76. doi: 10.1186/

    Insert

    Unc5b mouse monoclonal antibody (unc5b Zebrafish)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPRISM-Fuse-gcry-TagBFP

    Plasmid

    #117783

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    mRFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. M16 pCS-twhh-bGFP

    Plasmid

    #17153

    Depositor

    Randall Moon

    Article

    Du et al J Cell Biol. 1997 Oct 6. 139(1):145-56.

    Insert

    twhh promoter (shhb Zebrafish)
    

    Use

    Tags

    bGFP

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

13. p3xFLAG-zDrosha

    Plasmid

    #171540

    Purpose

    Encodes Zebrafish Drosha protein

    Depositor

    Yan Zeng

    Article

    Li et al Biochem Biophys Res Commun. 2019 Jun 18;

    Insert

    drosha (rnasen Zebrafish)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p3E-rab32a

    Plasmid

    #109689

    Purpose

    Multisite gateway vector for 3' tagging with rab32a

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    rab32a (rab32a Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ZE7 Pax2

    Plasmid

    #17056

    Depositor

    Randall Moon

    Article

    Kelly et al Dev Genet. 1995. 17(2):129-40.

    Insert

    pax b (pax2a Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pDestTol2-actinb-kcnj13-EGFP

    Plasmid

    #164940

    Purpose

    Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj13 fused with EGFP at the C terminus

    Depositor

    GuangJun Zhang

    Article

    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

    Insert

    kcnj13 (kcnj13 Zebrafish)
    

    Use

    Tol2 transposon destination vector

    Tags

    EGFP

    Expression

    Mutation

    Stop codon deleted

    Promoter

    actinb (actb2) promoter

    Available Since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pME-foxc1a

    Plasmid

    #132971

    Purpose

    full-length of foxc1a without stop codon in pmE Gateway vector

    Depositor

    Nathan Lawson

    Article

    Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

    Insert

    foxc1a (foxc1a Zebrafish)
    

    Use

    Gateway vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSP64T acvr1ba*

    Plasmid

    #185409

    Purpose

    Constitutively active form of acvr1ba (Nodal receptor). For in vitro transcription (SP6)

    Depositor

    Nicolas David

    Article

    Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

    Insert

    acvr1ba (acvr1ba Zebrafish)
    

    Use

    In vitro synthesis of mrna

    Tags

    Expression

    Mammalian

    Mutation

    changed Threonine 206 to Aspartic acid

    Promoter

    Available Since

    June 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pT2-cryR;mafbbCE1-basEgfp

    Plasmid

    #90137

    Purpose

    contains mafbb enhancer sequence and a basal promoter driving expression of eGFP

    Depositor

    Nathan Lawson

    Article

    Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

    Insert

    mafbbE1basEgfp
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Zebrafish-gRNA-0008

    Plasmid

    #42248

    Purpose

    gRNA targeted to zebrafish gene tia1l

    Depositor

    Keith Joung

    Article

    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    gRNA-tia1l (tia1l Zebrafish)
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only