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pTrc99A-Rat FABP1

Plasmid

#13577

Depositor

Martin Scanlon

Article

Velkov et al Protein Expr Purif. 2005 Nov . 44(1):23-

Insert

Rat liver fatty acid binding protein (Fabp1 Rat)

Expression

Bacterial

Available Since

March 15, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-Fos DD

Plasmid

#8968

Depositor

John Blenis

Article

Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

Insert

c-Fos (Fos Rat)

Tags

FLAG

Expression

Mammalian

Mutation

S362D, S374D

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-ERK2 L198A L232A

Plasmid

#8978

Depositor

John Blenis

Article

Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

Insert

ERK2 (Mapk1 Rat)

Tags

HA

Expression

Mammalian

Mutation

L198A, L232A

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
pORANGE Bsn-GFP KI

Plasmid

#139665

Purpose

Endogenous tagging of Bassoon: C-terminal (amino acid position: F3938)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pORANGE Rims1-GFP KI

Plasmid

#131494

Purpose

Endogenous tagging of RIM1: C-terminal (amino acid position: S1615)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Rims1 Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pORANGE mEos3.2-Camk2a KI

Plasmid

#131493

Purpose

Endogenous tagging of CaMKIIa: N-terminal (amino acid position: before startcodon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and mEos3.2 donor (Camk2a, Camk2a Mouse, Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-CACNA1A KI

Plasmid

#131480

Purpose

Endogenous tagging of CaV2.1, P/Q: N-terminal (amino acid position: G10)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Cacna1a, Cacna1a Mouse, Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FLEX(frt)-GCaMP6f-WPRE

Plasmid

#118273

Purpose

pAAV vector for flippase dependent GCaMP6f expression under the control of EF1a promoter

Depositor

Naoshige Uchida

Article

Menegas et al Nat Neurosci. 2018 Sep 3. pii: 10.1038/s

Insert

GCaMP6f

Use

AAV

Tags

6xHis (N terminal on insert), T7 epitope, and Xpr…

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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Rlucrarr3-Flash1

Plasmid

#74129

Purpose

FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 40 of Arrestin3.

Depositor

Louis Luttrell

Article

Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

Insert

Arrestin3

Tags

Renilla luciferase (rLuc)

Expression

Mammalian

Mutation

Insertion of FlAsH motif (CCPGCC) following amino…

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only
pMK285 (Stag-3FLAG-Bsr)

Plasmid

#72801

Purpose

C-terminal tagging with Stag-3FLAG using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

Stag-3FLAG-Bsr

Use

CRISPR

Tags

Stag-3FLAG

Expression

Mammalian

Available Since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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CD200-Cd4d3+4-COMP-blac-3xFLAG-6his

Plasmid

#71471

Purpose

A positive control receptor prey (rat CD200) EXPRESs plasmid for The Basic AVEXIS kit

Depositor

Gavin Wright

Article

Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

Insert

CD200 (Cd200 Rat)

Tags

6xHis tag, Cd4d3+4, beta-lactamase, and triple FL…

Expression

Mammalian

Promoter

CMV

Available Since

March 8, 2016

Availability

Academic Institutions and Nonprofits only

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msfGFP-Cx43

Plasmid

#69024

Purpose

Expresses rat Cx43 (Gja1 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to msfGFP (FP fused to NT of connexin). CMV promoter.

Depositor

David Spray

Article

Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

Insert

Cx43 (Gja1 Rat)

Tags

sfGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pRc/CMV HA TrkA

Plasmid

#39977

Depositor

Yves-Alain Barde

Article

Bibel et al EMBO J. 1999 Feb 1;18(3):616-22.

Insert

TrkA (Ntrk1 Rat)

Tags

BM40 signal sequence and HA

Expression

Mammalian

Mutation

endogenous signal sequence was replaced with BM40…

Promoter

CMV

Available Since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNAI-GAL4-CREB

Plasmid

#46769

Purpose

Expression of GAL4 fusion to wild type CREB

Depositor

Richard Maurer

Article

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

GAL4(1-147)-CREB (Creb1, GAL4 Rat, Budding Yeast)

Tags

GAL4

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pFLAG ARMS

Plasmid

#24086

Depositor

Moses Chao

Article

Kong et al J Neurosci. 2001 Jan 1. 21(1):176-85.

Insert

ARMS (Kidins220 Rat)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pCIneoMyc rat SAPAP1

Plasmid

#40215

Depositor

Yutaka Hata, Yoshimi Takai

Article

Takeuchi et al J Biol Chem. 1997 May 2;272(18):11943-51

Insert

SAPAP1 (Dlgap1 Rat)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
Cav2.2e[a10, 18a, Δ24a, 31a, 37b, 46]

Plasmid

#26568

Depositor

Diane Lipscombe

Article

Pan et al J Neurosci. 2000 Jul 1. 20(13):4769-75.

Insert

calcium channel alpha-1B subunit (Cacna1b Rat)

Tags

6XHis and V5

Expression

Mammalian

Mutation

contains exons a10, 18a, 31a, 37b, and 46. Del…

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
PKC zeta dNPS

Plasmid

#21250

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC zeta (Prkcz Rat)

Tags

HA

Expression

Mammalian

Mutation

delta 1-203

Available Since

Nov. 10, 2009

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-CaMKIIbeta(A303R)

Plasmid

#21224

Depositor

Tobias Meyer

Article

Shen et al Science. 1999 Apr 2. 284(5411):162-6.

Insert

CaMKIIbeta(A303R) (Camk2b Rat)

Tags

GFP

Expression

Mammalian

Mutation

CaMKIIbeta(A303R) (Lab plasmid CF 0002)

Available Since

July 29, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA5-TO-PV-NLS-Dsred

Plasmid

#16341

Depositor

Anton Bennett

Article

Rodrigues et al J Biol Chem. 2007 Jun 8. 282(23):17061-8

Insert

PV-DsRed-NLS (Pvalb Rat)

Tags

Myc and NLS

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only