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Addgene

We narrowed to 3,314 results for: GCT

Showing: 921 - 960 of 3314 results
  1. SGEP-sh-Hs-PHGDH-1883

    Plasmid
    #188669
    Purpose
    shRNA
    Depositor
    Insert
    shPHGDH (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. pLKO.1-blast shGFP

    Plasmid
    #110419
    Purpose
    shGFP control, silence GFP gene, blasticidin selection.
    Insert
    GFP
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 (RNA PolIII)
    Available since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
  3. pCfb13199

    Plasmid
    #219874
    Purpose
    The base plasmid of TUNEYALI forTF09
    Depositor
    Insert
    Contains gRNA targeting TF09 (YALI1_F29652g) and homologous arm matching TF09
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
  4. pCfb13239

    Plasmid
    #219912
    Purpose
    The base plasmid of TUNEYALI for TF49
    Depositor
    Insert
    Contains gRNA targeting TF49 (YALI1_C04143g) and homologous arm matching TF49
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. pCfb13247

    Plasmid
    #219918
    Purpose
    The base plasmid of TUNEYALI for TF57
    Depositor
    Insert
    Contains gRNA targeting TF57 (YALI1_D17249g) and homologous arm matching TF57
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. pCfb13233

    Plasmid
    #219906
    Purpose
    The base plasmid of TUNEYALI for TF43
    Depositor
    Insert
    Contains gRNA targeting TF43 (YALI1_C14774g) and homologous arm matching TF41
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. pCfb13231

    Plasmid
    #219905
    Purpose
    The base plasmid of TUNEYALI for TF41
    Depositor
    Insert
    Contains gRNA targeting TF41 (YALI1_D00747g) and homologous arm matching TF40
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
  8. pCfb13222

    Plasmid
    #219897
    Purpose
    The base plasmid of TUNEYALI for TF32
    Depositor
    Insert
    Contains gRNA targeting TF32 (YALI1_F33301g) and homologous arm matching TF32
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
  9. pCfb13227

    Plasmid
    #219902
    Purpose
    The base plasmid of TUNEYALI for TF37
    Depositor
    Insert
    Contains gRNA targeting TF37 (YALI1_D01890g) and homologous arm matching TF37
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
  10. mU6-sgSik1_2nd-hU6-sgNT

    Plasmid
    #177213
    Purpose
    Expresses a Sik1-targeting (mU6) and a non-targeting (hU6) gRNAs and Cre recombinase
    Depositor
    Insert
    sgSik1_2nd/sgNT1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    mU6/hU6
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
  11. mU6-sgSik1_1st-hU6-sgNT

    Plasmid
    #177212
    Purpose
    Expresses a Sik1-targeting (mU6) and non-targeting (hU6) gRNAs, and Cre-recombinase
    Depositor
    Insert
    sgSik1_1st/sgNT1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    mU6/hU6
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
  12. pZNF449-R.1.0-gDNA

    Plasmid
    #132469
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Insert
    ZNF449 (ZNF449 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
  13. pZNF3.1.0-gDNA

    Plasmid
    #132440
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Insert
    ZNF3 (ZNF3 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
  14. PX458_ZNF3_1

    Plasmid
    #72363
    Purpose
    Encodes gRNA for 3' target of human ZNF3 along with Cas9 with 2A GFP
    Insert
    ZNF3 (ZNF3 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
  15. tet-pLKO.puro_shGFP

    Plasmid
    #110421
    Purpose
    shGFP control, silence GFP gene, doxycycline inducible, puromycin selection
    Insert
    GFP
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    H1/TO (RNA PolIII)
    Available since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
  16. tet-pLKO.puro_shLuc

    Plasmid
    #110422
    Purpose
    shLuc control, silence Luc gene, doxycycline inducible, puromycin selection
    Insert
    Luciferase
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    H1/TO (RNA PolIII)
    Available since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
  17. pX330 Mouse 3' Hp1a gRNA

    Plasmid
    #127898
    Purpose
    WT Cas9 Vector targeting the 3' end of the mouse Hp1a gene
    Depositor
    Insert
    gRNA/Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
  18. pX601-miniCMV-SaCas9-U6-LacZvsSaCas9 sgRNA

    Plasmid
    #107049
    Purpose
    All-in-one vectors expressing both SaCas9 and LacZ sgRNA
    Depositor
    Insert
    SaCas9
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
  19. BII-gR-PnTW

    Plasmid
    #133356
    Purpose
    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications) coexpressed with tdTomato-NLS
    Depositor
    Insert
    tdTomato-NLS
    Use
    Tags
    HA-tagged tdTomato
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available since
    Jan. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  20. pX601-miniCMV-SaCas9-U6-YFPvsSaCas9 sgRNA

    Plasmid
    #107050
    Purpose
    All-in-one vectors expressing both SaCas9 and YFP sgRNA
    Depositor
    Insert
    SaCas9
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
  21. pSB700-ACTC1-Puro

    Plasmid
    #128324
    Purpose
    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (human cells)
    Depositor
    Insert
    gRNA against human ACTC1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
  22. pHnS KI;Gria1 Donor;3xV5 KO;Template

    Plasmid
    #240293
    Purpose
    KI:Gria1 Donor:3xV5 KO:Template
    Depositor
    Insert
    KI gRNA for Gria1
    Use
    AAV
    Tags
    Expression
    Mutation
    NA
    Promoter
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
  23. pHnS 2comp Donor;smFP-V5 KO;Gphn#1

    Plasmid
    #240307
    Purpose
    Donor:smFP-V5 KO:Gphn#1
    Depositor
    Insert
    KO gRNAs for Gphn
    Use
    AAV
    Tags
    Expression
    Mutation
    NA
    Promoter
    Available since
    July 11, 2025
    Availability
    Academic Institutions and Nonprofits only
  24. mPLD3-sgRNA-Cas9-mcherry

    Plasmid
    #199700
    Purpose
    encodes sgRNA for mouse PLD3 KO, (target 217-223aa) plus Cas9-P2A-mCherry
    Depositor
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    P2A-RFP
    Expression
    Mammalian
    Mutation
    Promoter
    U6 promoter
    Available since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
  25. pCAS-mak10 gRNA

    Plasmid
    #160385
    Purpose
    Expresses guide RNA for targeting MAK10 in yeast
    Depositor
    Insert
    mak10 gRNA
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    RNA pol III promoter (tRNA-Tyr)
    Available since
    Feb. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
  26. HCP7

    Plasmid
    #166109
    Purpose
    This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Whi5 and the other targets the C-terminus of Hta2.
    Depositor
    Insert
    Whi5-sg106 (WHI5 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
  27. pSpCas9(BB)-2A-Blast_HP1beta_gRNA

    Plasmid
    #127908
    Purpose
    WT Cas9 Vector with Blasticidin Selection Marker targeting the 5' end of the human HP1beta gene
    Depositor
    Insert
    gRNA for Human 5' HP1beta
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
  28. pICSL4723_ WRKY28_1

    Plasmid
    #126887
    Purpose
    Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.
    Depositor
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
  29. pENTRY4_ WRKY28_1

    Plasmid
    #126899
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 15, 2019
    Availability
    Academic Institutions and Nonprofits only
  30. bU6-sgSik1_1st-mU6-sgSik2-hU6-sgSik3_1st

    Plasmid
    #177232
    Purpose
    Expresses Sik1(bU6), Sik2 (mU6) and Sik3 (hU6) gRNAs and Cre-recombinase
    Depositor
    Insert
    sgSik1_1st/sgSik2/sgSik3_1st
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
  31. bU6-sgMark2-mU6-sgMark3-hU6-sgMark4

    Plasmid
    #177235
    Purpose
    Expresses Mark2 (bU6), Mark3 (mU6) and Mark4 (hU6) gRNAs and Cre-recombinase
    Depositor
    Insert
    sgMark2/sgMark3/sgMark4
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    Jan. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
  32. ipUSEPR-sg-Hs-PCNA

    Plasmid
    #188686
    Purpose
    control sgRNA
    Depositor
    Insert
    sgPCNA (PCNA Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  33. lentiGuideSLC35C1-neo

    Plasmid
    #220868
    Purpose
    encodes CRISPR sgRNA targeting human SLC3C1
    Depositor
    Insert
    SLC35C1 (SLC35C1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
  34. LT3GEPIR-MSH2shRNA1

    Plasmid
    #220587
    Purpose
    To inducibly knockdown MSH2 expression
    Depositor
    Insert
    MSH2 shRNA1 (MSH2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    TRE3G (TetOP) promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
  35. ipUSEPR-sg-Hs-TMPRSS2-A5

    Plasmid
    #188689
    Purpose
    sgRNA
    Depositor
    Insert
    sgTMPRSS2 (TMPRSS2 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  36. pNSU299_CUP1-1

    Plasmid
    #166086
    Purpose
    Plasmid for constituive spCas9 and tet-inducible CUP1-1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Insert
    CUP1-1 (CUP1-1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
  37. pNSU299_LHS1

    Plasmid
    #166092
    Purpose
    Plasmid for constituive spCas9 and tet-inducible LHS1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Insert
    LHS1 (LHS1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  38. pNSU299_APL3

    Plasmid
    #166073
    Purpose
    Plasmid for constituive spCas9 and tet-inducible APL1-targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Insert
    APL3 (APL3 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
  39. pX335 Mouse 5' Srcap gRNA B

    Plasmid
    #127905
    Purpose
    Cas9 D10A Nickase Vector targeting the 5' end of the mouse Srcap gene
    Depositor
    Insert
    Srcap gRNA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 921 - 960 of 3314 results