We narrowed to 2,111 results for: D10

1. LLP791_PB_dCas9-SSSavi_BirA

   Plasmid

   #211786

   Purpose

   PiggyBac plasmid with dCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), and BirA, with Hygro selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   dCas9, Spy, Snoop, aGCN4, AviTags
   

   Use

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   dCas9 D10A and H840A

   Promoter

   pCAGG abd pEF1a

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSpCas9n(BB)-2A-Puro (PX462)

   Plasmid

   #48141

   Purpose

   NOTE: A new version of this plasmid is now available. See Addgene plasmid 62987

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Cas9 D10A nickase mutant

   Promoter

   Cbh

   Available since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pJMP1237

   Plasmid

   #119262

   Purpose

   for cloning new sgRNAs

   Depositor

   Jason Peters, Oren Rosenberg, Carol Gross

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   dcas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Promoter

   Available since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. EC2_4_dCas9_VPR_sgRNA

   Plasmid

   #163709

   Purpose

   Yeast low copy plasmid with dCas9, sgRNA expression cassette and VPR activation domains

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Inserts

   dCas9
   VPR
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Point mutations D10A and H840A

   Promoter

   ScTEF1

   Available since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. 3xFLAG-dCas9/pMSCVhyg

   Plasmid

   #134323

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR and Retroviral

   Tags

   3xFLAG tag and NLS

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLV hUbC-VP64 dCas9 VP64-T2A-GFP

   Plasmid

   #59791

   Purpose

   Co-expresses human optimized S. pyogenes dCas9 fused to two copies of VP64 and GFP

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Insert

   humanized VP64 dead Cas9 VP64 T2A GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D10A and H840A

   Promoter

   Available since

   Oct. 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1-CibN-dCas9-CibN

   Plasmid

   #60553

   Purpose

   Expresses CibN-dCas9-CibN in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

   Insert

   dCas9-CibN
   

   Use

   CRISPR

   Tags

   FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   inactivating Cas9 mutations D10A and H840A

   Promoter

   CMV

   Available since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA5FRT/Flag-OGT1

   Plasmid

   #29760

   Depositor

   Joan Conaway

   Article

   Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

   Insert

   OGT1 (OGT Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   N764D and K1010E

   Promoter

   Available since

   July 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDNAHygro(+)LAMP2B

   Plasmid

   #86029

   Purpose

   Expresses LAMP2B in Mammalian cells

   Depositor

   Janice Blum

   Article

   Zhou et al Immunity. 2005 May;22(5):571-81.

   Insert

   Lysosome-associated membrane protein 2b (LAMP2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNICKclos1.0

    Plasmid

    #73639

    Purpose

    Genome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824

    Depositor

    Sheng Yang

    Article

    Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

    Inserts

    Cas9 nickase
    sGRNA to pyrE
    

    Use

    E.coli-clostridium shuttle vector

    Tags

    Expression

    Mutation

    D10A

    Promoter

    j23119 and ptb

    Available since

    June 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. EC2_3_dCas9_Mxi1_sgRNA

    Plasmid

    #163708

    Purpose

    Yeast low copy plasmid with dCas9, sgRNA expression cassette and Mxi1 transcriptional repressor

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Inserts

    dCas9
    Mxi1+SV40 NLS
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. EC2_10_dCas9_VPR_HC_sgRNA

    Plasmid

    #163712

    Purpose

    Yeast high copy plasmid with dCas9, sgRNA expression cassette and VPR activation domains

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Inserts

    dCas9
    VPR
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET24a-ncOGT-FL

    Plasmid

    #190821

    Purpose

    For expression of full-length human ncOGT in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.

    Depositor

    Gerald Hart

    Article

    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Codon optimized for expression in E.coli

    Promoter

    T7

    Available since

    Jan. 9, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pTDH3-dCas9

    Plasmid

    #46920

    Purpose

    Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS controlled by TDH3 promoter

    Depositor

    Stanley Qi, Jonathan Weissman

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJMP1354

    Plasmid

    #119275

    Purpose

    for cloning new sgRNAs

    Depositor

    Jason Peters, Oren Rosenberg, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SadCas9 VPR

    Plasmid

    #188514

    Purpose

    Expresses FLAG tagged SadCas9 VPR

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A/N580A

    Promoter

    EF1a

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. BPK1179

    Plasmid

    #179296

    Purpose

    Expresses dCas9 fused to four DmrA domains

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

    Insert

    dCas9-DmrA(x4)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive Cas9)

    Promoter

    CAG

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pXPR_502_sgCD45

    Plasmid

    #158705

    Purpose

    CD45 CRISPRa control

    Depositor

    John Doench, David Root

    Article

    Orchard et al J Virol. 2018 Dec 10;93(1). pii: JVI.013

    Insert

    CD45 (PTPRC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pScI_dCas9-CDA_J23119-sgRNA

    Plasmid

    #108550

    Purpose

    Bacterial Target-AID vector with sgRNA

    Depositor

    Akihiko Kondo

    Article

    Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

    Inserts

    dCas9-PmCDA1
    Streptococcus pyogenes sgRNA
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    D10A and H840A for SpCas9

    Promoter

    J23119 and lambda OR

    Available since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJEC726

    Plasmid

    #190814

    Purpose

    CRISPRi backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.

    Depositor

    James Chappell

    Article

    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    dCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    S. pyogenes dCas9 codon optimized for Streptomyce…

    Promoter

    SP30

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. A3A-PBE

    Plasmid

    #119768

    Purpose

    Conversion of C to T in wheat plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    APOBEC3A-nCas9-NLS-UGI-NLS
    

    Use

    Tags

    NLS (flanking UGI)

    Expression

    Plant

    Mutation

    nCas9 has a mutation D10A and E945K in wild type …

    Promoter

    maize Ubiquitin-1

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_EYFP

    Plasmid

    #167898

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. SadCas9 KRAB

    Plasmid

    #188504

    Purpose

    Expresses FLAG tagged SadCas9 KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A/N580A

    Promoter

    EF1a

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. BC1209-pHR-dSV40-dCas9-14xGFP11-P2A-BFP

    Plasmid

    #164038

    Purpose

    Expression of dCas9-14xGFP11 in mammalian cells

    Depositor

    Baohui Chen

    Article

    Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

    Insert

    dCas9-14xGFP11
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and H840A in Cas9

    Promoter

    dSV40

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_mcherry

    Plasmid

    #167902

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. NAX_CAGGS_CAS9_m4_VPR_KanR neo

    Plasmid

    #167885

    Purpose

    PiggyBac compatible plasmid expressing dCas9-VPR

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pXSE901BG

    Plasmid

    #91714

    Purpose

    CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta and glyphosate-resistance

    Depositor

    Qi-Jun Chen

    Article

    Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. HiFi dCas9 VPR

    Plasmid

    #188510

    Purpose

    Expresses FLAG tagged HiFi dCas9 VPR

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A/R691A/H840A

    Promoter

    EF1a

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pdCASclos

    Plasmid

    #73640

    Purpose

    Transcriptional repression for gene Spo0A (CAC-2011) in Clostridium acetobutylicum ATCC 824

    Depositor

    Sheng Yang

    Article

    Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

    Inserts

    dCas9
    sgRNA to spo0A
    

    Use

    E.coli-clostridium shuttle vector

    Tags

    Expression

    Mutation

    D10A, H840A

    Promoter

    Pj23119 and ptb

    Available since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNAHygro(+)LAMP2AS

    Plasmid

    #86146

    Purpose

    Anti sense for LAMP2

    Depositor

    Janice Blum

    Article

    Zhou et al Immunity. 2005 May;22(5):571-81.

    Insert

    LAMP2a (LAMP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    contains first 525 bases of LAMP cDNA

    Promoter

    CMV

    Available since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Emi LC

    Plasmid

    #113666

    Purpose

    For transient expression of Emicizumab sequence (CAS# 1610943-06-0, KEGG# D10821), IgG4 bispecific antibody targeting FIX(a) & FX(a). Must be co-expressed with anti-FIX(a) and anti-FX(a) Emi HC's.

    Depositor

    Robert Peters

    Article

    Leksa et al J Thromb Haemost. 2019 Mar 18. doi: 10.1

    Insert

    Emicizumab light chain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. YE2-BE4max

    Plasmid

    #138156

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

    Insert

    rAPOBEC1(YE2)-nSpCas9-UGI-UGI
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Within rAPOBEC1 W90Y + R132E; within Cas9 D10A

    Promoter

    CMV

    Available since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. VP64-dCas9

    Plasmid

    #177171

    Purpose

    VP64 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    Umeh-Garcia et al Br J Cancer. 2022 Apr 19. pii: 10.1038/s

    Insert

    VP64-dCas9(D10A, H840A)
    

    Use

    CRISPR

    Tags

    NLS and NLS-3xFLag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. TagBFP2-FKBP-PIP5K1C_Kina-HD

    Plasmid

    #202741

    Purpose

    expresses recruitable fluorescent protein-tagged enzyme

    Depositor

    Gerry Hammond

    Article

    Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

    Insert

    mTagBFP2:FKBP1A(3-108):[GGSA]4GG:PIP5K1C(79-366)(D101R/R304D) (PIP5K1C Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. TagBFP2-FKBP-PIP5K1C_Kina-HDD316K

    Plasmid

    #202742

    Purpose

    expresses recruitable fluorescent protein-tagged enzyme

    Depositor

    Gerry Hammond

    Article

    Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

    Insert

    mTagBFP2:FKBP1A (3-108) : [GGSA]4GG : PIP5K1C(79-366) (D101R / R304D / D316K) (PIP5K1C Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pREDIT_Cas9n-MS2-BB_BbsI

    Plasmid

    #164804

    Purpose

    REDIT backbone for nickase Cas9n, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9n(D10A)-T2A-EGFP

    Depositor

    Le Cong

    Article

    Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. NAX_CAGGS_CAS9_m4_VPR_mcherry

    Plasmid

    #167886

    Purpose

    PiggyBac compatible plasmid expressing dCas9-VPR

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

38. pCMV-BE-VQRCas9

    Plasmid

    #101740

    Purpose

    expression of an optimized Cas9 for base-editing in zebrafish

    Depositor

    Shuo Lin

    Article

    Zhang et al Nat Commun. 2017 Jul 25;8(1):118. doi: 1

    Insert

    rAPOBEC1-XTEN-zCas9(VQR)-UGI-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, VQR mutations

    Promoter

    CMV

    Available since

    Jan. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pRK5-mFzd2

    Plasmid

    #42254

    Depositor

    Chris Garcia, Jeremy Nathans

    Article

    Yu et al Development. 2012 Dec 1;139(23):4383-94.

    Insert

    Frizzled 2 (Fzd2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. 3xFLAG-dCas9/pMSCVpuro

    Plasmid

    #134983

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR and Retroviral

    Tags

    3xFLAG tag and NLS

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. CREB3L3

    Plasmid

    #99509

    Purpose

    to express CREB3L3 in mammalian system

    Depositor

    Dong-Yan Jin

    Article

    Chin et al Nucleic Acids Res. 2005 Mar 30;33(6):185

    Insert

    CREB3L3 (Creb3l3 Mouse)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pJMP1333

    Plasmid

    #119268

    Purpose

    rfp "test" strain

    Depositor

    Jason Peters, Oren Rosenberg, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. dCBE4-gam

    Plasmid

    #124449

    Purpose

    Expresses dead Cas9 (dCas9)-CBE4-gam

    Depositor

    George Church

    Article

    Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

    Insert

    dCBE4-gam
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    Available since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pRS315e_pGal-dCas9-PmCDA1

    Plasmid

    #79615

    Purpose

    Expresses dCas9-PmCDA1 in yeast cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1

    Expression

    Yeast

    Mutation

    D10A and H840A for nuclease deficient Cas9

    Promoter

    pGal1

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. EE-BE4max

    Plasmid

    #138158

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

    Insert

    rAPOBEC1(EE)-nSpCas9-UGI-UGI
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Within rAPOBEC1 R126E + R132E; within Cas9 D10A

    Promoter

    CMV

    Available since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pJMP1187

    Plasmid

    #119256

    Purpose

    rfp "test" strain

    Depositor

    Jason Peters, Oren Rosenberg, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pBSE901

    Plasmid

    #91709

    Purpose

    CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta resistance

    Depositor

    Qi-Jun Chen

    Article

    Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pLicC-MBP-APETx2

    Plasmid

    #72668

    Purpose

    Periplasmic expression of APETx2 in E.coli

    Depositor

    Glenn King

    Article

    Anangi et al Mar Drugs. 2012 Jul;10(7):1605-18. doi:

    Insert

    APETx2
    

    Use

    Tags

    His tag, MBP, MalE signal sequence for periplasmi…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pGEL035

    Plasmid

    #137903

    Purpose

    STU2.0 SpyCas9(D10A)-PmCDA1 for plant genome base editing

    Depositor

    Yong Zhang

    Article

    Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pENTR1A hu_ncOGT WT

    Plasmid

    #154281

    Purpose

    Entry vector encoding non-tagged O-GlcNAc Transferase

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Gateway entry vector

    Tags

    2x FLAG and mKate2

    Expression

    Mutation

    WT OGT

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge